Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02000

Experiment: L-Tryptophan (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01990 and BWI76_RS01995 overlap by 16 nucleotidesBWI76_RS01995 and BWI76_RS02000 are separated by 84 nucleotidesBWI76_RS02000 and BWI76_RS02005 are separated by 258 nucleotidesBWI76_RS02005 and BWI76_RS02010 are separated by 36 nucleotides BWI76_RS01990: BWI76_RS01990 - cyclic diguanylate phosphodiesterase, at 409,135 to 410,739 _RS01990 BWI76_RS01995: BWI76_RS01995 - AraC family transcriptional regulator, at 410,724 to 411,053 _RS01995 BWI76_RS02000: BWI76_RS02000 - transcriptional regulator, at 411,138 to 411,596 _RS02000 BWI76_RS02005: BWI76_RS02005 - glutathione S-transferase, at 411,855 to 412,523 _RS02005 BWI76_RS02010: BWI76_RS02010 - hypothetical protein, at 412,560 to 412,760 _RS02010 Position (kb) 411 412Strain fitness (log2 ratio) -2 -1 0 1 2at 410.141 kb on - strand, within BWI76_RS01990at 410.142 kb on - strand, within BWI76_RS01990at 410.279 kb on - strand, within BWI76_RS01990at 410.291 kb on + strand, within BWI76_RS01990at 410.413 kb on + strand, within BWI76_RS01990at 410.422 kb on + strand, within BWI76_RS01990at 410.430 kb on - strand, within BWI76_RS01990at 410.625 kb on - strandat 410.915 kb on - strand, within BWI76_RS01995at 411.091 kb on - strandat 411.314 kb on + strand, within BWI76_RS02000at 411.385 kb on + strand, within BWI76_RS02000at 411.393 kb on - strand, within BWI76_RS02000at 411.393 kb on - strand, within BWI76_RS02000at 411.454 kb on + strand, within BWI76_RS02000at 411.454 kb on + strand, within BWI76_RS02000at 411.454 kb on + strand, within BWI76_RS02000at 411.454 kb on + strand, within BWI76_RS02000at 411.454 kb on + strand, within BWI76_RS02000at 411.462 kb on - strand, within BWI76_RS02000at 411.462 kb on - strand, within BWI76_RS02000at 411.462 kb on - strand, within BWI76_RS02000at 411.462 kb on - strand, within BWI76_RS02000at 411.699 kb on + strandat 411.699 kb on + strandat 411.699 kb on + strandat 411.699 kb on + strandat 411.702 kb on - strandat 411.707 kb on - strandat 411.707 kb on - strandat 411.707 kb on - strandat 411.707 kb on - strandat 411.727 kb on + strandat 411.864 kb on - strandat 411.943 kb on - strand, within BWI76_RS02005at 411.951 kb on - strand, within BWI76_RS02005at 411.953 kb on + strand, within BWI76_RS02005at 411.959 kb on + strand, within BWI76_RS02005at 412.056 kb on + strand, within BWI76_RS02005at 412.078 kb on + strand, within BWI76_RS02005at 412.085 kb on - strand, within BWI76_RS02005at 412.145 kb on + strand, within BWI76_RS02005at 412.150 kb on + strand, within BWI76_RS02005at 412.213 kb on - strand, within BWI76_RS02005at 412.253 kb on + strand, within BWI76_RS02005at 412.261 kb on - strand, within BWI76_RS02005at 412.285 kb on - strand, within BWI76_RS02005at 412.322 kb on + strand, within BWI76_RS02005at 412.461 kb on - strandat 412.463 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Tryptophan (N)
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410,141 - BWI76_RS01990 0.63 -1.6
410,142 - BWI76_RS01990 0.63 -2.4
410,279 - BWI76_RS01990 0.71 -0.1
410,291 + BWI76_RS01990 0.72 -1.0
410,413 + BWI76_RS01990 0.80 -0.4
410,422 + BWI76_RS01990 0.80 -2.2
410,430 - BWI76_RS01990 0.81 -0.8
410,625 - -0.9
410,915 - BWI76_RS01995 0.58 -1.3
411,091 - +0.8
411,314 + BWI76_RS02000 0.38 -0.4
411,385 + BWI76_RS02000 0.54 +0.6
411,393 - BWI76_RS02000 0.56 +0.2
411,393 - BWI76_RS02000 0.56 -0.2
411,454 + BWI76_RS02000 0.69 +0.8
411,454 + BWI76_RS02000 0.69 +0.2
411,454 + BWI76_RS02000 0.69 -0.4
411,454 + BWI76_RS02000 0.69 +0.4
411,454 + BWI76_RS02000 0.69 -0.8
411,462 - BWI76_RS02000 0.71 -0.0
411,462 - BWI76_RS02000 0.71 -1.3
411,462 - BWI76_RS02000 0.71 -1.2
411,462 - BWI76_RS02000 0.71 +0.8
411,699 + -0.0
411,699 + +0.5
411,699 + -0.4
411,699 + +0.0
411,702 - +0.7
411,707 - +0.0
411,707 - +1.3
411,707 - -0.8
411,707 - +0.3
411,727 + +1.8
411,864 - -1.4
411,943 - BWI76_RS02005 0.13 +0.2
411,951 - BWI76_RS02005 0.14 +0.3
411,953 + BWI76_RS02005 0.15 +0.9
411,959 + BWI76_RS02005 0.16 -0.8
412,056 + BWI76_RS02005 0.30 -0.6
412,078 + BWI76_RS02005 0.33 +0.6
412,085 - BWI76_RS02005 0.34 +0.8
412,145 + BWI76_RS02005 0.43 +1.5
412,150 + BWI76_RS02005 0.44 -0.2
412,213 - BWI76_RS02005 0.54 +0.7
412,253 + BWI76_RS02005 0.59 +0.5
412,261 - BWI76_RS02005 0.61 -1.8
412,285 - BWI76_RS02005 0.64 +0.7
412,322 + BWI76_RS02005 0.70 -0.9
412,461 - -0.9
412,463 + -0.2

Or see this region's nucleotide sequence