Experiment: Phosphomycin 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2551 and Shew_2552 are separated by 73 nucleotides Shew_2552 and Shew_2553 are separated by 118 nucleotides Shew_2553 and Shew_2554 are separated by 80 nucleotides
Shew_2551: Shew_2551 - homoserine O-succinyltransferase (RefSeq), at 3,008,237 to 3,009,202
_2551
Shew_2552: Shew_2552 - hypothetical protein (RefSeq), at 3,009,276 to 3,009,677
_2552
Shew_2553: Shew_2553 - short-chain dehydrogenase/reductase SDR (RefSeq), at 3,009,796 to 3,010,566
_2553
Shew_2554: Shew_2554 - outer membrane protein W (RefSeq), at 3,010,647 to 3,011,288
_2554
Position (kb)
3009
3010
3011 Strain fitness (log2 ratio)
-1
0
1
2 at 3008.806 kb on + strand, within Shew_2551 at 3008.847 kb on - strand, within Shew_2551 at 3009.230 kb on - strand at 3009.241 kb on - strand at 3009.392 kb on + strand, within Shew_2552 at 3009.427 kb on - strand, within Shew_2552 at 3009.486 kb on + strand, within Shew_2552 at 3009.549 kb on - strand, within Shew_2552 at 3009.549 kb on - strand, within Shew_2552 at 3009.958 kb on - strand, within Shew_2553 at 3010.645 kb on + strand at 3010.841 kb on + strand, within Shew_2554 at 3010.942 kb on - strand, within Shew_2554
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.12 mg/ml remove 3,008,806 + Shew_2551 0.59 +0.2 3,008,847 - Shew_2551 0.63 +0.4 3,009,230 - -1.5 3,009,241 - +2.3 3,009,392 + Shew_2552 0.29 +0.3 3,009,427 - Shew_2552 0.38 +0.8 3,009,486 + Shew_2552 0.52 +0.2 3,009,549 - Shew_2552 0.68 +0.8 3,009,549 - Shew_2552 0.68 +0.8 3,009,958 - Shew_2553 0.21 -1.2 3,010,645 + -0.1 3,010,841 + Shew_2554 0.30 -0.3 3,010,942 - Shew_2554 0.46 +1.0
Or see this region's nucleotide sequence