Experiment: D-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08515 and BWI76_RS08520 are separated by 5 nucleotides BWI76_RS08520 and BWI76_RS08525 are separated by 3 nucleotides BWI76_RS08525 and BWI76_RS08530 are separated by 105 nucleotides
BWI76_RS08515: BWI76_RS08515 - acyl-CoA thioesterase YbgC, at 1,803,387 to 1,803,791
_RS08515
BWI76_RS08520: BWI76_RS08520 - colicin transporter TolQ, at 1,803,797 to 1,804,480
_RS08520
BWI76_RS08525: BWI76_RS08525 - colicin transporter, at 1,804,484 to 1,804,912
_RS08525
BWI76_RS08530: BWI76_RS08530 - protein TolA, at 1,805,018 to 1,806,355
_RS08530
Position (kb)
1804
1805 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1803.764 kb on + strand at 1803.812 kb on - strand at 1803.823 kb on + strand at 1804.000 kb on - strand, within BWI76_RS08520 at 1804.358 kb on + strand, within BWI76_RS08520 at 1804.368 kb on + strand, within BWI76_RS08520 at 1804.421 kb on - strand at 1804.433 kb on + strand at 1804.486 kb on + strand at 1804.491 kb on + strand at 1804.546 kb on + strand, within BWI76_RS08525 at 1804.697 kb on + strand, within BWI76_RS08525 at 1804.753 kb on + strand, within BWI76_RS08525 at 1805.247 kb on + strand, within BWI76_RS08530
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine (N) remove 1,803,764 + +0.4 1,803,812 - -2.3 1,803,823 + -3.0 1,804,000 - BWI76_RS08520 0.30 -0.9 1,804,358 + BWI76_RS08520 0.82 -1.6 1,804,368 + BWI76_RS08520 0.83 +0.1 1,804,421 - -1.6 1,804,433 + -1.0 1,804,486 + -3.7 1,804,491 + -1.1 1,804,546 + BWI76_RS08525 0.14 -3.1 1,804,697 + BWI76_RS08525 0.50 -2.3 1,804,753 + BWI76_RS08525 0.63 -1.9 1,805,247 + BWI76_RS08530 0.17 -2.6
Or see this region's nucleotide sequence