Experiment: Succinic Acid; Light intensity 9 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1941 and Rru_A1942 overlap by 19 nucleotides Rru_A1942 and Rru_A1943 are separated by 13 nucleotides Rru_A1943 and Rru_A1944 are separated by 111 nucleotides Rru_A1944 and Rru_A1945 are separated by 156 nucleotides
Rru_A1941: Rru_A1941 - acyl-CoA carboxylase alpha chain (NCBI), at 2,248,388 to 2,250,448
_A1941
Rru_A1942: Rru_A1942 - Enoyl-CoA hydratase/isomerase (NCBI), at 2,250,430 to 2,251,257
_A1942
Rru_A1943: Rru_A1943 - Propionyl-CoA carboxylase (NCBI), at 2,251,271 to 2,252,878
_A1943
Rru_A1944: Rru_A1944 - DSBA oxidoreductase (NCBI), at 2,252,990 to 2,253,592
_A1944
Rru_A1945: Rru_A1945 - Short-chain dehydrogenase/reductase SDR (NCBI), at 2,253,749 to 2,254,432
_A1945
Position (kb)
2251
2252
2253 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2250.815 kb on + strand, within Rru_A1942 at 2250.815 kb on + strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.816 kb on - strand, within Rru_A1942 at 2250.929 kb on + strand, within Rru_A1942 at 2250.929 kb on + strand, within Rru_A1942 at 2251.401 kb on + strand at 2251.401 kb on + strand at 2251.401 kb on + strand at 2251.401 kb on + strand at 2251.402 kb on - strand at 2251.404 kb on + strand at 2251.405 kb on - strand at 2251.405 kb on - strand at 2251.425 kb on + strand at 2251.426 kb on - strand at 2251.593 kb on + strand, within Rru_A1943 at 2251.615 kb on - strand, within Rru_A1943 at 2251.615 kb on - strand, within Rru_A1943 at 2251.911 kb on + strand, within Rru_A1943 at 2251.911 kb on + strand, within Rru_A1943 at 2251.977 kb on + strand, within Rru_A1943 at 2251.977 kb on + strand, within Rru_A1943 at 2251.978 kb on - strand, within Rru_A1943 at 2251.978 kb on - strand, within Rru_A1943 at 2252.028 kb on + strand, within Rru_A1943 at 2252.028 kb on + strand, within Rru_A1943 at 2252.028 kb on + strand, within Rru_A1943 at 2252.029 kb on - strand, within Rru_A1943 at 2252.029 kb on - strand, within Rru_A1943 at 2252.029 kb on - strand, within Rru_A1943 at 2252.029 kb on - strand, within Rru_A1943 at 2252.136 kb on + strand, within Rru_A1943 at 2252.136 kb on + strand, within Rru_A1943 at 2252.137 kb on - strand, within Rru_A1943 at 2252.298 kb on + strand, within Rru_A1943 at 2252.299 kb on - strand, within Rru_A1943 at 2252.299 kb on - strand, within Rru_A1943 at 2252.299 kb on - strand, within Rru_A1943 at 2252.299 kb on - strand, within Rru_A1943 at 2252.377 kb on - strand, within Rru_A1943 at 2252.457 kb on + strand, within Rru_A1943 at 2252.527 kb on - strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.529 kb on + strand, within Rru_A1943 at 2252.530 kb on - strand, within Rru_A1943 at 2252.530 kb on - strand, within Rru_A1943 at 2252.530 kb on - strand, within Rru_A1943 at 2252.530 kb on - strand, within Rru_A1943 at 2252.530 kb on - strand, within Rru_A1943 at 2252.907 kb on - strand at 2252.991 kb on + strand at 2253.005 kb on - strand at 2253.017 kb on - strand at 2253.243 kb on + strand, within Rru_A1944 at 2253.243 kb on + strand, within Rru_A1944 at 2253.243 kb on + strand, within Rru_A1944 at 2253.267 kb on + strand, within Rru_A1944 at 2253.353 kb on + strand, within Rru_A1944 at 2253.353 kb on + strand, within Rru_A1944 at 2253.354 kb on - strand, within Rru_A1944 at 2253.354 kb on - strand, within Rru_A1944 at 2253.354 kb on - strand, within Rru_A1944 at 2253.408 kb on + strand, within Rru_A1944 at 2253.408 kb on + strand, within Rru_A1944 at 2253.409 kb on - strand, within Rru_A1944 at 2253.409 kb on - strand, within Rru_A1944 at 2253.547 kb on + strand at 2253.547 kb on + strand at 2253.547 kb on + strand at 2253.547 kb on + strand at 2253.547 kb on + strand at 2253.547 kb on + strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.548 kb on - strand at 2253.549 kb on + strand at 2253.550 kb on - strand at 2253.570 kb on + strand at 2253.645 kb on - strand at 2253.645 kb on - strand at 2253.645 kb on - strand at 2253.735 kb on - strand at 2253.750 kb on + strand at 2253.774 kb on + strand at 2253.775 kb on - strand at 2253.775 kb on - strand at 2253.775 kb on - strand at 2253.775 kb on - strand at 2253.775 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 9 uM remove 2,250,815 + Rru_A1942 0.46 +0.9 2,250,815 + Rru_A1942 0.46 +1.4 2,250,816 - Rru_A1942 0.47 +1.9 2,250,816 - Rru_A1942 0.47 +0.9 2,250,816 - Rru_A1942 0.47 +1.6 2,250,816 - Rru_A1942 0.47 +2.1 2,250,816 - Rru_A1942 0.47 -0.1 2,250,816 - Rru_A1942 0.47 +1.1 2,250,929 + Rru_A1942 0.60 -0.1 2,250,929 + Rru_A1942 0.60 +1.8 2,251,401 + -0.1 2,251,401 + -0.1 2,251,401 + -0.1 2,251,401 + +2.3 2,251,402 - +1.7 2,251,404 + +0.7 2,251,405 - -0.1 2,251,405 - +2.1 2,251,425 + +1.5 2,251,426 - -0.1 2,251,593 + Rru_A1943 0.20 +1.4 2,251,615 - Rru_A1943 0.21 -1.1 2,251,615 - Rru_A1943 0.21 -0.1 2,251,911 + Rru_A1943 0.40 +0.8 2,251,911 + Rru_A1943 0.40 +1.3 2,251,977 + Rru_A1943 0.44 -0.1 2,251,977 + Rru_A1943 0.44 -0.1 2,251,978 - Rru_A1943 0.44 +0.9 2,251,978 - Rru_A1943 0.44 +0.5 2,252,028 + Rru_A1943 0.47 +1.8 2,252,028 + Rru_A1943 0.47 -0.1 2,252,028 + Rru_A1943 0.47 -0.1 2,252,029 - Rru_A1943 0.47 +0.1 2,252,029 - Rru_A1943 0.47 +1.4 2,252,029 - Rru_A1943 0.47 -0.1 2,252,029 - Rru_A1943 0.47 -0.1 2,252,136 + Rru_A1943 0.54 -0.1 2,252,136 + Rru_A1943 0.54 -0.1 2,252,137 - Rru_A1943 0.54 -0.1 2,252,298 + Rru_A1943 0.64 +1.3 2,252,299 - Rru_A1943 0.64 +1.6 2,252,299 - Rru_A1943 0.64 -0.1 2,252,299 - Rru_A1943 0.64 +1.9 2,252,299 - Rru_A1943 0.64 -0.1 2,252,377 - Rru_A1943 0.69 +1.5 2,252,457 + Rru_A1943 0.74 +1.4 2,252,527 - Rru_A1943 0.78 +0.4 2,252,529 + Rru_A1943 0.78 -0.1 2,252,529 + Rru_A1943 0.78 -0.1 2,252,529 + Rru_A1943 0.78 -0.1 2,252,529 + Rru_A1943 0.78 +0.5 2,252,529 + Rru_A1943 0.78 +0.9 2,252,529 + Rru_A1943 0.78 +1.1 2,252,529 + Rru_A1943 0.78 +1.2 2,252,529 + Rru_A1943 0.78 -0.1 2,252,529 + Rru_A1943 0.78 +0.4 2,252,529 + Rru_A1943 0.78 +0.9 2,252,530 - Rru_A1943 0.78 +1.2 2,252,530 - Rru_A1943 0.78 +0.7 2,252,530 - Rru_A1943 0.78 -0.1 2,252,530 - Rru_A1943 0.78 +1.0 2,252,530 - Rru_A1943 0.78 -0.1 2,252,907 - +0.7 2,252,991 + +1.5 2,253,005 - +2.0 2,253,017 - +1.3 2,253,243 + Rru_A1944 0.42 +0.2 2,253,243 + Rru_A1944 0.42 -0.1 2,253,243 + Rru_A1944 0.42 +1.3 2,253,267 + Rru_A1944 0.46 +0.8 2,253,353 + Rru_A1944 0.60 +1.3 2,253,353 + Rru_A1944 0.60 +0.7 2,253,354 - Rru_A1944 0.60 +2.6 2,253,354 - Rru_A1944 0.60 +1.4 2,253,354 - Rru_A1944 0.60 +1.1 2,253,408 + Rru_A1944 0.69 +0.9 2,253,408 + Rru_A1944 0.69 +1.1 2,253,409 - Rru_A1944 0.69 +0.8 2,253,409 - Rru_A1944 0.69 +0.4 2,253,547 + +0.4 2,253,547 + +0.4 2,253,547 + -0.1 2,253,547 + -0.1 2,253,547 + +1.8 2,253,547 + -0.4 2,253,548 - +0.7 2,253,548 - -0.1 2,253,548 - -0.1 2,253,548 - +0.5 2,253,548 - -0.1 2,253,548 - +1.2 2,253,548 - +1.9 2,253,548 - +0.9 2,253,548 - +0.9 2,253,549 + -0.1 2,253,550 - +2.0 2,253,570 + +1.2 2,253,645 - -0.1 2,253,645 - -2.1 2,253,645 - -0.2 2,253,735 - -0.1 2,253,750 + +0.5 2,253,774 + -0.1 2,253,775 - -0.1 2,253,775 - +1.0 2,253,775 - -0.1 2,253,775 - +1.1 2,253,775 - -0.1
Or see this region's nucleotide sequence