Experiment: Phosphomycin 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0541 and Shew_0542 are separated by 12 nucleotides Shew_0542 and Shew_0543 are separated by 4 nucleotides Shew_0543 and tolC are separated by 67 nucleotides
Shew_0541: Shew_0541 - hypothetical protein (RefSeq), at 612,211 to 612,678
_0541
Shew_0542: Shew_0542 - hypothetical protein (RefSeq), at 612,691 to 613,662
_0542
Shew_0543: Shew_0543 - hypothetical protein (RefSeq), at 613,667 to 614,425
_0543
Shew_0544: tolC - outer membrane channel protein (RefSeq), at 614,493 to 615,839
tolC
Position (kb) 613
614
615 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 612.689 kb on - strand at 612.704 kb on - strand at 612.785 kb on + strand at 612.785 kb on + strand at 612.819 kb on + strand, within Shew_0542 at 613.228 kb on + strand, within Shew_0542 at 613.295 kb on - strand, within Shew_0542 at 613.425 kb on - strand, within Shew_0542 at 613.490 kb on - strand, within Shew_0542 at 613.564 kb on - strand, within Shew_0542 at 613.581 kb on - strand at 613.702 kb on - strand at 614.025 kb on + strand, within Shew_0543 at 614.249 kb on - strand, within Shew_0543 at 614.414 kb on - strand at 614.637 kb on - strand, within tolC at 614.676 kb on + strand, within tolC at 615.022 kb on + strand, within tolC
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.12 mg/ml remove 612,689 - +0.4 612,704 - +0.4 612,785 + +0.5 612,785 + +0.5 612,819 + Shew_0542 0.13 +0.3 613,228 + Shew_0542 0.55 +0.2 613,295 - Shew_0542 0.62 -0.5 613,425 - Shew_0542 0.76 +0.5 613,490 - Shew_0542 0.82 -0.1 613,564 - Shew_0542 0.90 +0.4 613,581 - +0.5 613,702 - +0.7 614,025 + Shew_0543 0.47 -0.3 614,249 - Shew_0543 0.77 -1.2 614,414 - -1.0 614,637 - tolC Shew_0544 0.11 -3.0 614,676 + tolC Shew_0544 0.14 -2.1 615,022 + tolC Shew_0544 0.39 -0.5
Or see this region's nucleotide sequence