Experiment: Succinic Acid; Light intensity 9 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0243 and Rru_A0244 are separated by 191 nucleotides Rru_A0244 and Rru_A0245 are separated by 166 nucleotides Rru_A0245 and Rru_A0246 overlap by 4 nucleotides
Rru_A0243: Rru_A0243 - tRNA/rRNA cytosine-C5-methylase (NCBI), at 301,475 to 302,839
_A0243
Rru_A0244: Rru_A0244 - IMP dehydrogenase (NCBI), at 303,031 to 304,494
_A0244
Rru_A0245: Rru_A0245 - Ribosomal RNA methyltransferase RrmJ/FtsJ (NCBI), at 304,661 to 305,452
_A0245
Rru_A0246: Rru_A0246 - Ppx/GppA phosphatase (NCBI), at 305,449 to 306,648
_A0246
Position (kb) 303
304
305 Strain fitness (log2 ratio) -1
0
1
2
3 at 302.033 kb on - strand, within Rru_A0243 at 302.471 kb on + strand, within Rru_A0243 at 302.471 kb on + strand, within Rru_A0243 at 303.021 kb on + strand at 303.027 kb on + strand at 303.027 kb on + strand at 303.027 kb on + strand at 303.028 kb on - strand at 303.028 kb on - strand at 303.028 kb on - strand at 303.028 kb on - strand at 303.028 kb on - strand at 303.032 kb on + strand at 303.032 kb on + strand at 303.032 kb on + strand at 303.033 kb on - strand at 303.033 kb on - strand at 303.033 kb on - strand at 304.512 kb on - strand at 304.662 kb on + strand at 304.663 kb on - strand at 304.663 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 9 uM remove 302,033 - Rru_A0243 0.41 +0.9 302,471 + Rru_A0243 0.73 -0.0 302,471 + Rru_A0243 0.73 +0.8 303,021 + -1.0 303,027 + +1.7 303,027 + +2.0 303,027 + +1.3 303,028 - +1.2 303,028 - +3.3 303,028 - -0.0 303,028 - -0.0 303,028 - -1.0 303,032 + +1.6 303,032 + +0.9 303,032 + +1.8 303,033 - +0.5 303,033 - +1.0 303,033 - -0.0 304,512 - -0.0 304,662 + -0.0 304,663 - +1.4 304,663 - +0.8
Or see this region's nucleotide sequence