Experiment: 2-Deoxy-D-ribonic lithium salt (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02220 and BPHYT_RS02225 are separated by 27 nucleotides BPHYT_RS02225 and BPHYT_RS02230 are separated by 47 nucleotides BPHYT_RS02230 and BPHYT_RS02235 overlap by 8 nucleotides BPHYT_RS02235 and BPHYT_RS02240 are separated by 94 nucleotides
BPHYT_RS02220: BPHYT_RS02220 - butyryl-CoA dehydrogenase, at 510,218 to 511,348
_RS02220
BPHYT_RS02225: BPHYT_RS02225 - short-chain dehydrogenase, at 511,376 to 512,053
_RS02225
BPHYT_RS02230: BPHYT_RS02230 - acetyl-CoA acetyltransferase, at 512,101 to 513,294
_RS02230
BPHYT_RS02235: BPHYT_RS02235 - beta carbonic anhydrase, at 513,287 to 514,063
_RS02235
BPHYT_RS02240: BPHYT_RS02240 - isocitrate dehydrogenase kinase/phosphatase, at 514,158 to 515,996
_RS02240
Position (kb) 512
513
514 Strain fitness (log2 ratio) -1
0
1
2 at 511.402 kb on - strand at 511.407 kb on + strand at 511.993 kb on - strand at 511.993 kb on - strand at 512.056 kb on - strand at 512.056 kb on - strand at 512.056 kb on - strand at 512.058 kb on + strand at 512.058 kb on + strand at 512.342 kb on + strand, within BPHYT_RS02230 at 512.825 kb on + strand, within BPHYT_RS02230 at 512.846 kb on + strand, within BPHYT_RS02230 at 512.912 kb on + strand, within BPHYT_RS02230 at 513.424 kb on - strand, within BPHYT_RS02235 at 513.496 kb on - strand, within BPHYT_RS02235 at 513.554 kb on - strand, within BPHYT_RS02235 at 513.564 kb on - strand, within BPHYT_RS02235 at 514.113 kb on + strand at 514.114 kb on - strand at 514.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxy-D-ribonic lithium salt (C) remove 511,402 - +0.0 511,407 + -0.4 511,993 - +0.2 511,993 - +1.4 512,056 - -1.5 512,056 - -0.1 512,056 - -0.2 512,058 + +2.3 512,058 + +0.9 512,342 + BPHYT_RS02230 0.20 +1.7 512,825 + BPHYT_RS02230 0.61 -0.4 512,846 + BPHYT_RS02230 0.62 +0.5 512,912 + BPHYT_RS02230 0.68 +0.3 513,424 - BPHYT_RS02235 0.18 -1.1 513,496 - BPHYT_RS02235 0.27 -0.6 513,554 - BPHYT_RS02235 0.34 +1.4 513,564 - BPHYT_RS02235 0.36 -0.4 514,113 + +0.4 514,114 - +0.2 514,114 - +0.1
Or see this region's nucleotide sequence