Experiment: Succinic Acid; Light intensity 18 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0047 and Rru_A0048 are separated by 142 nucleotides Rru_A0048 and Rru_A0049 are separated by 78 nucleotides Rru_A0049 and Rru_A0050 overlap by 4 nucleotides Rru_A0050 and Rru_A0051 are separated by 12 nucleotides
Rru_A0047: Rru_A0047 - ABC transporter component (NCBI), at 48,770 to 50,377
_A0047
Rru_A0048: Rru_A0048 - Lysine exporter protein (LYSE/YGGA) (NCBI), at 50,520 to 51,146
_A0048
Rru_A0049: Rru_A0049 - Biotin/lipoyl attachment (NCBI), at 51,225 to 51,629
_A0049
Rru_A0050: Rru_A0050 - hypothetical protein (NCBI), at 51,626 to 52,033
_A0050
Rru_A0051: Rru_A0051 - Carboxyl transferase (NCBI), at 52,046 to 53,599
_A0051
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 49.722 kb on + strand, within Rru_A0047 at 49.723 kb on - strand, within Rru_A0047 at 49.734 kb on + strand, within Rru_A0047 at 49.734 kb on + strand, within Rru_A0047 at 49.837 kb on + strand, within Rru_A0047 at 49.837 kb on + strand, within Rru_A0047 at 49.837 kb on + strand, within Rru_A0047 at 49.838 kb on - strand, within Rru_A0047 at 49.838 kb on - strand, within Rru_A0047 at 49.838 kb on - strand, within Rru_A0047 at 49.838 kb on - strand, within Rru_A0047 at 49.876 kb on + strand, within Rru_A0047 at 49.876 kb on + strand, within Rru_A0047 at 49.877 kb on - strand, within Rru_A0047 at 49.877 kb on - strand, within Rru_A0047 at 49.877 kb on - strand, within Rru_A0047 at 49.877 kb on - strand, within Rru_A0047 at 50.300 kb on - strand at 50.478 kb on - strand at 50.478 kb on - strand at 50.521 kb on + strand at 50.522 kb on - strand at 50.522 kb on - strand at 50.522 kb on - strand at 50.614 kb on + strand, within Rru_A0048 at 50.615 kb on - strand, within Rru_A0048 at 50.615 kb on - strand, within Rru_A0048 at 50.654 kb on - strand, within Rru_A0048 at 50.964 kb on + strand, within Rru_A0048 at 50.965 kb on - strand, within Rru_A0048 at 50.965 kb on - strand, within Rru_A0048 at 51.077 kb on + strand, within Rru_A0048 at 51.077 kb on + strand, within Rru_A0048 at 51.077 kb on + strand, within Rru_A0048 at 51.077 kb on + strand, within Rru_A0048 at 51.077 kb on + strand, within Rru_A0048 at 51.077 kb on + strand, within Rru_A0048 at 51.078 kb on - strand, within Rru_A0048 at 51.078 kb on - strand, within Rru_A0048 at 51.646 kb on + strand at 51.754 kb on + strand, within Rru_A0050 at 51.755 kb on - strand, within Rru_A0050 at 51.755 kb on - strand, within Rru_A0050 at 51.781 kb on + strand, within Rru_A0050 at 51.781 kb on + strand, within Rru_A0050 at 51.781 kb on + strand, within Rru_A0050 at 51.782 kb on - strand, within Rru_A0050 at 51.786 kb on + strand, within Rru_A0050 at 52.047 kb on + strand at 52.047 kb on + strand at 52.047 kb on + strand at 52.047 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 18 uM remove 49,722 + Rru_A0047 0.59 +2.7 49,723 - Rru_A0047 0.59 -1.8 49,734 + Rru_A0047 0.60 -1.8 49,734 + Rru_A0047 0.60 -1.0 49,837 + Rru_A0047 0.66 +2.4 49,837 + Rru_A0047 0.66 -0.5 49,837 + Rru_A0047 0.66 -0.4 49,838 - Rru_A0047 0.66 +2.8 49,838 - Rru_A0047 0.66 -0.4 49,838 - Rru_A0047 0.66 -0.3 49,838 - Rru_A0047 0.66 -0.0 49,876 + Rru_A0047 0.69 -1.1 49,876 + Rru_A0047 0.69 -1.7 49,877 - Rru_A0047 0.69 -0.0 49,877 - Rru_A0047 0.69 +0.7 49,877 - Rru_A0047 0.69 +1.6 49,877 - Rru_A0047 0.69 +0.8 50,300 - +5.3 50,478 - +4.1 50,478 - -0.0 50,521 + -1.1 50,522 - -0.0 50,522 - -0.8 50,522 - -0.4 50,614 + Rru_A0048 0.15 -0.0 50,615 - Rru_A0048 0.15 -1.0 50,615 - Rru_A0048 0.15 -0.0 50,654 - Rru_A0048 0.21 -0.5 50,964 + Rru_A0048 0.71 +4.6 50,965 - Rru_A0048 0.71 +0.5 50,965 - Rru_A0048 0.71 +1.7 51,077 + Rru_A0048 0.89 -0.0 51,077 + Rru_A0048 0.89 +0.2 51,077 + Rru_A0048 0.89 +5.9 51,077 + Rru_A0048 0.89 +1.6 51,077 + Rru_A0048 0.89 +0.2 51,077 + Rru_A0048 0.89 -0.3 51,078 - Rru_A0048 0.89 -0.0 51,078 - Rru_A0048 0.89 -0.8 51,646 + -0.0 51,754 + Rru_A0050 0.31 -0.0 51,755 - Rru_A0050 0.32 -0.5 51,755 - Rru_A0050 0.32 -0.0 51,781 + Rru_A0050 0.38 -0.0 51,781 + Rru_A0050 0.38 -0.0 51,781 + Rru_A0050 0.38 +1.3 51,782 - Rru_A0050 0.38 -0.8 51,786 + Rru_A0050 0.39 -0.0 52,047 + +0.4 52,047 + -0.4 52,047 + -0.0 52,047 + +1.9
Or see this region's nucleotide sequence