Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS03000

Experiment: D-Serine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02990 and BWI76_RS02995 are separated by 12 nucleotidesBWI76_RS02995 and BWI76_RS03000 are separated by 36 nucleotidesBWI76_RS03000 and BWI76_RS03005 are separated by 192 nucleotidesBWI76_RS03005 and BWI76_RS03010 are separated by 190 nucleotides BWI76_RS02990: BWI76_RS02990 - aspartate carbamoyltransferase regulatory subunit, at 613,512 to 613,973 _RS02990 BWI76_RS02995: BWI76_RS02995 - aspartate carbamoyltransferase, at 613,986 to 614,921 _RS02995 BWI76_RS03000: BWI76_RS03000 - PyrBI operon leader peptide, at 614,958 to 615,062 _RS03000 BWI76_RS03005: BWI76_RS03005 - hypothetical protein, at 615,255 to 615,707 _RS03005 BWI76_RS03010: BWI76_RS03010 - ornithine carbamoyltransferase, at 615,898 to 616,902 _RS03010 Position (kb) 614 615 616Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 614.067 kb on + strandat 614.248 kb on - strand, within BWI76_RS02995at 614.303 kb on - strand, within BWI76_RS02995at 614.318 kb on + strand, within BWI76_RS02995at 614.407 kb on - strand, within BWI76_RS02995at 614.469 kb on + strand, within BWI76_RS02995at 614.469 kb on + strand, within BWI76_RS02995at 614.477 kb on - strand, within BWI76_RS02995at 614.488 kb on - strand, within BWI76_RS02995at 614.503 kb on - strand, within BWI76_RS02995at 614.557 kb on - strand, within BWI76_RS02995at 614.919 kb on - strandat 615.106 kb on - strandat 615.140 kb on - strandat 615.140 kb on - strandat 615.279 kb on + strandat 615.300 kb on + strandat 615.323 kb on + strand, within BWI76_RS03005at 615.331 kb on - strand, within BWI76_RS03005at 615.331 kb on - strand, within BWI76_RS03005at 615.356 kb on + strand, within BWI76_RS03005at 615.617 kb on + strand, within BWI76_RS03005at 615.682 kb on - strandat 615.739 kb on - strandat 615.800 kb on - strandat 615.823 kb on + strandat 615.829 kb on - strandat 615.858 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Serine (N)
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614,067 + -4.2
614,248 - BWI76_RS02995 0.28 -5.7
614,303 - BWI76_RS02995 0.34 -2.7
614,318 + BWI76_RS02995 0.35 -4.8
614,407 - BWI76_RS02995 0.45 -5.8
614,469 + BWI76_RS02995 0.52 -3.5
614,469 + BWI76_RS02995 0.52 -0.8
614,477 - BWI76_RS02995 0.52 -5.3
614,488 - BWI76_RS02995 0.54 -4.0
614,503 - BWI76_RS02995 0.55 -0.3
614,557 - BWI76_RS02995 0.61 -2.0
614,919 - -4.1
615,106 - -1.2
615,140 - -0.9
615,140 - +0.6
615,279 + +0.7
615,300 + +0.3
615,323 + BWI76_RS03005 0.15 -0.2
615,331 - BWI76_RS03005 0.17 -0.2
615,331 - BWI76_RS03005 0.17 -0.3
615,356 + BWI76_RS03005 0.22 +0.6
615,617 + BWI76_RS03005 0.80 -0.6
615,682 - +1.6
615,739 - +0.2
615,800 - -0.7
615,823 + -0.0
615,829 - +1.0
615,858 + +0.1

Or see this region's nucleotide sequence