Experiment: Hans 1000x minerals
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_433 and Pf6N2E2_434 overlap by 4 nucleotides Pf6N2E2_434 and Pf6N2E2_435 are separated by 15 nucleotides Pf6N2E2_435 and Pf6N2E2_436 overlap by 37 nucleotides
Pf6N2E2_433 - Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9), at 500,337 to 501,410
_433
Pf6N2E2_434 - hypothetical protein, at 501,407 to 501,544
_434
Pf6N2E2_435 - Probable transcription regulator, at 501,560 to 502,048
_435
Pf6N2E2_436 - ADA regulatory protein / Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 502,012 to 503,085
_436
Position (kb) 501
502
503 Strain fitness (log2 ratio) -2
-1
0
1 at 500.677 kb on + strand, within Pf6N2E2_433 at 500.870 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433 at 501.122 kb on - strand, within Pf6N2E2_433 at 501.448 kb on + strand, within Pf6N2E2_434 at 501.448 kb on + strand, within Pf6N2E2_434 at 501.525 kb on - strand, within Pf6N2E2_434 at 501.540 kb on + strand at 501.541 kb on - strand at 501.541 kb on - strand at 501.743 kb on + strand, within Pf6N2E2_435 at 501.744 kb on - strand, within Pf6N2E2_435 at 501.744 kb on - strand, within Pf6N2E2_435 at 501.744 kb on - strand, within Pf6N2E2_435 at 501.753 kb on - strand, within Pf6N2E2_435 at 501.851 kb on + strand, within Pf6N2E2_435 at 501.851 kb on + strand, within Pf6N2E2_435 at 501.920 kb on + strand, within Pf6N2E2_435 at 501.921 kb on - strand, within Pf6N2E2_435 at 501.975 kb on - strand, within Pf6N2E2_435 at 502.001 kb on + strand at 502.001 kb on + strand at 502.002 kb on - strand at 502.002 kb on - strand at 502.002 kb on - strand at 502.099 kb on + strand at 502.100 kb on - strand at 502.206 kb on - strand, within Pf6N2E2_436 at 502.300 kb on + strand, within Pf6N2E2_436 at 502.301 kb on - strand, within Pf6N2E2_436 at 502.301 kb on - strand, within Pf6N2E2_436 at 502.577 kb on + strand, within Pf6N2E2_436 at 502.578 kb on - strand, within Pf6N2E2_436 at 502.578 kb on - strand, within Pf6N2E2_436 at 502.578 kb on - strand, within Pf6N2E2_436 at 502.610 kb on + strand, within Pf6N2E2_436 at 502.610 kb on + strand, within Pf6N2E2_436 at 502.610 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.967 kb on + strand, within Pf6N2E2_436 at 502.968 kb on - strand, within Pf6N2E2_436 at 502.968 kb on - strand, within Pf6N2E2_436 at 502.968 kb on - strand, within Pf6N2E2_436 at 502.984 kb on + strand at 502.991 kb on + strand at 502.991 kb on + strand at 502.992 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 1000x minerals remove 500,677 + Pf6N2E2_433 0.32 +0.6 500,870 - Pf6N2E2_433 0.50 +0.7 501,122 - Pf6N2E2_433 0.73 +1.2 501,122 - Pf6N2E2_433 0.73 -0.2 501,122 - Pf6N2E2_433 0.73 +0.5 501,122 - Pf6N2E2_433 0.73 -0.2 501,448 + Pf6N2E2_434 0.30 +0.2 501,448 + Pf6N2E2_434 0.30 +1.4 501,525 - Pf6N2E2_434 0.86 +0.3 501,540 + -0.2 501,541 - +1.5 501,541 - -1.1 501,743 + Pf6N2E2_435 0.37 -0.5 501,744 - Pf6N2E2_435 0.38 -2.1 501,744 - Pf6N2E2_435 0.38 +0.4 501,744 - Pf6N2E2_435 0.38 -0.7 501,753 - Pf6N2E2_435 0.39 -0.2 501,851 + Pf6N2E2_435 0.60 -0.9 501,851 + Pf6N2E2_435 0.60 -1.2 501,920 + Pf6N2E2_435 0.74 -0.8 501,921 - Pf6N2E2_435 0.74 -0.3 501,975 - Pf6N2E2_435 0.85 +0.7 502,001 + +0.2 502,001 + +0.6 502,002 - -0.5 502,002 - -0.8 502,002 - +0.8 502,099 + -0.3 502,100 - -0.5 502,206 - Pf6N2E2_436 0.18 +0.8 502,300 + Pf6N2E2_436 0.27 -1.2 502,301 - Pf6N2E2_436 0.27 +0.9 502,301 - Pf6N2E2_436 0.27 -0.4 502,577 + Pf6N2E2_436 0.53 +0.8 502,578 - Pf6N2E2_436 0.53 +1.5 502,578 - Pf6N2E2_436 0.53 +1.0 502,578 - Pf6N2E2_436 0.53 +0.7 502,610 + Pf6N2E2_436 0.56 +0.3 502,610 + Pf6N2E2_436 0.56 -0.2 502,610 + Pf6N2E2_436 0.56 +0.0 502,967 + Pf6N2E2_436 0.89 -0.2 502,967 + Pf6N2E2_436 0.89 -0.9 502,967 + Pf6N2E2_436 0.89 -0.6 502,967 + Pf6N2E2_436 0.89 -1.0 502,967 + Pf6N2E2_436 0.89 +0.1 502,967 + Pf6N2E2_436 0.89 -0.1 502,967 + Pf6N2E2_436 0.89 +0.1 502,968 - Pf6N2E2_436 0.89 -0.1 502,968 - Pf6N2E2_436 0.89 -0.4 502,968 - Pf6N2E2_436 0.89 +0.3 502,984 + -1.3 502,991 + +1.4 502,991 + -1.1 502,992 - -0.5
Or see this region's nucleotide sequence