Experiment: Hans 1000x minerals
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1427 and Pf6N2E2_1428 are separated by 305 nucleotides Pf6N2E2_1428 and Pf6N2E2_1429 are separated by 133 nucleotides Pf6N2E2_1429 and Pf6N2E2_1430 are separated by 56 nucleotides
Pf6N2E2_1427 - 6-aminohexanoate-dimer hydrolase (EC 3.5.1.46), at 1,675,677 to 1,676,939
_1427
Pf6N2E2_1428 - Alkanesulfonates-binding protein, at 1,677,245 to 1,678,348
_1428
Pf6N2E2_1429 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 1,678,482 to 1,679,243
_1429
Pf6N2E2_1430 - Leucine-, isoleucine-, valine-, threonine-, and alanine-binding protein, at 1,679,300 to 1,680,625
_1430
Position (kb)
1677
1678
1679 Strain fitness (log2 ratio)
-1
0
1 at 1676.260 kb on + strand, within Pf6N2E2_1427 at 1676.261 kb on - strand, within Pf6N2E2_1427 at 1676.440 kb on + strand, within Pf6N2E2_1427 at 1676.440 kb on + strand, within Pf6N2E2_1427 at 1676.440 kb on + strand, within Pf6N2E2_1427 at 1676.441 kb on - strand, within Pf6N2E2_1427 at 1676.441 kb on - strand, within Pf6N2E2_1427 at 1677.202 kb on + strand at 1677.255 kb on + strand at 1677.430 kb on - strand, within Pf6N2E2_1428 at 1677.430 kb on - strand, within Pf6N2E2_1428 at 1677.430 kb on - strand, within Pf6N2E2_1428 at 1677.430 kb on - strand, within Pf6N2E2_1428 at 1677.708 kb on + strand, within Pf6N2E2_1428 at 1677.708 kb on + strand, within Pf6N2E2_1428 at 1677.709 kb on - strand, within Pf6N2E2_1428 at 1677.709 kb on - strand, within Pf6N2E2_1428 at 1677.709 kb on - strand, within Pf6N2E2_1428 at 1677.709 kb on - strand, within Pf6N2E2_1428 at 1677.732 kb on + strand, within Pf6N2E2_1428 at 1677.732 kb on + strand, within Pf6N2E2_1428 at 1677.732 kb on + strand, within Pf6N2E2_1428 at 1677.733 kb on - strand, within Pf6N2E2_1428 at 1677.733 kb on - strand, within Pf6N2E2_1428 at 1677.733 kb on - strand, within Pf6N2E2_1428 at 1677.780 kb on - strand, within Pf6N2E2_1428 at 1677.780 kb on - strand, within Pf6N2E2_1428 at 1677.853 kb on - strand, within Pf6N2E2_1428 at 1677.873 kb on - strand, within Pf6N2E2_1428 at 1678.152 kb on - strand, within Pf6N2E2_1428 at 1678.152 kb on - strand, within Pf6N2E2_1428 at 1678.176 kb on + strand, within Pf6N2E2_1428 at 1678.177 kb on - strand, within Pf6N2E2_1428 at 1678.395 kb on + strand at 1678.440 kb on + strand at 1678.441 kb on - strand at 1678.441 kb on - strand at 1678.441 kb on - strand at 1678.588 kb on + strand, within Pf6N2E2_1429 at 1678.588 kb on + strand, within Pf6N2E2_1429 at 1678.588 kb on + strand, within Pf6N2E2_1429 at 1678.588 kb on + strand, within Pf6N2E2_1429 at 1678.589 kb on - strand, within Pf6N2E2_1429 at 1678.589 kb on - strand, within Pf6N2E2_1429 at 1678.589 kb on - strand, within Pf6N2E2_1429 at 1678.589 kb on - strand, within Pf6N2E2_1429 at 1678.589 kb on - strand, within Pf6N2E2_1429 at 1678.589 kb on - strand, within Pf6N2E2_1429 at 1679.106 kb on - strand, within Pf6N2E2_1429 at 1679.195 kb on - strand at 1679.263 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Hans 1000x minerals remove 1,676,260 + Pf6N2E2_1427 0.46 -0.0 1,676,261 - Pf6N2E2_1427 0.46 -1.5 1,676,440 + Pf6N2E2_1427 0.60 +1.2 1,676,440 + Pf6N2E2_1427 0.60 +0.4 1,676,440 + Pf6N2E2_1427 0.60 +1.0 1,676,441 - Pf6N2E2_1427 0.60 +0.5 1,676,441 - Pf6N2E2_1427 0.60 +0.7 1,677,202 + -0.8 1,677,255 + +0.7 1,677,430 - Pf6N2E2_1428 0.17 -0.3 1,677,430 - Pf6N2E2_1428 0.17 -1.1 1,677,430 - Pf6N2E2_1428 0.17 -1.1 1,677,430 - Pf6N2E2_1428 0.17 -1.0 1,677,708 + Pf6N2E2_1428 0.42 +0.8 1,677,708 + Pf6N2E2_1428 0.42 -0.8 1,677,709 - Pf6N2E2_1428 0.42 +0.4 1,677,709 - Pf6N2E2_1428 0.42 +0.3 1,677,709 - Pf6N2E2_1428 0.42 -1.4 1,677,709 - Pf6N2E2_1428 0.42 -0.5 1,677,732 + Pf6N2E2_1428 0.44 -0.6 1,677,732 + Pf6N2E2_1428 0.44 -1.4 1,677,732 + Pf6N2E2_1428 0.44 -1.4 1,677,733 - Pf6N2E2_1428 0.44 -0.1 1,677,733 - Pf6N2E2_1428 0.44 -0.0 1,677,733 - Pf6N2E2_1428 0.44 -0.2 1,677,780 - Pf6N2E2_1428 0.48 -0.6 1,677,780 - Pf6N2E2_1428 0.48 -0.8 1,677,853 - Pf6N2E2_1428 0.55 +1.0 1,677,873 - Pf6N2E2_1428 0.57 +0.2 1,678,152 - Pf6N2E2_1428 0.82 -0.6 1,678,152 - Pf6N2E2_1428 0.82 +0.7 1,678,176 + Pf6N2E2_1428 0.84 -0.5 1,678,177 - Pf6N2E2_1428 0.84 -0.9 1,678,395 + -0.7 1,678,440 + -1.5 1,678,441 - +0.1 1,678,441 - +0.9 1,678,441 - +0.6 1,678,588 + Pf6N2E2_1429 0.14 +0.4 1,678,588 + Pf6N2E2_1429 0.14 -0.2 1,678,588 + Pf6N2E2_1429 0.14 +0.7 1,678,588 + Pf6N2E2_1429 0.14 -0.2 1,678,589 - Pf6N2E2_1429 0.14 -0.6 1,678,589 - Pf6N2E2_1429 0.14 +0.4 1,678,589 - Pf6N2E2_1429 0.14 -1.6 1,678,589 - Pf6N2E2_1429 0.14 +0.4 1,678,589 - Pf6N2E2_1429 0.14 +0.0 1,678,589 - Pf6N2E2_1429 0.14 -0.4 1,679,106 - Pf6N2E2_1429 0.82 -0.5 1,679,195 - +0.2 1,679,263 + -0.0
Or see this region's nucleotide sequence