Experiment: Hans 1000x minerals
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1664 and Pf6N2E2_1665 overlap by 6 nucleotides Pf6N2E2_1665 and Pf6N2E2_1666 are separated by 210 nucleotides
Pf6N2E2_1664 - Nitrate/nitrite transporter, at 1,970,587 to 1,971,969
_1664
Pf6N2E2_1665 - hypothetical protein, at 1,971,964 to 1,972,113
_1665
Pf6N2E2_1666 - Transcriptional regulator, LysR family, at 1,972,324 to 1,973,262
_1666
Position (kb)
1971
1972
1973 Strain fitness (log2 ratio)
-2
-1
0
1 at 1971.137 kb on + strand, within Pf6N2E2_1664 at 1971.427 kb on + strand, within Pf6N2E2_1664 at 1971.509 kb on + strand, within Pf6N2E2_1664 at 1971.510 kb on - strand, within Pf6N2E2_1664 at 1971.518 kb on + strand, within Pf6N2E2_1664 at 1971.519 kb on - strand, within Pf6N2E2_1664 at 1971.519 kb on - strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.578 kb on + strand, within Pf6N2E2_1664 at 1971.579 kb on - strand, within Pf6N2E2_1664 at 1971.579 kb on - strand, within Pf6N2E2_1664 at 1971.579 kb on - strand, within Pf6N2E2_1664 at 1971.630 kb on - strand, within Pf6N2E2_1664 at 1971.770 kb on + strand, within Pf6N2E2_1664 at 1971.770 kb on + strand, within Pf6N2E2_1664 at 1971.770 kb on + strand, within Pf6N2E2_1664 at 1971.770 kb on + strand, within Pf6N2E2_1664 at 1971.771 kb on - strand, within Pf6N2E2_1664 at 1971.854 kb on + strand at 1971.854 kb on + strand at 1971.855 kb on - strand at 1971.899 kb on + strand at 1971.900 kb on - strand at 1971.974 kb on + strand at 1971.975 kb on - strand at 1971.975 kb on - strand at 1971.975 kb on - strand at 1972.041 kb on + strand, within Pf6N2E2_1665 at 1972.041 kb on + strand, within Pf6N2E2_1665 at 1972.041 kb on + strand, within Pf6N2E2_1665 at 1972.041 kb on + strand, within Pf6N2E2_1665 at 1972.042 kb on - strand, within Pf6N2E2_1665 at 1972.131 kb on + strand at 1972.131 kb on + strand at 1972.131 kb on + strand at 1972.131 kb on + strand at 1972.206 kb on + strand at 1972.239 kb on + strand at 1972.709 kb on + strand, within Pf6N2E2_1666 at 1972.710 kb on - strand, within Pf6N2E2_1666 at 1972.710 kb on - strand, within Pf6N2E2_1666 at 1972.839 kb on + strand, within Pf6N2E2_1666 at 1973.028 kb on - strand, within Pf6N2E2_1666 at 1973.035 kb on + strand, within Pf6N2E2_1666
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 1000x minerals remove 1,971,137 + Pf6N2E2_1664 0.40 -1.0 1,971,427 + Pf6N2E2_1664 0.61 +1.1 1,971,509 + Pf6N2E2_1664 0.67 +0.1 1,971,510 - Pf6N2E2_1664 0.67 +0.1 1,971,518 + Pf6N2E2_1664 0.67 +0.3 1,971,519 - Pf6N2E2_1664 0.67 -0.9 1,971,519 - Pf6N2E2_1664 0.67 -0.4 1,971,578 + Pf6N2E2_1664 0.72 +0.5 1,971,578 + Pf6N2E2_1664 0.72 +1.1 1,971,578 + Pf6N2E2_1664 0.72 -0.7 1,971,578 + Pf6N2E2_1664 0.72 -0.3 1,971,579 - Pf6N2E2_1664 0.72 +1.1 1,971,579 - Pf6N2E2_1664 0.72 -0.7 1,971,579 - Pf6N2E2_1664 0.72 +0.1 1,971,630 - Pf6N2E2_1664 0.75 +1.4 1,971,770 + Pf6N2E2_1664 0.86 -1.5 1,971,770 + Pf6N2E2_1664 0.86 +0.5 1,971,770 + Pf6N2E2_1664 0.86 -0.7 1,971,770 + Pf6N2E2_1664 0.86 +0.3 1,971,771 - Pf6N2E2_1664 0.86 -0.0 1,971,854 + -0.3 1,971,854 + +0.2 1,971,855 - -0.6 1,971,899 + +0.4 1,971,900 - -0.2 1,971,974 + -1.6 1,971,975 - +0.1 1,971,975 - +1.1 1,971,975 - +1.1 1,972,041 + Pf6N2E2_1665 0.51 -1.4 1,972,041 + Pf6N2E2_1665 0.51 +0.3 1,972,041 + Pf6N2E2_1665 0.51 -0.4 1,972,041 + Pf6N2E2_1665 0.51 -1.8 1,972,042 - Pf6N2E2_1665 0.52 -0.2 1,972,131 + -0.2 1,972,131 + +0.2 1,972,131 + +0.5 1,972,131 + +0.7 1,972,206 + +0.7 1,972,239 + -1.6 1,972,709 + Pf6N2E2_1666 0.41 +0.2 1,972,710 - Pf6N2E2_1666 0.41 -0.5 1,972,710 - Pf6N2E2_1666 0.41 +0.9 1,972,839 + Pf6N2E2_1666 0.55 +0.3 1,973,028 - Pf6N2E2_1666 0.75 +0.5 1,973,035 + Pf6N2E2_1666 0.76 +0.7
Or see this region's nucleotide sequence