Experiment: Succinic Acid; Light intensity 9 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2485 and Rru_A2486 overlap by 8 nucleotides Rru_A2486 and Rru_A2487 overlap by 4 nucleotides Rru_A2487 and Rru_A2488 are separated by 2 nucleotides
Rru_A2485: Rru_A2485 - Alpha,alpha-trehalose-phosphate synthase (UDP-forming) (NCBI), at 2,888,640 to 2,890,022
_A2485
Rru_A2486: Rru_A2486 - Glucokinase (NCBI), at 2,890,015 to 2,890,989
_A2486
Rru_A2487: Rru_A2487 - BsuBIPstI restriction endonuclease-like (NCBI), at 2,890,986 to 2,892,236
_A2487
Rru_A2488: Rru_A2488 - Putative RNA methylase (NCBI), at 2,892,239 to 2,894,053
_A2488
Position (kb)
2890
2891
2892
2893 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2890.162 kb on + strand, within Rru_A2486 at 2890.162 kb on + strand, within Rru_A2486 at 2890.162 kb on + strand, within Rru_A2486 at 2890.753 kb on + strand, within Rru_A2486 at 2890.831 kb on - strand, within Rru_A2486 at 2890.909 kb on + strand at 2890.910 kb on - strand at 2890.910 kb on - strand at 2890.910 kb on - strand at 2890.987 kb on + strand at 2890.987 kb on + strand at 2890.987 kb on + strand at 2891.132 kb on - strand, within Rru_A2487 at 2891.304 kb on - strand, within Rru_A2487 at 2891.663 kb on - strand, within Rru_A2487 at 2891.828 kb on - strand, within Rru_A2487 at 2891.931 kb on - strand, within Rru_A2487 at 2891.932 kb on + strand, within Rru_A2487 at 2891.932 kb on + strand, within Rru_A2487 at 2891.933 kb on - strand, within Rru_A2487 at 2891.933 kb on - strand, within Rru_A2487 at 2891.933 kb on - strand, within Rru_A2487 at 2891.977 kb on + strand, within Rru_A2487 at 2891.978 kb on - strand, within Rru_A2487 at 2891.978 kb on - strand, within Rru_A2487 at 2891.978 kb on - strand, within Rru_A2487 at 2892.188 kb on + strand at 2892.188 kb on + strand at 2892.189 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 9 uM remove 2,890,162 + Rru_A2486 0.15 +0.9 2,890,162 + Rru_A2486 0.15 +2.4 2,890,162 + Rru_A2486 0.15 +1.3 2,890,753 + Rru_A2486 0.76 -0.5 2,890,831 - Rru_A2486 0.84 +1.8 2,890,909 + +2.0 2,890,910 - +1.1 2,890,910 - +1.5 2,890,910 - +1.5 2,890,987 + +1.3 2,890,987 + -0.0 2,890,987 + +1.3 2,891,132 - Rru_A2487 0.12 -1.0 2,891,304 - Rru_A2487 0.25 +0.9 2,891,663 - Rru_A2487 0.54 -0.7 2,891,828 - Rru_A2487 0.67 -0.0 2,891,931 - Rru_A2487 0.76 +1.5 2,891,932 + Rru_A2487 0.76 +1.3 2,891,932 + Rru_A2487 0.76 -0.0 2,891,933 - Rru_A2487 0.76 -1.0 2,891,933 - Rru_A2487 0.76 -0.0 2,891,933 - Rru_A2487 0.76 +0.1 2,891,977 + Rru_A2487 0.79 -0.0 2,891,978 - Rru_A2487 0.79 +3.1 2,891,978 - Rru_A2487 0.79 -1.6 2,891,978 - Rru_A2487 0.79 +0.9 2,892,188 + +1.2 2,892,188 + -0.0 2,892,189 - +0.7
Or see this region's nucleotide sequence