Experiment: Succinic Acid; Light intensity 9 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2017 and Rru_A2018 are separated by 81 nucleotides Rru_A2018 and Rru_A2019 are separated by 188 nucleotides Rru_A2019 and Rru_A2020 are separated by 13 nucleotides Rru_A2020 and Rru_A2021 are separated by 443 nucleotides
Rru_A2017: Rru_A2017 - hypothetical protein (NCBI), at 2,331,798 to 2,332,040
_A2017
Rru_A2018: Rru_A2018 - hypothetical protein (NCBI), at 2,332,122 to 2,332,511
_A2018
Rru_A2019: Rru_A2019 - Plasmid maintenance system antidote protein (NCBI), at 2,332,700 to 2,332,999
_A2019
Rru_A2020: Rru_A2020 - Plasmid maintenance system killer (NCBI), at 2,333,013 to 2,333,294
_A2020
Rru_A2021: Rru_A2021 - Endonuclease/exonuclease/phosphatase (NCBI), at 2,333,738 to 2,334,478
_A2021
Position (kb)
2333
2334 Strain fitness (log2 ratio)
-1
0
1
2 at 2332.029 kb on + strand at 2332.030 kb on - strand at 2332.076 kb on + strand at 2332.077 kb on - strand at 2332.077 kb on - strand at 2332.077 kb on - strand at 2332.134 kb on + strand at 2332.135 kb on - strand at 2332.170 kb on + strand, within Rru_A2018 at 2332.556 kb on - strand at 2332.613 kb on + strand at 2332.613 kb on + strand at 2333.015 kb on - strand at 2333.021 kb on - strand at 2333.220 kb on - strand, within Rru_A2020 at 2333.234 kb on + strand, within Rru_A2020 at 2333.235 kb on - strand, within Rru_A2020 at 2333.303 kb on + strand at 2333.303 kb on + strand at 2333.305 kb on + strand at 2333.336 kb on + strand at 2333.336 kb on + strand at 2333.759 kb on - strand at 2333.759 kb on - strand at 2333.814 kb on + strand, within Rru_A2021 at 2333.814 kb on + strand, within Rru_A2021 at 2333.814 kb on + strand, within Rru_A2021 at 2333.815 kb on - strand, within Rru_A2021 at 2333.815 kb on - strand, within Rru_A2021 at 2333.815 kb on - strand, within Rru_A2021 at 2334.097 kb on - strand, within Rru_A2021 at 2334.126 kb on + strand, within Rru_A2021 at 2334.126 kb on + strand, within Rru_A2021 at 2334.126 kb on + strand, within Rru_A2021 at 2334.126 kb on + strand, within Rru_A2021 at 2334.127 kb on - strand, within Rru_A2021 at 2334.127 kb on - strand, within Rru_A2021 at 2334.127 kb on - strand, within Rru_A2021 at 2334.127 kb on - strand, within Rru_A2021 at 2334.127 kb on - strand, within Rru_A2021 at 2334.219 kb on + strand, within Rru_A2021 at 2334.220 kb on - strand, within Rru_A2021 at 2334.220 kb on - strand, within Rru_A2021 at 2334.220 kb on - strand, within Rru_A2021 at 2334.256 kb on + strand, within Rru_A2021 at 2334.256 kb on + strand, within Rru_A2021 at 2334.256 kb on + strand, within Rru_A2021 at 2334.256 kb on + strand, within Rru_A2021 at 2334.257 kb on - strand, within Rru_A2021 at 2334.262 kb on + strand, within Rru_A2021 at 2334.263 kb on - strand, within Rru_A2021 at 2334.263 kb on - strand, within Rru_A2021 at 2334.263 kb on - strand, within Rru_A2021 at 2334.279 kb on + strand, within Rru_A2021 at 2334.280 kb on - strand, within Rru_A2021 at 2334.280 kb on - strand, within Rru_A2021 at 2334.280 kb on - strand, within Rru_A2021
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 9 uM remove 2,332,029 + -0.1 2,332,030 - -0.4 2,332,076 + +1.9 2,332,077 - -0.1 2,332,077 - +2.5 2,332,077 - +1.1 2,332,134 + +1.8 2,332,135 - +0.5 2,332,170 + Rru_A2018 0.12 +2.3 2,332,556 - +1.2 2,332,613 + +1.3 2,332,613 + +1.1 2,333,015 - +0.9 2,333,021 - +1.9 2,333,220 - Rru_A2020 0.73 -0.1 2,333,234 + Rru_A2020 0.78 -0.1 2,333,235 - Rru_A2020 0.79 -0.3 2,333,303 + -1.1 2,333,303 + +1.3 2,333,305 + +0.4 2,333,336 + -0.1 2,333,336 + -0.1 2,333,759 - -0.4 2,333,759 - -0.1 2,333,814 + Rru_A2021 0.10 +1.1 2,333,814 + Rru_A2021 0.10 +0.5 2,333,814 + Rru_A2021 0.10 +0.9 2,333,815 - Rru_A2021 0.10 +0.7 2,333,815 - Rru_A2021 0.10 +0.8 2,333,815 - Rru_A2021 0.10 +0.9 2,334,097 - Rru_A2021 0.48 -1.1 2,334,126 + Rru_A2021 0.52 -0.1 2,334,126 + Rru_A2021 0.52 -0.1 2,334,126 + Rru_A2021 0.52 +1.3 2,334,126 + Rru_A2021 0.52 +1.3 2,334,127 - Rru_A2021 0.52 +0.9 2,334,127 - Rru_A2021 0.52 -1.6 2,334,127 - Rru_A2021 0.52 -0.1 2,334,127 - Rru_A2021 0.52 -0.1 2,334,127 - Rru_A2021 0.52 +1.2 2,334,219 + Rru_A2021 0.65 -0.2 2,334,220 - Rru_A2021 0.65 +1.6 2,334,220 - Rru_A2021 0.65 +1.2 2,334,220 - Rru_A2021 0.65 +0.8 2,334,256 + Rru_A2021 0.70 -0.1 2,334,256 + Rru_A2021 0.70 -0.1 2,334,256 + Rru_A2021 0.70 -0.1 2,334,256 + Rru_A2021 0.70 +0.2 2,334,257 - Rru_A2021 0.70 +2.1 2,334,262 + Rru_A2021 0.71 +1.2 2,334,263 - Rru_A2021 0.71 +1.2 2,334,263 - Rru_A2021 0.71 +0.9 2,334,263 - Rru_A2021 0.71 +0.3 2,334,279 + Rru_A2021 0.73 +0.7 2,334,280 - Rru_A2021 0.73 +1.5 2,334,280 - Rru_A2021 0.73 +0.1 2,334,280 - Rru_A2021 0.73 +0.2
Or see this region's nucleotide sequence