Experiment: antimonate 0.005 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_586 and Pf6N2E2_587 are separated by 147 nucleotides Pf6N2E2_587 and Pf6N2E2_588 are separated by 65 nucleotides Pf6N2E2_588 and Pf6N2E2_589 are separated by 89 nucleotides
Pf6N2E2_586 - Membrane-bound lytic murein transglycosylase D precursor (EC 3.2.1.-), at 663,474 to 664,874
_586
Pf6N2E2_587 - Hydroxyacylglutathione hydrolase (EC 3.1.2.6), at 665,022 to 665,789
_587
Pf6N2E2_588 - FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-), at 665,855 to 666,613
_588
Pf6N2E2_589 - Ribonuclease HI (EC 3.1.26.4), at 666,703 to 667,155
_589
Position (kb)
665
666 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 664.139 kb on - strand, within Pf6N2E2_586 at 664.189 kb on + strand, within Pf6N2E2_586 at 664.189 kb on + strand, within Pf6N2E2_586 at 664.189 kb on + strand, within Pf6N2E2_586 at 664.190 kb on - strand, within Pf6N2E2_586 at 664.190 kb on - strand, within Pf6N2E2_586 at 664.190 kb on - strand, within Pf6N2E2_586 at 664.190 kb on - strand, within Pf6N2E2_586 at 664.190 kb on - strand, within Pf6N2E2_586 at 664.190 kb on - strand, within Pf6N2E2_586 at 664.190 kb on - strand, within Pf6N2E2_586 at 664.295 kb on - strand, within Pf6N2E2_586 at 664.352 kb on - strand, within Pf6N2E2_586 at 664.399 kb on + strand at 664.399 kb on + strand at 664.400 kb on - strand, within Pf6N2E2_586 at 664.400 kb on - strand, within Pf6N2E2_586 at 664.427 kb on - strand, within Pf6N2E2_586 at 664.480 kb on + strand, within Pf6N2E2_586 at 664.495 kb on + strand, within Pf6N2E2_586 at 664.567 kb on + strand, within Pf6N2E2_586 at 664.568 kb on - strand, within Pf6N2E2_586 at 664.568 kb on - strand, within Pf6N2E2_586 at 664.568 kb on - strand, within Pf6N2E2_586 at 664.568 kb on - strand, within Pf6N2E2_586 at 664.576 kb on + strand, within Pf6N2E2_586 at 664.576 kb on + strand, within Pf6N2E2_586 at 664.933 kb on + strand at 665.004 kb on + strand at 665.023 kb on + strand at 665.023 kb on + strand at 665.024 kb on - strand at 665.030 kb on + strand at 665.030 kb on + strand at 665.031 kb on - strand at 665.031 kb on - strand at 665.282 kb on + strand, within Pf6N2E2_587 at 665.282 kb on + strand, within Pf6N2E2_587 at 665.282 kb on + strand, within Pf6N2E2_587 at 665.282 kb on + strand, within Pf6N2E2_587 at 665.283 kb on - strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.284 kb on + strand, within Pf6N2E2_587 at 665.285 kb on - strand, within Pf6N2E2_587 at 665.285 kb on - strand, within Pf6N2E2_587 at 665.299 kb on + strand, within Pf6N2E2_587 at 665.299 kb on + strand, within Pf6N2E2_587 at 665.300 kb on - strand, within Pf6N2E2_587 at 665.310 kb on - strand, within Pf6N2E2_587 at 665.431 kb on + strand, within Pf6N2E2_587 at 665.431 kb on + strand, within Pf6N2E2_587 at 665.431 kb on + strand, within Pf6N2E2_587 at 665.434 kb on + strand, within Pf6N2E2_587 at 665.470 kb on + strand, within Pf6N2E2_587 at 665.470 kb on + strand, within Pf6N2E2_587 at 665.736 kb on + strand at 665.749 kb on + strand at 665.749 kb on + strand at 665.749 kb on + strand at 665.749 kb on + strand at 665.749 kb on + strand at 665.749 kb on + strand at 665.750 kb on - strand at 665.750 kb on - strand at 665.750 kb on - strand at 665.750 kb on - strand at 665.785 kb on - strand at 665.785 kb on - strand at 665.785 kb on - strand at 665.826 kb on + strand at 665.826 kb on + strand at 665.827 kb on - strand at 666.018 kb on - strand, within Pf6N2E2_588 at 666.489 kb on - strand, within Pf6N2E2_588 at 666.611 kb on + strand at 666.694 kb on + strand at 666.694 kb on + strand at 666.716 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction antimonate 0.005 M remove 664,139 - Pf6N2E2_586 0.47 -0.7 664,189 + Pf6N2E2_586 0.51 +0.6 664,189 + Pf6N2E2_586 0.51 -0.4 664,189 + Pf6N2E2_586 0.51 -0.8 664,190 - Pf6N2E2_586 0.51 +1.1 664,190 - Pf6N2E2_586 0.51 -0.0 664,190 - Pf6N2E2_586 0.51 +1.3 664,190 - Pf6N2E2_586 0.51 -0.0 664,190 - Pf6N2E2_586 0.51 +0.2 664,190 - Pf6N2E2_586 0.51 -1.0 664,190 - Pf6N2E2_586 0.51 -0.1 664,295 - Pf6N2E2_586 0.59 -1.4 664,352 - Pf6N2E2_586 0.63 +1.2 664,399 + +1.6 664,399 + -0.8 664,400 - Pf6N2E2_586 0.66 -0.6 664,400 - Pf6N2E2_586 0.66 -0.8 664,427 - Pf6N2E2_586 0.68 +0.7 664,480 + Pf6N2E2_586 0.72 +0.2 664,495 + Pf6N2E2_586 0.73 -1.1 664,567 + Pf6N2E2_586 0.78 +0.6 664,568 - Pf6N2E2_586 0.78 -0.6 664,568 - Pf6N2E2_586 0.78 +0.9 664,568 - Pf6N2E2_586 0.78 -0.9 664,568 - Pf6N2E2_586 0.78 +0.9 664,576 + Pf6N2E2_586 0.79 -0.8 664,576 + Pf6N2E2_586 0.79 -0.7 664,933 + -2.1 665,004 + -1.0 665,023 + -1.8 665,023 + -1.6 665,024 - +0.6 665,030 + +0.9 665,030 + -0.4 665,031 - +0.0 665,031 - -1.2 665,282 + Pf6N2E2_587 0.34 -2.8 665,282 + Pf6N2E2_587 0.34 -1.8 665,282 + Pf6N2E2_587 0.34 +0.7 665,282 + Pf6N2E2_587 0.34 -0.0 665,283 - Pf6N2E2_587 0.34 +0.1 665,284 + Pf6N2E2_587 0.34 -2.0 665,284 + Pf6N2E2_587 0.34 -0.8 665,284 + Pf6N2E2_587 0.34 -1.2 665,284 + Pf6N2E2_587 0.34 -1.0 665,284 + Pf6N2E2_587 0.34 -1.6 665,284 + Pf6N2E2_587 0.34 +1.6 665,284 + Pf6N2E2_587 0.34 -1.4 665,284 + Pf6N2E2_587 0.34 -1.1 665,285 - Pf6N2E2_587 0.34 -0.0 665,285 - Pf6N2E2_587 0.34 -1.5 665,299 + Pf6N2E2_587 0.36 -2.7 665,299 + Pf6N2E2_587 0.36 -1.3 665,300 - Pf6N2E2_587 0.36 -1.0 665,310 - Pf6N2E2_587 0.38 +0.8 665,431 + Pf6N2E2_587 0.53 -0.1 665,431 + Pf6N2E2_587 0.53 -0.7 665,431 + Pf6N2E2_587 0.53 -3.0 665,434 + Pf6N2E2_587 0.54 -0.9 665,470 + Pf6N2E2_587 0.58 -2.4 665,470 + Pf6N2E2_587 0.58 -0.8 665,736 + -0.9 665,749 + -1.2 665,749 + -0.9 665,749 + -1.4 665,749 + -0.6 665,749 + -3.3 665,749 + -0.8 665,750 - +1.6 665,750 - +0.0 665,750 - +0.5 665,750 - -0.2 665,785 - -0.5 665,785 - +0.8 665,785 - +0.8 665,826 + -2.5 665,826 + -1.5 665,827 - +1.0 666,018 - Pf6N2E2_588 0.21 +0.7 666,489 - Pf6N2E2_588 0.84 +1.1 666,611 + -0.0 666,694 + -1.0 666,694 + +1.2 666,716 + -1.0
Or see this region's nucleotide sequence