Experiment: L-Glutamic (C) and 0.25mM L-Methionine; no added vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ga0059261_2191 and Ga0059261_2192 are separated by 49 nucleotides Ga0059261_2192 and Ga0059261_2193 are separated by 7 nucleotides Ga0059261_2193 and Ga0059261_2194 are separated by 10 nucleotides Ga0059261_2194 and Ga0059261_2195 overlap by 4 nucleotides
Ga0059261_2191: Ga0059261_2191 - ribosomal subunit interface protein, at 2,299,292 to 2,299,870
_2191
Ga0059261_2192: Ga0059261_2192 - DNA polymerase III, epsilon subunit, Proteobacterial, at 2,299,920 to 2,300,612
_2192
Ga0059261_2193: Ga0059261_2193 - dephospho-CoA kinase, at 2,300,620 to 2,301,213
_2193
Ga0059261_2194: Ga0059261_2194 - shikimate dehydrogenase (EC 1.1.1.25) (from data) , at 2,301,224 to 2,302,036
_2194
Ga0059261_2195: Ga0059261_2195 - Nucleotide-binding protein implicated in inhibition of septum formation, at 2,302,033 to 2,302,620
_2195
Position (kb)
2300
2301
2302 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2299.725 kb on + strand, within Ga0059261_2191 at 2299.725 kb on + strand, within Ga0059261_2191 at 2299.725 kb on + strand, within Ga0059261_2191 at 2299.725 kb on + strand, within Ga0059261_2191 at 2299.725 kb on + strand, within Ga0059261_2191 at 2299.779 kb on + strand, within Ga0059261_2191 at 2299.779 kb on + strand, within Ga0059261_2191 at 2299.779 kb on + strand, within Ga0059261_2191 at 2299.780 kb on - strand, within Ga0059261_2191 at 2299.870 kb on + strand at 2299.870 kb on + strand at 2299.870 kb on + strand at 2299.870 kb on + strand at 2299.870 kb on + strand at 2299.870 kb on + strand at 2299.871 kb on - strand at 2299.871 kb on - strand at 2299.871 kb on - strand at 2299.886 kb on + strand at 2299.886 kb on + strand at 2299.886 kb on + strand at 2299.886 kb on + strand at 2299.886 kb on + strand at 2299.887 kb on - strand at 2299.887 kb on - strand at 2299.899 kb on + strand at 2299.899 kb on + strand at 2299.899 kb on + strand at 2299.899 kb on + strand at 2299.900 kb on - strand at 2299.900 kb on - strand at 2299.900 kb on - strand at 2301.394 kb on - strand, within Ga0059261_2194 at 2301.394 kb on - strand, within Ga0059261_2194 at 2301.394 kb on - strand, within Ga0059261_2194 at 2301.394 kb on - strand, within Ga0059261_2194 at 2301.394 kb on - strand, within Ga0059261_2194 at 2301.406 kb on - strand, within Ga0059261_2194 at 2301.406 kb on - strand, within Ga0059261_2194 at 2301.406 kb on - strand, within Ga0059261_2194 at 2301.406 kb on - strand, within Ga0059261_2194 at 2301.709 kb on - strand, within Ga0059261_2194 at 2301.709 kb on - strand, within Ga0059261_2194 at 2301.727 kb on + strand, within Ga0059261_2194 at 2301.933 kb on + strand, within Ga0059261_2194 at 2301.933 kb on + strand, within Ga0059261_2194 at 2302.024 kb on - strand at 2302.024 kb on - strand at 2302.056 kb on + strand at 2302.056 kb on + strand at 2302.056 kb on + strand at 2302.056 kb on + strand at 2302.056 kb on + strand at 2302.056 kb on + strand at 2302.056 kb on + strand at 2302.057 kb on - strand at 2302.057 kb on - strand at 2302.057 kb on - strand at 2302.057 kb on - strand at 2302.065 kb on - strand at 2302.065 kb on - strand at 2302.065 kb on - strand at 2302.065 kb on - strand at 2302.103 kb on + strand, within Ga0059261_2195 at 2302.103 kb on + strand, within Ga0059261_2195 at 2302.103 kb on + strand, within Ga0059261_2195 at 2302.103 kb on + strand, within Ga0059261_2195 at 2302.104 kb on - strand, within Ga0059261_2195 at 2302.104 kb on - strand, within Ga0059261_2195 at 2302.202 kb on + strand, within Ga0059261_2195 at 2302.202 kb on + strand, within Ga0059261_2195 at 2302.203 kb on - strand, within Ga0059261_2195 at 2302.203 kb on - strand, within Ga0059261_2195 at 2302.203 kb on - strand, within Ga0059261_2195 at 2302.203 kb on - strand, within Ga0059261_2195
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic (C) and 0.25mM L-Methionine; no added vitamins remove 2,299,725 + Ga0059261_2191 0.75 -2.9 2,299,725 + Ga0059261_2191 0.75 -4.0 2,299,725 + Ga0059261_2191 0.75 -2.9 2,299,725 + Ga0059261_2191 0.75 -4.5 2,299,725 + Ga0059261_2191 0.75 -4.1 2,299,779 + Ga0059261_2191 0.84 -1.2 2,299,779 + Ga0059261_2191 0.84 -2.4 2,299,779 + Ga0059261_2191 0.84 -5.4 2,299,780 - Ga0059261_2191 0.84 -3.4 2,299,870 + -3.7 2,299,870 + -4.1 2,299,870 + -3.2 2,299,870 + -2.7 2,299,870 + -4.3 2,299,870 + -2.3 2,299,871 - -2.5 2,299,871 - -2.7 2,299,871 - -2.9 2,299,886 + -2.9 2,299,886 + -2.9 2,299,886 + -2.6 2,299,886 + -1.4 2,299,886 + -0.3 2,299,887 - -0.1 2,299,887 - -0.7 2,299,899 + -1.7 2,299,899 + -3.3 2,299,899 + -2.8 2,299,899 + -1.3 2,299,900 - +0.1 2,299,900 - -0.5 2,299,900 - -0.3 2,301,394 - Ga0059261_2194 0.21 -2.0 2,301,394 - Ga0059261_2194 0.21 -3.6 2,301,394 - Ga0059261_2194 0.21 +0.5 2,301,394 - Ga0059261_2194 0.21 -3.7 2,301,394 - Ga0059261_2194 0.21 -3.7 2,301,406 - Ga0059261_2194 0.22 -4.2 2,301,406 - Ga0059261_2194 0.22 -3.5 2,301,406 - Ga0059261_2194 0.22 -2.4 2,301,406 - Ga0059261_2194 0.22 -2.8 2,301,709 - Ga0059261_2194 0.60 -3.3 2,301,709 - Ga0059261_2194 0.60 -3.7 2,301,727 + Ga0059261_2194 0.62 -1.6 2,301,933 + Ga0059261_2194 0.87 -2.1 2,301,933 + Ga0059261_2194 0.87 -3.2 2,302,024 - -3.7 2,302,024 - -2.9 2,302,056 + -0.3 2,302,056 + -0.2 2,302,056 + -0.2 2,302,056 + -0.1 2,302,056 + -1.1 2,302,056 + +1.5 2,302,056 + -0.2 2,302,057 - -0.1 2,302,057 - +0.9 2,302,057 - -0.1 2,302,057 - -0.1 2,302,065 - +0.4 2,302,065 - +0.3 2,302,065 - +0.1 2,302,065 - +0.5 2,302,103 + Ga0059261_2195 0.12 -2.9 2,302,103 + Ga0059261_2195 0.12 -2.2 2,302,103 + Ga0059261_2195 0.12 -3.3 2,302,103 + Ga0059261_2195 0.12 -0.7 2,302,104 - Ga0059261_2195 0.12 +0.6 2,302,104 - Ga0059261_2195 0.12 +0.7 2,302,202 + Ga0059261_2195 0.29 +0.3 2,302,202 + Ga0059261_2195 0.29 -0.9 2,302,203 - Ga0059261_2195 0.29 -0.2 2,302,203 - Ga0059261_2195 0.29 +0.3 2,302,203 - Ga0059261_2195 0.29 +0.4 2,302,203 - Ga0059261_2195 0.29 -0.5
Or see this region's nucleotide sequence