Experiment: Phosphomycin 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2369 and Shew_2370 are separated by 120 nucleotides Shew_2370 and Shew_2371 are separated by 111 nucleotides Shew_2371 and Shew_2372 are separated by 11 nucleotides Shew_2372 and Shew_2373 are separated by 299 nucleotides
Shew_2369: Shew_2369 - cyclic nucleotide-binding protein (RefSeq), at 2,769,980 to 2,771,827
_2369
Shew_2370: Shew_2370 - methyltransferase type 11 (RefSeq), at 2,771,948 to 2,772,607
_2370
Shew_2371: Shew_2371 - actP-like component of L-lactate and L-malate uptake system (from data) , at 2,772,719 to 2,774,437
_2371
Shew_2372: Shew_2372 - small component of L-lactate and L-malate uptake system (from data) , at 2,774,449 to 2,774,715
_2372
Shew_2373: Shew_2373 - DSBA oxidoreductase (RefSeq), at 2,775,015 to 2,775,647
_2373
Position (kb)
2772
2773
2774
2775 Strain fitness (log2 ratio)
-1
0
1 at 2771.751 kb on - strand at 2772.566 kb on + strand at 2772.703 kb on - strand at 2772.854 kb on + strand at 2773.707 kb on + strand, within Shew_2371 at 2773.900 kb on - strand, within Shew_2371 at 2774.095 kb on + strand, within Shew_2371 at 2774.163 kb on - strand, within Shew_2371 at 2774.542 kb on - strand, within Shew_2372 at 2774.731 kb on - strand at 2774.772 kb on + strand at 2774.984 kb on + strand at 2775.213 kb on - strand, within Shew_2373 at 2775.316 kb on - strand, within Shew_2373
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.12 mg/ml remove 2,771,751 - +1.2 2,772,566 + -0.2 2,772,703 - +0.4 2,772,854 + -0.1 2,773,707 + Shew_2371 0.57 -1.2 2,773,900 - Shew_2371 0.69 +0.3 2,774,095 + Shew_2371 0.80 -0.3 2,774,163 - Shew_2371 0.84 +0.1 2,774,542 - Shew_2372 0.35 +1.2 2,774,731 - +1.0 2,774,772 + +0.8 2,774,984 + -1.6 2,775,213 - Shew_2373 0.31 +0.8 2,775,316 - Shew_2373 0.48 +0.6
Or see this region's nucleotide sequence