Experiment: L-Tryptophan (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16930 and BWI76_RS16935 are separated by 63 nucleotides BWI76_RS16935 and BWI76_RS16940 are separated by 31 nucleotides BWI76_RS16940 and BWI76_RS16945 are separated by 79 nucleotides
BWI76_RS16930: BWI76_RS16930 - transcriptional regulator, at 3,511,058 to 3,511,864
_RS16930
BWI76_RS16935: BWI76_RS16935 - shikimate dehydrogenase, at 3,511,928 to 3,512,764
_RS16935
BWI76_RS16940: BWI76_RS16940 - MFS transporter, at 3,512,796 to 3,514,115
_RS16940
BWI76_RS16945: BWI76_RS16945 - 4-hydroxyphenylpyruvate dioxygenase, at 3,514,195 to 3,516,048
_RS16945
Position (kb)
3512
3513
3514
3515 Strain fitness (log2 ratio)
-1
0
1 at 3511.957 kb on + strand at 3512.130 kb on - strand, within BWI76_RS16935 at 3512.708 kb on - strand at 3513.503 kb on + strand, within BWI76_RS16940 at 3513.684 kb on - strand, within BWI76_RS16940 at 3513.762 kb on - strand, within BWI76_RS16940 at 3513.906 kb on + strand, within BWI76_RS16940 at 3513.956 kb on - strand, within BWI76_RS16940 at 3513.981 kb on + strand, within BWI76_RS16940 at 3514.156 kb on - strand at 3514.204 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Tryptophan (N) remove 3,511,957 + -0.3 3,512,130 - BWI76_RS16935 0.24 +0.6 3,512,708 - -0.3 3,513,503 + BWI76_RS16940 0.54 -0.0 3,513,684 - BWI76_RS16940 0.67 -0.1 3,513,762 - BWI76_RS16940 0.73 +0.0 3,513,906 + BWI76_RS16940 0.84 +0.5 3,513,956 - BWI76_RS16940 0.88 -0.3 3,513,981 + BWI76_RS16940 0.90 -0.4 3,514,156 - +0.6 3,514,204 + +0.5
Or see this region's nucleotide sequence