Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25005

Experiment: L-tyrosine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS24995 and BWI76_RS25000 are separated by 11 nucleotidesBWI76_RS25000 and BWI76_RS25005 overlap by 8 nucleotidesBWI76_RS25005 and BWI76_RS25010 overlap by 1 nucleotidesBWI76_RS25010 and BWI76_RS25015 are separated by 17 nucleotides BWI76_RS24995: BWI76_RS24995 - malonate decarboxylase subunit alpha, at 5,205,172 to 5,206,839 _RS24995 BWI76_RS25000: BWI76_RS25000 - malonate decarboxylase acyl carrier protein, at 5,206,851 to 5,207,183 _RS25000 BWI76_RS25005: BWI76_RS25005 - biotin-independent malonate decarboxylase subunit beta, at 5,207,176 to 5,208,105 _RS25005 BWI76_RS25010: BWI76_RS25010 - malonyl-S-acyl carrier protein--biotin carboxyltransferase, at 5,208,105 to 5,208,986 _RS25010 BWI76_RS25015: BWI76_RS25015 - hypothetical protein, at 5,209,004 to 5,210,107 _RS25015 Position (kb) 5207 5208 5209Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 5206.192 kb on + strand, within BWI76_RS24995at 5206.376 kb on - strand, within BWI76_RS24995at 5206.641 kb on - strand, within BWI76_RS24995at 5206.684 kb on + strandat 5206.806 kb on + strandat 5206.812 kb on - strandat 5206.854 kb on + strandat 5207.092 kb on - strand, within BWI76_RS25000at 5207.131 kb on + strand, within BWI76_RS25000at 5207.151 kb on - strandat 5207.296 kb on + strand, within BWI76_RS25005at 5207.301 kb on + strand, within BWI76_RS25005at 5207.339 kb on + strand, within BWI76_RS25005at 5207.347 kb on - strand, within BWI76_RS25005at 5207.549 kb on - strand, within BWI76_RS25005at 5207.650 kb on - strand, within BWI76_RS25005at 5208.002 kb on + strand, within BWI76_RS25005at 5208.195 kb on - strand, within BWI76_RS25010at 5208.214 kb on + strand, within BWI76_RS25010at 5208.235 kb on - strand, within BWI76_RS25010at 5208.242 kb on + strand, within BWI76_RS25010at 5208.498 kb on - strand, within BWI76_RS25010at 5208.576 kb on + strand, within BWI76_RS25010at 5208.651 kb on + strand, within BWI76_RS25010at 5208.763 kb on - strand, within BWI76_RS25010at 5208.784 kb on - strand, within BWI76_RS25010at 5208.868 kb on + strand, within BWI76_RS25010at 5208.870 kb on + strand, within BWI76_RS25010at 5208.986 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-tyrosine (N)
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5,206,192 + BWI76_RS24995 0.61 +0.6
5,206,376 - BWI76_RS24995 0.72 -0.4
5,206,641 - BWI76_RS24995 0.88 +0.2
5,206,684 + -1.4
5,206,806 + +0.1
5,206,812 - -0.1
5,206,854 + +0.1
5,207,092 - BWI76_RS25000 0.72 +0.4
5,207,131 + BWI76_RS25000 0.84 -2.0
5,207,151 - +0.2
5,207,296 + BWI76_RS25005 0.13 +0.3
5,207,301 + BWI76_RS25005 0.13 +0.2
5,207,339 + BWI76_RS25005 0.18 -1.5
5,207,347 - BWI76_RS25005 0.18 +1.4
5,207,549 - BWI76_RS25005 0.40 +2.0
5,207,650 - BWI76_RS25005 0.51 +1.1
5,208,002 + BWI76_RS25005 0.89 +0.1
5,208,195 - BWI76_RS25010 0.10 +0.4
5,208,214 + BWI76_RS25010 0.12 +0.4
5,208,235 - BWI76_RS25010 0.15 -1.7
5,208,242 + BWI76_RS25010 0.16 -1.0
5,208,498 - BWI76_RS25010 0.45 -0.4
5,208,576 + BWI76_RS25010 0.53 +1.2
5,208,651 + BWI76_RS25010 0.62 +0.5
5,208,763 - BWI76_RS25010 0.75 -0.1
5,208,784 - BWI76_RS25010 0.77 -3.0
5,208,868 + BWI76_RS25010 0.87 -0.9
5,208,870 + BWI76_RS25010 0.87 -3.4
5,208,986 + +0.3

Or see this region's nucleotide sequence