Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01990

Experiment: L-Tryptophan (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01980 and BWI76_RS01985 are separated by 145 nucleotidesBWI76_RS01985 and BWI76_RS01990 are separated by 544 nucleotidesBWI76_RS01990 and BWI76_RS01995 overlap by 16 nucleotidesBWI76_RS01995 and BWI76_RS02000 are separated by 84 nucleotides BWI76_RS01980: BWI76_RS01980 - anti-adapter protein IraM, at 407,774 to 408,163 _RS01980 BWI76_RS01985: BWI76_RS01985 - membrane protein, at 408,309 to 408,590 _RS01985 BWI76_RS01990: BWI76_RS01990 - cyclic diguanylate phosphodiesterase, at 409,135 to 410,739 _RS01990 BWI76_RS01995: BWI76_RS01995 - AraC family transcriptional regulator, at 410,724 to 411,053 _RS01995 BWI76_RS02000: BWI76_RS02000 - transcriptional regulator, at 411,138 to 411,596 _RS02000 Position (kb) 409 410 411Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 408.166 kb on - strandat 408.200 kb on - strandat 408.208 kb on + strandat 408.208 kb on + strandat 408.247 kb on + strandat 408.255 kb on - strandat 408.255 kb on - strandat 408.265 kb on - strandat 408.283 kb on - strandat 408.394 kb on + strand, within BWI76_RS01985at 408.449 kb on + strand, within BWI76_RS01985at 408.449 kb on + strand, within BWI76_RS01985at 408.457 kb on - strand, within BWI76_RS01985at 408.457 kb on - strand, within BWI76_RS01985at 408.470 kb on + strand, within BWI76_RS01985at 408.498 kb on - strandat 408.528 kb on + strand, within BWI76_RS01985at 408.528 kb on + strand, within BWI76_RS01985at 408.528 kb on + strand, within BWI76_RS01985at 408.541 kb on - strand, within BWI76_RS01985at 408.546 kb on - strand, within BWI76_RS01985at 408.553 kb on - strand, within BWI76_RS01985at 408.563 kb on - strandat 408.592 kb on + strandat 408.654 kb on + strandat 408.772 kb on - strandat 408.880 kb on + strandat 408.939 kb on + strandat 409.009 kb on - strandat 409.030 kb on + strandat 409.035 kb on + strandat 409.038 kb on - strandat 409.043 kb on - strandat 409.049 kb on + strandat 409.049 kb on + strandat 409.062 kb on - strandat 409.062 kb on - strandat 409.088 kb on + strandat 409.088 kb on + strandat 409.088 kb on + strandat 409.088 kb on + strandat 409.096 kb on - strandat 409.102 kb on + strandat 409.102 kb on + strandat 409.102 kb on + strandat 409.102 kb on + strandat 409.110 kb on - strandat 409.110 kb on - strandat 409.110 kb on - strandat 409.110 kb on - strandat 409.110 kb on - strandat 409.137 kb on + strandat 409.180 kb on + strandat 409.180 kb on + strandat 409.184 kb on + strandat 409.194 kb on - strandat 409.204 kb on + strandat 409.217 kb on + strandat 409.222 kb on + strandat 409.235 kb on - strandat 409.329 kb on - strand, within BWI76_RS01990at 409.374 kb on + strand, within BWI76_RS01990at 409.381 kb on - strand, within BWI76_RS01990at 409.404 kb on + strand, within BWI76_RS01990at 409.412 kb on - strand, within BWI76_RS01990at 409.412 kb on - strand, within BWI76_RS01990at 409.417 kb on + strand, within BWI76_RS01990at 409.571 kb on + strand, within BWI76_RS01990at 409.571 kb on + strand, within BWI76_RS01990at 409.579 kb on - strand, within BWI76_RS01990at 409.579 kb on - strand, within BWI76_RS01990at 409.579 kb on - strand, within BWI76_RS01990at 409.579 kb on - strand, within BWI76_RS01990at 409.579 kb on - strand, within BWI76_RS01990at 409.612 kb on + strand, within BWI76_RS01990at 409.614 kb on + strand, within BWI76_RS01990at 409.616 kb on + strand, within BWI76_RS01990at 409.620 kb on - strand, within BWI76_RS01990at 409.622 kb on - strand, within BWI76_RS01990at 409.717 kb on + strand, within BWI76_RS01990at 409.720 kb on - strand, within BWI76_RS01990at 409.725 kb on - strand, within BWI76_RS01990at 409.726 kb on + strand, within BWI76_RS01990at 409.734 kb on - strand, within BWI76_RS01990at 409.824 kb on - strand, within BWI76_RS01990at 409.864 kb on + strand, within BWI76_RS01990at 409.864 kb on + strand, within BWI76_RS01990at 409.864 kb on + strand, within BWI76_RS01990at 409.865 kb on + strand, within BWI76_RS01990at 409.872 kb on - strand, within BWI76_RS01990at 409.887 kb on + strand, within BWI76_RS01990at 409.887 kb on + strand, within BWI76_RS01990at 409.933 kb on + strand, within BWI76_RS01990at 409.945 kb on + strand, within BWI76_RS01990at 409.974 kb on - strand, within BWI76_RS01990at 409.997 kb on + strand, within BWI76_RS01990at 410.141 kb on - strand, within BWI76_RS01990at 410.142 kb on - strand, within BWI76_RS01990at 410.279 kb on - strand, within BWI76_RS01990at 410.291 kb on + strand, within BWI76_RS01990at 410.413 kb on + strand, within BWI76_RS01990at 410.422 kb on + strand, within BWI76_RS01990at 410.430 kb on - strand, within BWI76_RS01990at 410.625 kb on - strandat 410.915 kb on - strand, within BWI76_RS01995at 411.091 kb on - strandat 411.314 kb on + strand, within BWI76_RS02000at 411.385 kb on + strand, within BWI76_RS02000at 411.393 kb on - strand, within BWI76_RS02000at 411.393 kb on - strand, within BWI76_RS02000at 411.454 kb on + strand, within BWI76_RS02000at 411.454 kb on + strand, within BWI76_RS02000at 411.454 kb on + strand, within BWI76_RS02000at 411.454 kb on + strand, within BWI76_RS02000at 411.454 kb on + strand, within BWI76_RS02000at 411.462 kb on - strand, within BWI76_RS02000at 411.462 kb on - strand, within BWI76_RS02000at 411.462 kb on - strand, within BWI76_RS02000at 411.462 kb on - strand, within BWI76_RS02000at 411.699 kb on + strandat 411.699 kb on + strandat 411.699 kb on + strandat 411.699 kb on + strandat 411.702 kb on - strandat 411.707 kb on - strandat 411.707 kb on - strandat 411.707 kb on - strandat 411.707 kb on - strandat 411.727 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Tryptophan (N)
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408,166 - +0.3
408,200 - +0.2
408,208 + +0.4
408,208 + -0.4
408,247 + -1.0
408,255 - -0.8
408,255 - -1.8
408,265 - -0.2
408,283 - -0.5
408,394 + BWI76_RS01985 0.30 +0.5
408,449 + BWI76_RS01985 0.50 +0.0
408,449 + BWI76_RS01985 0.50 +0.1
408,457 - BWI76_RS01985 0.52 -0.2
408,457 - BWI76_RS01985 0.52 -0.1
408,470 + BWI76_RS01985 0.57 +0.0
408,498 - +2.7
408,528 + BWI76_RS01985 0.78 +0.0
408,528 + BWI76_RS01985 0.78 +0.1
408,528 + BWI76_RS01985 0.78 -1.1
408,541 - BWI76_RS01985 0.82 +0.7
408,546 - BWI76_RS01985 0.84 -0.8
408,553 - BWI76_RS01985 0.87 +0.5
408,563 - -0.5
408,592 + +1.3
408,654 + +0.7
408,772 - +1.3
408,880 + +1.9
408,939 + -0.1
409,009 - +0.9
409,030 + -0.4
409,035 + +0.2
409,038 - -0.7
409,043 - +0.1
409,049 + +0.4
409,049 + +1.9
409,062 - -2.4
409,062 - -1.2
409,088 + +0.2
409,088 + +1.2
409,088 + +0.4
409,088 + +0.3
409,096 - -0.2
409,102 + -0.5
409,102 + +1.3
409,102 + +0.1
409,102 + +0.2
409,110 - -2.1
409,110 - +1.8
409,110 - -0.7
409,110 - -0.7
409,110 - -1.7
409,137 + +0.0
409,180 + -0.8
409,180 + +0.7
409,184 + -0.1
409,194 - -2.0
409,204 + -1.4
409,217 + +0.0
409,222 + +0.2
409,235 - -0.5
409,329 - BWI76_RS01990 0.12 +0.2
409,374 + BWI76_RS01990 0.15 -1.2
409,381 - BWI76_RS01990 0.15 -0.9
409,404 + BWI76_RS01990 0.17 +0.9
409,412 - BWI76_RS01990 0.17 -2.6
409,412 - BWI76_RS01990 0.17 -1.2
409,417 + BWI76_RS01990 0.18 -1.2
409,571 + BWI76_RS01990 0.27 -3.5
409,571 + BWI76_RS01990 0.27 -0.7
409,579 - BWI76_RS01990 0.28 -0.5
409,579 - BWI76_RS01990 0.28 -0.9
409,579 - BWI76_RS01990 0.28 -0.9
409,579 - BWI76_RS01990 0.28 -1.3
409,579 - BWI76_RS01990 0.28 -0.7
409,612 + BWI76_RS01990 0.30 -0.2
409,614 + BWI76_RS01990 0.30 -1.1
409,616 + BWI76_RS01990 0.30 +1.4
409,620 - BWI76_RS01990 0.30 -2.9
409,622 - BWI76_RS01990 0.30 -1.3
409,717 + BWI76_RS01990 0.36 -0.7
409,720 - BWI76_RS01990 0.36 -1.6
409,725 - BWI76_RS01990 0.37 -0.4
409,726 + BWI76_RS01990 0.37 +1.0
409,734 - BWI76_RS01990 0.37 +0.5
409,824 - BWI76_RS01990 0.43 -0.4
409,864 + BWI76_RS01990 0.45 -0.0
409,864 + BWI76_RS01990 0.45 -0.9
409,864 + BWI76_RS01990 0.45 -1.1
409,865 + BWI76_RS01990 0.45 +0.0
409,872 - BWI76_RS01990 0.46 +0.7
409,887 + BWI76_RS01990 0.47 -0.7
409,887 + BWI76_RS01990 0.47 +0.6
409,933 + BWI76_RS01990 0.50 +0.3
409,945 + BWI76_RS01990 0.50 -0.5
409,974 - BWI76_RS01990 0.52 -2.8
409,997 + BWI76_RS01990 0.54 +2.0
410,141 - BWI76_RS01990 0.63 -1.6
410,142 - BWI76_RS01990 0.63 -2.4
410,279 - BWI76_RS01990 0.71 -0.1
410,291 + BWI76_RS01990 0.72 -1.0
410,413 + BWI76_RS01990 0.80 -0.4
410,422 + BWI76_RS01990 0.80 -2.2
410,430 - BWI76_RS01990 0.81 -0.8
410,625 - -0.9
410,915 - BWI76_RS01995 0.58 -1.3
411,091 - +0.8
411,314 + BWI76_RS02000 0.38 -0.4
411,385 + BWI76_RS02000 0.54 +0.6
411,393 - BWI76_RS02000 0.56 +0.2
411,393 - BWI76_RS02000 0.56 -0.2
411,454 + BWI76_RS02000 0.69 +0.8
411,454 + BWI76_RS02000 0.69 +0.2
411,454 + BWI76_RS02000 0.69 -0.4
411,454 + BWI76_RS02000 0.69 +0.4
411,454 + BWI76_RS02000 0.69 -0.8
411,462 - BWI76_RS02000 0.71 -0.0
411,462 - BWI76_RS02000 0.71 -1.3
411,462 - BWI76_RS02000 0.71 -1.2
411,462 - BWI76_RS02000 0.71 +0.8
411,699 + -0.0
411,699 + +0.5
411,699 + -0.4
411,699 + +0.0
411,702 - +0.7
411,707 - +0.0
411,707 - +1.3
411,707 - -0.8
411,707 - +0.3
411,727 + +1.8

Or see this region's nucleotide sequence