Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01375

Experiment: L-tyrosine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01365 and BWI76_RS01370 are separated by 1 nucleotidesBWI76_RS01370 and BWI76_RS01375 are separated by 64 nucleotidesBWI76_RS01375 and BWI76_RS01380 overlap by 1 nucleotidesBWI76_RS01380 and BWI76_RS01385 are separated by 41 nucleotides BWI76_RS01365: BWI76_RS01365 - multifunctional fatty acid oxidation complex subunit alpha, at 278,637 to 280,826 _RS01365 BWI76_RS01370: BWI76_RS01370 - 3-hydroxyacyl-CoA dehydrogenase, at 280,828 to 280,953 _RS01370 BWI76_RS01375: BWI76_RS01375 - Xaa-Pro dipeptidase, at 281,018 to 282,349 _RS01375 BWI76_RS01380: BWI76_RS01380 - YigZ family protein, at 282,349 to 282,963 _RS01380 BWI76_RS01385: BWI76_RS01385 - potassium transporter, at 283,005 to 284,456 _RS01385 Position (kb) 281 282 283Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 280.157 kb on + strand, within BWI76_RS01365at 280.192 kb on + strand, within BWI76_RS01365at 280.298 kb on + strand, within BWI76_RS01365at 280.298 kb on + strand, within BWI76_RS01365at 280.311 kb on + strand, within BWI76_RS01365at 280.319 kb on - strand, within BWI76_RS01365at 280.485 kb on - strand, within BWI76_RS01365at 280.495 kb on - strand, within BWI76_RS01365at 280.583 kb on - strand, within BWI76_RS01365at 280.601 kb on - strand, within BWI76_RS01365at 280.690 kb on + strandat 280.710 kb on - strandat 280.724 kb on - strandat 280.805 kb on + strandat 280.951 kb on - strandat 280.971 kb on - strandat 281.092 kb on + strandat 281.121 kb on + strandat 281.121 kb on + strandat 281.204 kb on + strand, within BWI76_RS01375at 281.289 kb on + strand, within BWI76_RS01375at 281.342 kb on - strand, within BWI76_RS01375at 281.457 kb on + strand, within BWI76_RS01375at 281.508 kb on + strand, within BWI76_RS01375at 281.656 kb on - strand, within BWI76_RS01375at 281.686 kb on + strand, within BWI76_RS01375at 281.698 kb on + strand, within BWI76_RS01375at 281.711 kb on - strand, within BWI76_RS01375at 281.737 kb on + strand, within BWI76_RS01375at 281.869 kb on + strand, within BWI76_RS01375at 281.869 kb on + strand, within BWI76_RS01375at 281.869 kb on + strand, within BWI76_RS01375at 281.869 kb on + strand, within BWI76_RS01375at 281.877 kb on - strand, within BWI76_RS01375at 281.902 kb on + strand, within BWI76_RS01375at 281.959 kb on - strand, within BWI76_RS01375at 282.032 kb on - strand, within BWI76_RS01375at 282.032 kb on - strand, within BWI76_RS01375at 282.076 kb on + strand, within BWI76_RS01375at 282.095 kb on - strand, within BWI76_RS01375at 282.095 kb on - strand, within BWI76_RS01375at 282.099 kb on - strand, within BWI76_RS01375at 282.100 kb on - strand, within BWI76_RS01375at 282.220 kb on + strandat 282.230 kb on + strandat 282.479 kb on - strand, within BWI76_RS01380at 282.591 kb on + strand, within BWI76_RS01380at 282.719 kb on + strand, within BWI76_RS01380at 282.720 kb on - strand, within BWI76_RS01380at 283.237 kb on - strand, within BWI76_RS01385at 283.264 kb on + strand, within BWI76_RS01385

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Per-strain Table

Position Strand Gene LocusTag Fraction L-tyrosine (N)
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280,157 + BWI76_RS01365 0.69 +0.4
280,192 + BWI76_RS01365 0.71 +0.7
280,298 + BWI76_RS01365 0.76 -0.2
280,298 + BWI76_RS01365 0.76 +0.8
280,311 + BWI76_RS01365 0.76 +0.4
280,319 - BWI76_RS01365 0.77 -0.1
280,485 - BWI76_RS01365 0.84 -2.9
280,495 - BWI76_RS01365 0.85 -0.4
280,583 - BWI76_RS01365 0.89 -0.6
280,601 - BWI76_RS01365 0.90 -0.5
280,690 + -0.1
280,710 - +2.4
280,724 - -0.4
280,805 + -0.6
280,951 - +0.8
280,971 - -0.3
281,092 + +2.4
281,121 + -0.7
281,121 + -0.6
281,204 + BWI76_RS01375 0.14 +1.7
281,289 + BWI76_RS01375 0.20 +1.1
281,342 - BWI76_RS01375 0.24 -0.2
281,457 + BWI76_RS01375 0.33 -1.3
281,508 + BWI76_RS01375 0.37 -3.1
281,656 - BWI76_RS01375 0.48 +0.4
281,686 + BWI76_RS01375 0.50 +0.3
281,698 + BWI76_RS01375 0.51 +0.8
281,711 - BWI76_RS01375 0.52 -1.2
281,737 + BWI76_RS01375 0.54 -1.2
281,869 + BWI76_RS01375 0.64 +1.3
281,869 + BWI76_RS01375 0.64 +0.1
281,869 + BWI76_RS01375 0.64 +0.3
281,869 + BWI76_RS01375 0.64 +0.1
281,877 - BWI76_RS01375 0.64 +1.5
281,902 + BWI76_RS01375 0.66 +1.0
281,959 - BWI76_RS01375 0.71 +1.0
282,032 - BWI76_RS01375 0.76 +1.8
282,032 - BWI76_RS01375 0.76 +0.1
282,076 + BWI76_RS01375 0.79 -2.4
282,095 - BWI76_RS01375 0.81 -0.3
282,095 - BWI76_RS01375 0.81 -3.2
282,099 - BWI76_RS01375 0.81 +1.1
282,100 - BWI76_RS01375 0.81 -0.9
282,220 + +1.2
282,230 + +1.1
282,479 - BWI76_RS01380 0.21 -0.8
282,591 + BWI76_RS01380 0.39 +1.6
282,719 + BWI76_RS01380 0.60 +2.0
282,720 - BWI76_RS01380 0.60 -1.3
283,237 - BWI76_RS01385 0.16 -1.4
283,264 + BWI76_RS01385 0.18 -2.0

Or see this region's nucleotide sequence