Experiment: antimonate 0.005 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2828 and Pf6N2E2_2829 are separated by 206 nucleotides Pf6N2E2_2829 and Pf6N2E2_2830 are separated by 10 nucleotides Pf6N2E2_2830 and Pf6N2E2_2831 are separated by 12 nucleotides
Pf6N2E2_2828 - PhaK-like protein, at 3,317,105 to 3,318,358
_2828
Pf6N2E2_2829 - 4-carboxymuconolactone decarboxylase (EC 4.1.1.44), at 3,318,565 to 3,318,957
_2829
Pf6N2E2_2830 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 3,318,968 to 3,319,768
_2830
Pf6N2E2_2831 - 3-carboxy-cis,cis-muconate cycloisomerase (EC 5.5.1.2), at 3,319,781 to 3,321,145
_2831
Position (kb)
3318
3319 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3317.621 kb on + strand, within Pf6N2E2_2828 at 3317.621 kb on + strand, within Pf6N2E2_2828 at 3317.622 kb on - strand, within Pf6N2E2_2828 at 3317.622 kb on - strand, within Pf6N2E2_2828 at 3317.622 kb on - strand, within Pf6N2E2_2828 at 3317.736 kb on - strand, within Pf6N2E2_2828 at 3317.736 kb on - strand, within Pf6N2E2_2828 at 3318.050 kb on + strand, within Pf6N2E2_2828 at 3318.050 kb on + strand, within Pf6N2E2_2828 at 3318.050 kb on + strand, within Pf6N2E2_2828 at 3318.050 kb on + strand, within Pf6N2E2_2828 at 3318.050 kb on + strand, within Pf6N2E2_2828 at 3318.050 kb on + strand, within Pf6N2E2_2828 at 3318.062 kb on + strand, within Pf6N2E2_2828 at 3318.093 kb on + strand, within Pf6N2E2_2828 at 3318.093 kb on + strand, within Pf6N2E2_2828 at 3318.094 kb on - strand, within Pf6N2E2_2828 at 3318.094 kb on - strand, within Pf6N2E2_2828 at 3318.153 kb on - strand, within Pf6N2E2_2828 at 3318.227 kb on + strand, within Pf6N2E2_2828 at 3318.228 kb on - strand, within Pf6N2E2_2828 at 3318.296 kb on + strand at 3318.296 kb on + strand at 3318.297 kb on - strand at 3318.301 kb on + strand at 3318.301 kb on + strand at 3318.302 kb on - strand at 3318.302 kb on - strand at 3318.302 kb on - strand at 3318.356 kb on + strand at 3318.356 kb on + strand at 3318.356 kb on + strand at 3318.357 kb on - strand at 3318.357 kb on - strand at 3318.383 kb on - strand at 3318.405 kb on + strand at 3318.405 kb on + strand at 3318.463 kb on + strand at 3318.463 kb on + strand at 3318.463 kb on + strand at 3318.463 kb on + strand at 3318.464 kb on - strand at 3318.647 kb on + strand, within Pf6N2E2_2829 at 3318.647 kb on + strand, within Pf6N2E2_2829 at 3318.647 kb on + strand, within Pf6N2E2_2829 at 3318.648 kb on - strand, within Pf6N2E2_2829 at 3318.648 kb on - strand, within Pf6N2E2_2829 at 3318.648 kb on - strand, within Pf6N2E2_2829 at 3318.768 kb on + strand, within Pf6N2E2_2829 at 3318.769 kb on - strand, within Pf6N2E2_2829 at 3318.769 kb on - strand, within Pf6N2E2_2829 at 3318.919 kb on + strand at 3318.920 kb on - strand at 3318.938 kb on + strand at 3318.938 kb on + strand at 3319.054 kb on - strand, within Pf6N2E2_2830 at 3319.065 kb on + strand, within Pf6N2E2_2830 at 3319.331 kb on + strand, within Pf6N2E2_2830 at 3319.359 kb on - strand, within Pf6N2E2_2830 at 3319.431 kb on + strand, within Pf6N2E2_2830 at 3319.431 kb on + strand, within Pf6N2E2_2830 at 3319.431 kb on + strand, within Pf6N2E2_2830 at 3319.507 kb on + strand, within Pf6N2E2_2830 at 3319.507 kb on + strand, within Pf6N2E2_2830 at 3319.731 kb on + strand at 3319.731 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction antimonate 0.005 M remove 3,317,621 + Pf6N2E2_2828 0.41 -0.2 3,317,621 + Pf6N2E2_2828 0.41 -2.0 3,317,622 - Pf6N2E2_2828 0.41 -0.3 3,317,622 - Pf6N2E2_2828 0.41 +0.2 3,317,622 - Pf6N2E2_2828 0.41 +0.5 3,317,736 - Pf6N2E2_2828 0.50 +0.1 3,317,736 - Pf6N2E2_2828 0.50 -0.2 3,318,050 + Pf6N2E2_2828 0.75 +0.2 3,318,050 + Pf6N2E2_2828 0.75 -0.1 3,318,050 + Pf6N2E2_2828 0.75 +0.2 3,318,050 + Pf6N2E2_2828 0.75 -0.2 3,318,050 + Pf6N2E2_2828 0.75 +0.1 3,318,050 + Pf6N2E2_2828 0.75 -0.0 3,318,062 + Pf6N2E2_2828 0.76 -0.1 3,318,093 + Pf6N2E2_2828 0.79 -0.7 3,318,093 + Pf6N2E2_2828 0.79 -1.1 3,318,094 - Pf6N2E2_2828 0.79 -0.5 3,318,094 - Pf6N2E2_2828 0.79 +0.5 3,318,153 - Pf6N2E2_2828 0.84 +0.3 3,318,227 + Pf6N2E2_2828 0.89 -0.6 3,318,228 - Pf6N2E2_2828 0.90 +0.6 3,318,296 + +0.6 3,318,296 + -0.3 3,318,297 - +0.7 3,318,301 + -0.4 3,318,301 + -0.4 3,318,302 - -0.5 3,318,302 - +2.4 3,318,302 - +0.4 3,318,356 + -1.3 3,318,356 + +0.5 3,318,356 + -0.1 3,318,357 - +0.9 3,318,357 - +0.6 3,318,383 - -0.0 3,318,405 + -0.5 3,318,405 + +1.9 3,318,463 + +0.2 3,318,463 + -1.2 3,318,463 + -1.2 3,318,463 + +0.4 3,318,464 - +1.0 3,318,647 + Pf6N2E2_2829 0.21 -0.7 3,318,647 + Pf6N2E2_2829 0.21 -2.3 3,318,647 + Pf6N2E2_2829 0.21 -0.8 3,318,648 - Pf6N2E2_2829 0.21 +0.6 3,318,648 - Pf6N2E2_2829 0.21 -1.4 3,318,648 - Pf6N2E2_2829 0.21 -1.6 3,318,768 + Pf6N2E2_2829 0.52 -1.5 3,318,769 - Pf6N2E2_2829 0.52 +0.1 3,318,769 - Pf6N2E2_2829 0.52 +0.6 3,318,919 + +0.4 3,318,920 - +0.4 3,318,938 + -2.5 3,318,938 + +0.1 3,319,054 - Pf6N2E2_2830 0.11 +0.8 3,319,065 + Pf6N2E2_2830 0.12 +2.5 3,319,331 + Pf6N2E2_2830 0.45 +0.4 3,319,359 - Pf6N2E2_2830 0.49 -1.1 3,319,431 + Pf6N2E2_2830 0.58 +0.9 3,319,431 + Pf6N2E2_2830 0.58 +1.3 3,319,431 + Pf6N2E2_2830 0.58 -0.5 3,319,507 + Pf6N2E2_2830 0.67 -0.8 3,319,507 + Pf6N2E2_2830 0.67 -0.3 3,319,731 + +0.1 3,319,731 + -0.4
Or see this region's nucleotide sequence