Experiment: Succinic Acid; Light intensity 9 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A0015 and Rru_AR0001 are separated by 163 nucleotides Rru_AR0001 and Rru_A0016 are separated by 253 nucleotides Rru_A0016 and Rru_A0017 are separated by 114 nucleotides
Rru_A0015: Rru_A0015 - 3'-5' exonuclease (NCBI), at 16,232 to 16,852
_A0015
Rru_AR0001: Rru_AR0001 - tRNA-Leu (NCBI), at 17,016 to 17,102
_AR0001
Rru_A0016: Rru_A0016 - 3-beta-hydroxy-delta(5)-steroid dehydrogenase (NCBI), at 17,356 to 18,378
_A0016
Rru_A0017: Rru_A0017 - Bacitracin resistance protein BacA (NCBI), at 18,493 to 19,341
_A0017
Position (kb) 17
18
19 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 17.001 kb on + strand at 17.006 kb on + strand at 17.007 kb on - strand at 17.261 kb on + strand at 17.261 kb on + strand at 17.262 kb on - strand at 17.262 kb on - strand at 17.262 kb on - strand at 17.262 kb on - strand at 17.262 kb on - strand at 17.741 kb on - strand, within Rru_A0016 at 17.742 kb on + strand, within Rru_A0016 at 17.742 kb on + strand, within Rru_A0016 at 17.742 kb on + strand, within Rru_A0016 at 17.743 kb on - strand, within Rru_A0016 at 17.743 kb on - strand, within Rru_A0016 at 17.888 kb on - strand, within Rru_A0016 at 17.977 kb on + strand, within Rru_A0016 at 17.977 kb on + strand, within Rru_A0016 at 17.978 kb on - strand, within Rru_A0016 at 17.978 kb on - strand, within Rru_A0016 at 17.978 kb on - strand, within Rru_A0016 at 17.978 kb on - strand, within Rru_A0016 at 17.978 kb on - strand, within Rru_A0016 at 18.043 kb on + strand, within Rru_A0016 at 18.043 kb on + strand, within Rru_A0016 at 18.044 kb on - strand, within Rru_A0016 at 18.067 kb on + strand, within Rru_A0016 at 18.307 kb on + strand at 18.307 kb on + strand at 18.307 kb on + strand at 18.307 kb on + strand at 18.307 kb on + strand at 18.308 kb on - strand at 18.308 kb on - strand at 18.308 kb on - strand at 18.308 kb on - strand at 18.308 kb on - strand at 18.502 kb on + strand at 18.503 kb on - strand at 18.503 kb on - strand at 18.503 kb on - strand at 18.503 kb on - strand at 18.530 kb on + strand at 18.531 kb on - strand at 18.531 kb on - strand at 18.633 kb on - strand, within Rru_A0017 at 18.788 kb on + strand, within Rru_A0017 at 18.789 kb on - strand, within Rru_A0017 at 18.793 kb on + strand, within Rru_A0017 at 18.793 kb on + strand, within Rru_A0017 at 18.794 kb on - strand, within Rru_A0017 at 18.794 kb on - strand, within Rru_A0017 at 19.151 kb on + strand, within Rru_A0017 at 19.151 kb on + strand, within Rru_A0017 at 19.152 kb on - strand, within Rru_A0017 at 19.152 kb on - strand, within Rru_A0017 at 19.201 kb on + strand, within Rru_A0017 at 19.361 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 9 uM remove 17,001 + -0.0 17,006 + -0.0 17,007 - +1.0 17,261 + +0.6 17,261 + -0.0 17,262 - +1.6 17,262 - +2.7 17,262 - +0.3 17,262 - +1.6 17,262 - +0.5 17,741 - Rru_A0016 0.38 +0.3 17,742 + Rru_A0016 0.38 +0.2 17,742 + Rru_A0016 0.38 -0.0 17,742 + Rru_A0016 0.38 -0.0 17,743 - Rru_A0016 0.38 +1.7 17,743 - Rru_A0016 0.38 +2.0 17,888 - Rru_A0016 0.52 +1.6 17,977 + Rru_A0016 0.61 -1.3 17,977 + Rru_A0016 0.61 -0.0 17,978 - Rru_A0016 0.61 +0.1 17,978 - Rru_A0016 0.61 +1.0 17,978 - Rru_A0016 0.61 -0.4 17,978 - Rru_A0016 0.61 +0.5 17,978 - Rru_A0016 0.61 +0.2 18,043 + Rru_A0016 0.67 +0.1 18,043 + Rru_A0016 0.67 -2.8 18,044 - Rru_A0016 0.67 -0.0 18,067 + Rru_A0016 0.70 -1.0 18,307 + +2.0 18,307 + -0.7 18,307 + +0.3 18,307 + +0.3 18,307 + -0.5 18,308 - -0.0 18,308 - -0.0 18,308 - +0.8 18,308 - -0.0 18,308 - -0.0 18,502 + -0.0 18,503 - +0.7 18,503 - +2.0 18,503 - -0.0 18,503 - -0.0 18,530 + +0.7 18,531 - +0.9 18,531 - +1.3 18,633 - Rru_A0017 0.16 +0.8 18,788 + Rru_A0017 0.35 -0.0 18,789 - Rru_A0017 0.35 -0.0 18,793 + Rru_A0017 0.35 -1.0 18,793 + Rru_A0017 0.35 +1.9 18,794 - Rru_A0017 0.35 +1.0 18,794 - Rru_A0017 0.35 -0.2 19,151 + Rru_A0017 0.78 -1.0 19,151 + Rru_A0017 0.78 +0.2 19,152 - Rru_A0017 0.78 -0.0 19,152 - Rru_A0017 0.78 +1.0 19,201 + Rru_A0017 0.83 -0.6 19,361 - +1.4
Or see this region's nucleotide sequence