Experiment: Succinic Acid; Light intensity 9 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1767 and Rru_A1768 overlap by 4 nucleotides Rru_A1768 and Rru_A1769 are separated by 25 nucleotides Rru_A1769 and Rru_A1770 overlap by 1 nucleotides
Rru_A1767: Rru_A1767 - peptidase M23B (NCBI), at 2,059,422 to 2,060,819
_A1767
Rru_A1768: Rru_A1768 - Protein-L-isoaspartate(D-aspartate) O-methyltransferase (NCBI), at 2,060,816 to 2,061,475
_A1768
Rru_A1769: Rru_A1769 - Acid phosphatase (NCBI), at 2,061,501 to 2,062,286
_A1769
Rru_A1770: Rru_A1770 - Serine--tRNA ligase (NCBI), at 2,062,286 to 2,063,557
_A1770
Position (kb)
2060
2061
2062 Strain fitness (log2 ratio)
-1
0
1
2 at 2060.413 kb on + strand, within Rru_A1767 at 2060.413 kb on + strand, within Rru_A1767 at 2060.558 kb on - strand, within Rru_A1767 at 2060.584 kb on + strand, within Rru_A1767 at 2060.675 kb on + strand, within Rru_A1767 at 2060.722 kb on - strand at 2060.724 kb on - strand at 2060.724 kb on - strand at 2060.724 kb on - strand at 2060.724 kb on - strand at 2060.724 kb on - strand at 2060.754 kb on - strand at 2060.754 kb on - strand at 2060.754 kb on - strand at 2060.756 kb on - strand at 2061.169 kb on + strand, within Rru_A1768 at 2061.170 kb on - strand, within Rru_A1768 at 2061.171 kb on + strand, within Rru_A1768 at 2061.172 kb on - strand, within Rru_A1768 at 2061.172 kb on - strand, within Rru_A1768 at 2061.339 kb on + strand, within Rru_A1768 at 2061.339 kb on + strand, within Rru_A1768 at 2061.950 kb on - strand, within Rru_A1769
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 9 uM remove 2,060,413 + Rru_A1767 0.71 -0.1 2,060,413 + Rru_A1767 0.71 -0.1 2,060,558 - Rru_A1767 0.81 -0.1 2,060,584 + Rru_A1767 0.83 -0.1 2,060,675 + Rru_A1767 0.90 -0.1 2,060,722 - -0.1 2,060,724 - -0.1 2,060,724 - -1.1 2,060,724 - -0.3 2,060,724 - -0.1 2,060,724 - -0.1 2,060,754 - +1.2 2,060,754 - +2.4 2,060,754 - +2.0 2,060,756 - +2.3 2,061,169 + Rru_A1768 0.53 +1.7 2,061,170 - Rru_A1768 0.54 +1.9 2,061,171 + Rru_A1768 0.54 +2.3 2,061,172 - Rru_A1768 0.54 +2.5 2,061,172 - Rru_A1768 0.54 +2.1 2,061,339 + Rru_A1768 0.79 +2.5 2,061,339 + Rru_A1768 0.79 +1.3 2,061,950 - Rru_A1769 0.57 -0.1
Or see this region's nucleotide sequence