Experiment: Succinic Acid; Light intensity 18 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_B0010 and Rru_B0011 are separated by 12 nucleotides Rru_B0011 and Rru_B0012 are separated by 60 nucleotides Rru_B0012 and Rru_B0013 are separated by 183 nucleotides Rru_B0013 and Rru_B0014 are separated by 0 nucleotides Rru_B0014 and Rru_B0015 are separated by 27 nucleotides
Rru_B0010: Rru_B0010 - Resolvase (NCBI), at 12,479 to 13,036
_B0010
Rru_B0011: Rru_B0011 - Protein of unknown function DUF86 (NCBI), at 13,049 to 13,348
_B0011
Rru_B0012: Rru_B0012 - DNA polymerase, beta-like region (NCBI), at 13,409 to 13,675
_B0012
Rru_B0013: Rru_B0013 - hypothetical protein (NCBI), at 13,859 to 14,095
_B0013
Rru_B0014: Rru_B0014 - Transposase, mutator type (NCBI), at 14,096 to 15,022
_B0014
Rru_B0015: Rru_B0015 - putative fragment of transposase protein (NCBI), at 15,050 to 15,301
_B0015
Position (kb)
13
14
15 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7 at 12.881 kb on + strand, within Rru_B0010 at 12.882 kb on - strand, within Rru_B0010 at 12.882 kb on - strand, within Rru_B0010 at 12.882 kb on - strand, within Rru_B0010 at 12.882 kb on - strand, within Rru_B0010 at 12.937 kb on + strand, within Rru_B0010 at 13.164 kb on + strand, within Rru_B0011 at 13.164 kb on + strand, within Rru_B0011 at 13.164 kb on + strand, within Rru_B0011 at 13.164 kb on + strand, within Rru_B0011 at 13.165 kb on - strand, within Rru_B0011 at 13.183 kb on + strand, within Rru_B0011 at 13.184 kb on - strand, within Rru_B0011 at 13.184 kb on - strand, within Rru_B0011 at 13.242 kb on + strand, within Rru_B0011 at 13.243 kb on - strand, within Rru_B0011 at 13.288 kb on + strand, within Rru_B0011 at 13.288 kb on + strand, within Rru_B0011 at 13.288 kb on + strand, within Rru_B0011 at 13.288 kb on + strand, within Rru_B0011 at 13.288 kb on + strand, within Rru_B0011 at 13.288 kb on + strand, within Rru_B0011 at 13.289 kb on - strand, within Rru_B0011 at 13.289 kb on - strand, within Rru_B0011 at 13.289 kb on - strand, within Rru_B0011 at 13.292 kb on - strand, within Rru_B0011 at 13.292 kb on - strand, within Rru_B0011 at 13.292 kb on - strand, within Rru_B0011 at 13.292 kb on - strand, within Rru_B0011 at 13.338 kb on - strand at 13.392 kb on + strand at 13.392 kb on + strand at 13.392 kb on + strand at 13.393 kb on - strand at 13.412 kb on + strand at 13.412 kb on + strand at 13.412 kb on + strand at 13.412 kb on + strand at 13.412 kb on + strand at 13.413 kb on - strand at 13.413 kb on - strand at 13.413 kb on - strand at 13.413 kb on - strand at 13.413 kb on - strand at 13.540 kb on + strand, within Rru_B0012 at 13.540 kb on + strand, within Rru_B0012 at 13.540 kb on + strand, within Rru_B0012 at 13.540 kb on + strand, within Rru_B0012 at 13.540 kb on + strand, within Rru_B0012 at 13.540 kb on + strand, within Rru_B0012 at 13.540 kb on + strand, within Rru_B0012 at 13.541 kb on - strand, within Rru_B0012 at 13.541 kb on - strand, within Rru_B0012 at 13.541 kb on - strand, within Rru_B0012 at 13.541 kb on - strand, within Rru_B0012 at 13.541 kb on - strand, within Rru_B0012 at 13.541 kb on - strand, within Rru_B0012 at 13.541 kb on - strand, within Rru_B0012 at 13.647 kb on - strand, within Rru_B0012 at 13.687 kb on + strand at 13.687 kb on + strand at 13.688 kb on - strand at 13.688 kb on - strand at 13.688 kb on - strand at 13.688 kb on - strand at 13.688 kb on - strand at 13.688 kb on - strand at 13.688 kb on - strand at 13.688 kb on - strand at 13.688 kb on - strand at 13.714 kb on - strand at 13.735 kb on - strand at 13.735 kb on - strand at 13.763 kb on + strand at 13.763 kb on + strand at 13.764 kb on - strand at 13.764 kb on - strand at 13.848 kb on + strand at 14.050 kb on - strand, within Rru_B0013 at 14.050 kb on - strand, within Rru_B0013 at 14.050 kb on - strand, within Rru_B0013 at 14.050 kb on - strand, within Rru_B0013
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 18 uM remove 12,881 + Rru_B0010 0.72 +1.2 12,882 - Rru_B0010 0.72 +0.2 12,882 - Rru_B0010 0.72 -1.2 12,882 - Rru_B0010 0.72 -1.2 12,882 - Rru_B0010 0.72 +0.2 12,937 + Rru_B0010 0.82 -1.0 13,164 + Rru_B0011 0.38 +2.8 13,164 + Rru_B0011 0.38 -0.7 13,164 + Rru_B0011 0.38 -1.2 13,164 + Rru_B0011 0.38 -0.8 13,165 - Rru_B0011 0.39 +1.3 13,183 + Rru_B0011 0.45 +0.4 13,184 - Rru_B0011 0.45 +4.1 13,184 - Rru_B0011 0.45 -0.6 13,242 + Rru_B0011 0.64 -0.8 13,243 - Rru_B0011 0.65 -0.8 13,288 + Rru_B0011 0.80 +0.2 13,288 + Rru_B0011 0.80 -0.8 13,288 + Rru_B0011 0.80 +0.9 13,288 + Rru_B0011 0.80 +1.1 13,288 + Rru_B0011 0.80 +0.2 13,288 + Rru_B0011 0.80 +1.2 13,289 - Rru_B0011 0.80 -2.0 13,289 - Rru_B0011 0.80 -0.4 13,289 - Rru_B0011 0.80 +2.2 13,292 - Rru_B0011 0.81 +0.2 13,292 - Rru_B0011 0.81 -0.8 13,292 - Rru_B0011 0.81 -3.5 13,292 - Rru_B0011 0.81 -1.6 13,338 - +0.0 13,392 + +2.2 13,392 + +0.2 13,392 + +0.2 13,393 - -0.3 13,412 + -0.1 13,412 + +0.2 13,412 + -1.4 13,412 + -0.3 13,412 + +2.1 13,413 - +0.2 13,413 - +0.2 13,413 - -1.8 13,413 - +0.2 13,413 - -0.6 13,540 + Rru_B0012 0.49 -1.2 13,540 + Rru_B0012 0.49 +2.3 13,540 + Rru_B0012 0.49 +0.2 13,540 + Rru_B0012 0.49 -1.4 13,540 + Rru_B0012 0.49 +0.2 13,540 + Rru_B0012 0.49 +6.8 13,540 + Rru_B0012 0.49 -1.1 13,541 - Rru_B0012 0.49 +1.5 13,541 - Rru_B0012 0.49 -2.6 13,541 - Rru_B0012 0.49 +0.2 13,541 - Rru_B0012 0.49 -1.4 13,541 - Rru_B0012 0.49 -1.0 13,541 - Rru_B0012 0.49 +0.3 13,541 - Rru_B0012 0.49 -0.9 13,647 - Rru_B0012 0.89 -0.8 13,687 + +0.2 13,687 + +0.2 13,688 - -1.1 13,688 - +4.5 13,688 - -0.9 13,688 - +0.2 13,688 - +0.2 13,688 - -0.9 13,688 - -0.5 13,688 - +3.2 13,688 - +5.1 13,714 - +0.2 13,735 - +6.0 13,735 - -0.8 13,763 + -0.6 13,763 + +0.0 13,764 - +1.4 13,764 - +0.1 13,848 + -0.9 14,050 - Rru_B0013 0.81 +0.2 14,050 - Rru_B0013 0.81 -0.2 14,050 - Rru_B0013 0.81 +0.2 14,050 - Rru_B0013 0.81 -1.5
Or see this region's nucleotide sequence