Experiment: Phosphomycin 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2102 and def are separated by 301 nucleotides def and Shew_2104 are separated by 179 nucleotides Shew_2104 and Shew_2105 are separated by 379 nucleotides Shew_2105 and Shew_2106 are separated by 48 nucleotides
Shew_2102: Shew_2102 - hypothetical protein (RefSeq), at 2,433,502 to 2,434,332
_2102
Shew_2103: def - peptide deformylase (RefSeq), at 2,434,634 to 2,435,125
def
Shew_2104: Shew_2104 - major facilitator transporter (RefSeq), at 2,435,305 to 2,437,026
_2104
Shew_2105: Shew_2105 - methylated-DNA-(protein)-cysteine S-methyltransferase (RefSeq), at 2,437,406 to 2,437,756
_2105
Shew_2106: Shew_2106 - hypothetical protein (RefSeq), at 2,437,805 to 2,438,596
_2106
Position (kb)
2435
2436
2437
2438 Strain fitness (log2 ratio)
-1
0
1 at 2434.794 kb on + strand, within def at 2434.800 kb on + strand, within def at 2434.975 kb on + strand, within def at 2435.331 kb on + strand at 2435.334 kb on - strand at 2435.339 kb on - strand at 2435.428 kb on + strand at 2435.591 kb on + strand, within Shew_2104 at 2435.710 kb on - strand, within Shew_2104 at 2436.036 kb on + strand, within Shew_2104 at 2436.036 kb on + strand, within Shew_2104 at 2436.124 kb on - strand, within Shew_2104 at 2436.430 kb on - strand, within Shew_2104 at 2436.461 kb on + strand, within Shew_2104 at 2436.469 kb on - strand, within Shew_2104 at 2436.521 kb on - strand, within Shew_2104 at 2436.906 kb on - strand at 2436.906 kb on - strand at 2436.947 kb on + strand at 2437.697 kb on - strand, within Shew_2105 at 2437.750 kb on - strand at 2437.832 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.12 mg/ml remove 2,434,794 + def Shew_2103 0.33 +0.5 2,434,800 + def Shew_2103 0.34 -1.0 2,434,975 + def Shew_2103 0.69 -0.8 2,435,331 + +0.1 2,435,334 - +0.3 2,435,339 - +0.7 2,435,428 + +0.9 2,435,591 + Shew_2104 0.17 -0.5 2,435,710 - Shew_2104 0.24 +0.5 2,436,036 + Shew_2104 0.42 -0.6 2,436,036 + Shew_2104 0.42 +1.0 2,436,124 - Shew_2104 0.48 -0.6 2,436,430 - Shew_2104 0.65 +0.4 2,436,461 + Shew_2104 0.67 +1.1 2,436,469 - Shew_2104 0.68 +0.4 2,436,521 - Shew_2104 0.71 +0.5 2,436,906 - +0.1 2,436,906 - -0.3 2,436,947 + +0.2 2,437,697 - Shew_2105 0.83 -0.4 2,437,750 - -0.3 2,437,832 - -0.5
Or see this region's nucleotide sequence