Experiment: L-Glutamic (C) and 0.25mM L-Methionine; no added vitamins
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Ga0059261_0289 and Ga0059261_0290 are separated by 63 nucleotides Ga0059261_0290 and Ga0059261_0291 are separated by 64 nucleotides Ga0059261_0291 and Ga0059261_0292 are separated by 6 nucleotides
Ga0059261_0289: Ga0059261_0289 - thiamine biosynthesis protein ThiS, at 274,143 to 275,153
_0289
Ga0059261_0290: Ga0059261_0290 - 3-dehydroquinate dehydratase (EC 4.2.1.10), at 275,217 to 275,651
_0290
Ga0059261_0291: Ga0059261_0291 - acetyl-CoA carboxylase, biotin carboxyl carrier protein, at 275,716 to 276,201
_0291
Ga0059261_0292: Ga0059261_0292 - acetyl-CoA carboxylase, biotin carboxylase subunit, at 276,208 to 277,572
_0292
Position (kb) 275
276 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 275.179 kb on - strand at 275.179 kb on - strand at 275.180 kb on + strand at 275.180 kb on + strand at 275.180 kb on + strand at 275.180 kb on + strand at 275.181 kb on - strand at 275.181 kb on - strand at 275.181 kb on - strand at 275.181 kb on - strand at 275.181 kb on - strand at 275.181 kb on - strand at 275.181 kb on - strand at 275.226 kb on - strand at 275.232 kb on + strand at 275.232 kb on + strand at 275.232 kb on + strand at 275.232 kb on + strand at 275.232 kb on + strand at 275.232 kb on + strand at 275.233 kb on - strand at 275.233 kb on - strand at 275.233 kb on - strand at 275.233 kb on - strand at 275.233 kb on - strand at 275.233 kb on - strand at 275.236 kb on + strand at 275.287 kb on - strand, within Ga0059261_0290 at 275.454 kb on + strand, within Ga0059261_0290 at 275.454 kb on + strand, within Ga0059261_0290 at 275.455 kb on - strand at 275.455 kb on - strand, within Ga0059261_0290 at 275.455 kb on - strand, within Ga0059261_0290 at 275.479 kb on - strand, within Ga0059261_0290 at 275.479 kb on - strand, within Ga0059261_0290 at 275.486 kb on + strand, within Ga0059261_0290 at 275.523 kb on - strand, within Ga0059261_0290 at 275.565 kb on + strand, within Ga0059261_0290 at 275.565 kb on + strand, within Ga0059261_0290 at 275.565 kb on + strand, within Ga0059261_0290 at 275.565 kb on + strand, within Ga0059261_0290 at 275.565 kb on + strand, within Ga0059261_0290 at 275.565 kb on + strand, within Ga0059261_0290 at 275.565 kb on + strand, within Ga0059261_0290 at 275.566 kb on - strand, within Ga0059261_0290 at 275.566 kb on - strand, within Ga0059261_0290 at 275.566 kb on - strand, within Ga0059261_0290 at 275.616 kb on + strand at 275.616 kb on + strand at 275.616 kb on + strand at 275.616 kb on + strand at 275.616 kb on + strand at 275.616 kb on + strand at 275.665 kb on + strand at 275.665 kb on + strand at 275.667 kb on + strand at 275.667 kb on + strand at 275.667 kb on + strand at 275.667 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamic (C) and 0.25mM L-Methionine; no added vitamins remove 275,179 - -0.8 275,179 - +0.2 275,180 + +2.1 275,180 + -0.3 275,180 + -1.5 275,180 + +0.8 275,181 - -0.9 275,181 - -0.2 275,181 - -1.5 275,181 - -2.5 275,181 - -2.0 275,181 - -2.3 275,181 - -0.2 275,226 - -3.1 275,232 + -3.5 275,232 + -4.4 275,232 + -3.9 275,232 + -5.9 275,232 + -5.8 275,232 + -3.2 275,233 - -2.7 275,233 - -3.7 275,233 - -4.0 275,233 - -3.2 275,233 - -3.5 275,233 - -4.0 275,236 + -2.9 275,287 - Ga0059261_0290 0.16 -4.0 275,454 + Ga0059261_0290 0.54 -2.5 275,454 + Ga0059261_0290 0.54 -2.2 275,455 - -1.3 275,455 - Ga0059261_0290 0.55 -3.1 275,455 - Ga0059261_0290 0.55 -4.0 275,479 - Ga0059261_0290 0.60 -3.5 275,479 - Ga0059261_0290 0.60 -3.0 275,486 + Ga0059261_0290 0.62 -4.7 275,523 - Ga0059261_0290 0.70 -3.2 275,565 + Ga0059261_0290 0.80 -4.7 275,565 + Ga0059261_0290 0.80 -4.0 275,565 + Ga0059261_0290 0.80 -2.9 275,565 + Ga0059261_0290 0.80 -4.6 275,565 + Ga0059261_0290 0.80 -3.9 275,565 + Ga0059261_0290 0.80 -3.5 275,565 + Ga0059261_0290 0.80 -3.6 275,566 - Ga0059261_0290 0.80 -4.4 275,566 - Ga0059261_0290 0.80 -3.5 275,566 - Ga0059261_0290 0.80 -3.0 275,616 + -2.7 275,616 + -5.2 275,616 + -1.4 275,616 + -4.8 275,616 + -1.6 275,616 + -5.4 275,665 + +0.5 275,665 + -0.0 275,667 + +0.7 275,667 + +0.1 275,667 + -1.5 275,667 + -0.2
Or see this region's nucleotide sequence