Experiment: Phosphomycin 0.12 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0238 and Shew_0239 are separated by 138 nucleotides Shew_0239 and Shew_0240 are separated by 64 nucleotides Shew_0240 and Shew_0241 overlap by 1 nucleotides
Shew_0238: Shew_0238 - porin (RefSeq), at 254,419 to 255,507
_0238
Shew_0239: Shew_0239 - formate dehydrogenase, gamma subunit (RefSeq), at 255,646 to 256,626
_0239
Shew_0240: Shew_0240 - cytoplasmic chaperone TorD family protein (RefSeq), at 256,691 to 257,305
_0240
Shew_0241: Shew_0241 - 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein (RefSeq), at 257,305 to 257,850
_0241
Position (kb)
255
256
257 Strain fitness (log2 ratio)
-1
0
1 at 254.661 kb on - strand, within Shew_0238 at 254.778 kb on - strand, within Shew_0238 at 254.782 kb on + strand, within Shew_0238 at 254.825 kb on - strand, within Shew_0238 at 254.859 kb on + strand, within Shew_0238 at 254.869 kb on + strand, within Shew_0238 at 255.431 kb on + strand at 255.436 kb on + strand at 255.581 kb on - strand at 255.727 kb on + strand at 255.791 kb on + strand, within Shew_0239 at 255.905 kb on + strand, within Shew_0239 at 255.924 kb on + strand, within Shew_0239 at 256.098 kb on - strand, within Shew_0239 at 256.169 kb on + strand, within Shew_0239 at 256.379 kb on - strand, within Shew_0239 at 256.563 kb on + strand at 256.571 kb on - strand at 257.060 kb on + strand, within Shew_0240 at 257.281 kb on + strand at 257.567 kb on - strand, within Shew_0241 at 257.567 kb on - strand, within Shew_0241 at 257.584 kb on + strand, within Shew_0241
Per-strain Table
Position Strand Gene LocusTag Fraction Phosphomycin 0.12 mg/ml remove 254,661 - Shew_0238 0.22 -0.7 254,778 - Shew_0238 0.33 +0.6 254,782 + Shew_0238 0.33 +0.1 254,825 - Shew_0238 0.37 -0.5 254,859 + Shew_0238 0.40 -0.4 254,869 + Shew_0238 0.41 +1.4 255,431 + +0.4 255,436 + -0.8 255,581 - -0.4 255,727 + +0.7 255,791 + Shew_0239 0.15 +0.6 255,905 + Shew_0239 0.26 +1.2 255,924 + Shew_0239 0.28 +0.2 256,098 - Shew_0239 0.46 -0.1 256,169 + Shew_0239 0.53 +0.2 256,379 - Shew_0239 0.75 +0.2 256,563 + +0.1 256,571 - +0.5 257,060 + Shew_0240 0.60 +0.3 257,281 + -1.5 257,567 - Shew_0241 0.48 +0.7 257,567 - Shew_0241 0.48 +0.1 257,584 + Shew_0241 0.51 +0.5
Or see this region's nucleotide sequence