Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20215

Experiment: L-Tryptophan (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20205 and BWI76_RS20210 are separated by 87 nucleotidesBWI76_RS20210 and BWI76_RS20215 are separated by 53 nucleotidesBWI76_RS20215 and BWI76_RS20220 are separated by 76 nucleotidesBWI76_RS20220 and BWI76_RS20225 are separated by 11 nucleotides BWI76_RS20205: BWI76_RS20205 - aminotransferase AlaT, at 4,234,466 to 4,235,683 _RS20205 BWI76_RS20210: BWI76_RS20210 - 5'-deoxynucleotidase, at 4,235,771 to 4,236,370 _RS20210 BWI76_RS20215: BWI76_RS20215 - transcriptional regulator, at 4,236,424 to 4,238,256 _RS20215 BWI76_RS20220: BWI76_RS20220 - sugar phosphatase, at 4,238,333 to 4,238,992 _RS20220 BWI76_RS20225: BWI76_RS20225 - hypothetical protein, at 4,239,004 to 4,239,498 _RS20225 Position (kb) 4236 4237 4238 4239Strain fitness (log2 ratio) -2 -1 0 1 2at 4235.442 kb on - strand, within BWI76_RS20205at 4235.459 kb on + strand, within BWI76_RS20205at 4235.459 kb on + strand, within BWI76_RS20205at 4235.757 kb on + strandat 4235.757 kb on + strandat 4235.872 kb on - strand, within BWI76_RS20210at 4235.935 kb on + strand, within BWI76_RS20210at 4235.943 kb on - strand, within BWI76_RS20210at 4235.943 kb on - strand, within BWI76_RS20210at 4235.943 kb on - strand, within BWI76_RS20210at 4236.053 kb on - strand, within BWI76_RS20210at 4236.285 kb on + strand, within BWI76_RS20210at 4236.290 kb on + strand, within BWI76_RS20210at 4236.390 kb on + strandat 4236.458 kb on + strandat 4236.619 kb on - strand, within BWI76_RS20215at 4236.684 kb on - strand, within BWI76_RS20215at 4236.859 kb on + strand, within BWI76_RS20215at 4237.010 kb on - strand, within BWI76_RS20215at 4237.167 kb on - strand, within BWI76_RS20215at 4237.265 kb on + strand, within BWI76_RS20215at 4237.357 kb on - strand, within BWI76_RS20215at 4237.658 kb on + strand, within BWI76_RS20215at 4237.666 kb on - strand, within BWI76_RS20215at 4237.921 kb on + strand, within BWI76_RS20215at 4237.930 kb on + strand, within BWI76_RS20215at 4237.967 kb on - strand, within BWI76_RS20215at 4238.082 kb on + strandat 4238.124 kb on + strandat 4238.156 kb on + strandat 4238.231 kb on - strandat 4238.251 kb on + strandat 4238.558 kb on - strand, within BWI76_RS20220at 4238.720 kb on - strand, within BWI76_RS20220at 4238.738 kb on - strand, within BWI76_RS20220at 4238.979 kb on + strandat 4238.979 kb on + strandat 4238.979 kb on + strandat 4238.979 kb on + strandat 4238.987 kb on - strandat 4239.045 kb on - strandat 4239.045 kb on - strandat 4239.226 kb on + strand, within BWI76_RS20225

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Tryptophan (N)
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4,235,442 - BWI76_RS20205 0.80 -0.7
4,235,459 + BWI76_RS20205 0.82 -0.7
4,235,459 + BWI76_RS20205 0.82 -1.2
4,235,757 + +1.3
4,235,757 + +0.1
4,235,872 - BWI76_RS20210 0.17 +0.8
4,235,935 + BWI76_RS20210 0.27 -0.6
4,235,943 - BWI76_RS20210 0.29 -0.2
4,235,943 - BWI76_RS20210 0.29 -0.4
4,235,943 - BWI76_RS20210 0.29 -1.6
4,236,053 - BWI76_RS20210 0.47 -1.7
4,236,285 + BWI76_RS20210 0.86 -0.5
4,236,290 + BWI76_RS20210 0.86 -0.5
4,236,390 + -0.3
4,236,458 + +1.4
4,236,619 - BWI76_RS20215 0.11 +0.4
4,236,684 - BWI76_RS20215 0.14 +1.4
4,236,859 + BWI76_RS20215 0.24 +0.4
4,237,010 - BWI76_RS20215 0.32 +0.4
4,237,167 - BWI76_RS20215 0.41 -0.0
4,237,265 + BWI76_RS20215 0.46 +1.4
4,237,357 - BWI76_RS20215 0.51 -0.9
4,237,658 + BWI76_RS20215 0.67 -0.9
4,237,666 - BWI76_RS20215 0.68 -0.8
4,237,921 + BWI76_RS20215 0.82 +0.4
4,237,930 + BWI76_RS20215 0.82 +0.1
4,237,967 - BWI76_RS20215 0.84 +0.5
4,238,082 + +0.4
4,238,124 + -0.0
4,238,156 + -0.7
4,238,231 - -0.0
4,238,251 + -2.0
4,238,558 - BWI76_RS20220 0.34 +0.1
4,238,720 - BWI76_RS20220 0.59 -0.1
4,238,738 - BWI76_RS20220 0.61 -0.0
4,238,979 + -0.7
4,238,979 + -0.5
4,238,979 + +0.2
4,238,979 + +2.1
4,238,987 - -0.4
4,239,045 - +0.4
4,239,045 - +0.1
4,239,226 + BWI76_RS20225 0.45 +0.4

Or see this region's nucleotide sequence