Experiment: Phosphomycin 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0477 and Shew_0478 are separated by 619 nucleotides Shew_0478 and Shew_0479 are separated by 2 nucleotides Shew_0479 and Shew_0480 are separated by 69 nucleotides
Shew_0477: Shew_0477 - hypothetical protein (RefSeq), at 534,699 to 535,280
_0477
Shew_0478: Shew_0478 - XRE family transcriptional regulator (RefSeq), at 535,900 to 536,136
_0478
Shew_0479: Shew_0479 - HipA domain-containing protein (RefSeq), at 536,139 to 537,440
_0479
Shew_0480: Shew_0480 - Sel1 domain-containing protein (RefSeq), at 537,510 to 538,568
_0480
Position (kb) 536
537
538 Strain fitness (log2 ratio) -2
-1
0
1
2 at 535.241 kb on + strand at 535.301 kb on + strand at 535.524 kb on + strand at 535.639 kb on - strand at 535.704 kb on + strand at 535.878 kb on - strand at 536.049 kb on - strand, within Shew_0478 at 536.130 kb on + strand at 536.186 kb on + strand at 536.409 kb on - strand, within Shew_0479 at 536.419 kb on - strand, within Shew_0479 at 536.672 kb on - strand, within Shew_0479 at 536.785 kb on - strand, within Shew_0479 at 536.785 kb on - strand, within Shew_0479 at 536.785 kb on - strand, within Shew_0479 at 536.785 kb on - strand, within Shew_0479 at 536.943 kb on + strand, within Shew_0479 at 537.104 kb on + strand, within Shew_0479 at 537.434 kb on + strand at 537.443 kb on - strand at 537.961 kb on - strand, within Shew_0480 at 538.003 kb on + strand, within Shew_0480
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.12 mg/ml remove 535,241 + +0.1 535,301 + +0.2 535,524 + -0.7 535,639 - -1.0 535,704 + +0.4 535,878 - +0.4 536,049 - Shew_0478 0.63 -0.5 536,130 + +0.8 536,186 + +0.0 536,409 - Shew_0479 0.21 +1.0 536,419 - Shew_0479 0.22 -2.1 536,672 - Shew_0479 0.41 +0.6 536,785 - Shew_0479 0.50 -0.2 536,785 - Shew_0479 0.50 -0.0 536,785 - Shew_0479 0.50 +0.3 536,785 - Shew_0479 0.50 +0.0 536,943 + Shew_0479 0.62 +2.4 537,104 + Shew_0479 0.74 +1.7 537,434 + +0.9 537,443 - -0.0 537,961 - Shew_0480 0.43 +0.7 538,003 + Shew_0480 0.47 +0.4
Or see this region's nucleotide sequence