Experiment: Succinic Acid; Light intensity 9 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2015 and Rru_A2016 overlap by 4 nucleotides Rru_A2016 and Rru_AR0038 are separated by 73 nucleotides Rru_AR0038 and Rru_A2017 are separated by 126 nucleotides Rru_A2017 and Rru_A2018 are separated by 81 nucleotides Rru_A2018 and Rru_A2019 are separated by 188 nucleotides Rru_A2019 and Rru_A2020 are separated by 13 nucleotides
Rru_A2015: Rru_A2015 - chaperone DnaJ (NCBI), at 2,330,185 to 2,331,186
_A2015
Rru_A2016: Rru_A2016 - hypothetical protein (NCBI), at 2,331,183 to 2,331,521
_A2016
Rru_AR0038: Rru_AR0038 - tRNA-Met (NCBI), at 2,331,595 to 2,331,671
_AR0038
Rru_A2017: Rru_A2017 - hypothetical protein (NCBI), at 2,331,798 to 2,332,040
_A2017
Rru_A2018: Rru_A2018 - hypothetical protein (NCBI), at 2,332,122 to 2,332,511
_A2018
Rru_A2019: Rru_A2019 - Plasmid maintenance system antidote protein (NCBI), at 2,332,700 to 2,332,999
_A2019
Rru_A2020: Rru_A2020 - Plasmid maintenance system killer (NCBI), at 2,333,013 to 2,333,294
_A2020
Position (kb)
2332
2333 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2331.175 kb on - strand at 2331.175 kb on - strand at 2331.175 kb on - strand at 2331.190 kb on + strand at 2331.190 kb on + strand at 2331.190 kb on + strand at 2331.190 kb on + strand at 2331.191 kb on - strand at 2331.191 kb on - strand at 2331.191 kb on - strand at 2331.191 kb on - strand at 2331.191 kb on - strand at 2331.191 kb on - strand at 2331.247 kb on + strand, within Rru_A2016 at 2331.248 kb on - strand, within Rru_A2016 at 2331.575 kb on + strand at 2331.676 kb on + strand at 2331.677 kb on - strand at 2331.677 kb on - strand at 2331.808 kb on + strand at 2331.809 kb on - strand at 2331.809 kb on - strand at 2331.832 kb on - strand, within Rru_A2017 at 2331.856 kb on + strand, within Rru_A2017 at 2331.867 kb on - strand, within Rru_A2017 at 2331.867 kb on - strand, within Rru_A2017 at 2331.955 kb on + strand, within Rru_A2017 at 2331.955 kb on + strand, within Rru_A2017 at 2331.956 kb on - strand, within Rru_A2017 at 2332.029 kb on + strand at 2332.030 kb on - strand at 2332.076 kb on + strand at 2332.077 kb on - strand at 2332.077 kb on - strand at 2332.077 kb on - strand at 2332.134 kb on + strand at 2332.135 kb on - strand at 2332.170 kb on + strand, within Rru_A2018 at 2332.556 kb on - strand at 2332.613 kb on + strand at 2332.613 kb on + strand at 2333.015 kb on - strand at 2333.021 kb on - strand at 2333.220 kb on - strand, within Rru_A2020 at 2333.234 kb on + strand, within Rru_A2020 at 2333.235 kb on - strand, within Rru_A2020 at 2333.303 kb on + strand at 2333.303 kb on + strand at 2333.305 kb on + strand at 2333.336 kb on + strand at 2333.336 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 9 uM remove 2,331,175 - +1.4 2,331,175 - +0.4 2,331,175 - +1.5 2,331,190 + +3.0 2,331,190 + -0.1 2,331,190 + -0.1 2,331,190 + +0.6 2,331,191 - -0.1 2,331,191 - -1.6 2,331,191 - -0.1 2,331,191 - -0.1 2,331,191 - -0.1 2,331,191 - +0.7 2,331,247 + Rru_A2016 0.19 -0.4 2,331,248 - Rru_A2016 0.19 +1.1 2,331,575 + +1.8 2,331,676 + +2.3 2,331,677 - +2.4 2,331,677 - +1.5 2,331,808 + -0.1 2,331,809 - -0.1 2,331,809 - -0.1 2,331,832 - Rru_A2017 0.14 +2.2 2,331,856 + Rru_A2017 0.24 +0.9 2,331,867 - Rru_A2017 0.28 +1.4 2,331,867 - Rru_A2017 0.28 +1.5 2,331,955 + Rru_A2017 0.65 +1.8 2,331,955 + Rru_A2017 0.65 +0.7 2,331,956 - Rru_A2017 0.65 +1.0 2,332,029 + -0.1 2,332,030 - -0.4 2,332,076 + +1.9 2,332,077 - -0.1 2,332,077 - +2.5 2,332,077 - +1.1 2,332,134 + +1.8 2,332,135 - +0.5 2,332,170 + Rru_A2018 0.12 +2.3 2,332,556 - +1.2 2,332,613 + +1.3 2,332,613 + +1.1 2,333,015 - +0.9 2,333,021 - +1.9 2,333,220 - Rru_A2020 0.73 -0.1 2,333,234 + Rru_A2020 0.78 -0.1 2,333,235 - Rru_A2020 0.79 -0.3 2,333,303 + -1.1 2,333,303 + +1.3 2,333,305 + +0.4 2,333,336 + -0.1 2,333,336 + -0.1
Or see this region's nucleotide sequence