Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04455

Experiment: L-Phenylalanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04450 and BWI76_RS04455 are separated by 19 nucleotidesBWI76_RS04455 and BWI76_RS04460 are separated by 247 nucleotides BWI76_RS04450: BWI76_RS04450 - citrate lyase subunit alpha, at 918,565 to 920,091 _RS04450 BWI76_RS04455: BWI76_RS04455 - triphosphoribosyl-dephospho-CoA synthase CitG, at 920,111 to 921,019 _RS04455 BWI76_RS04460: BWI76_RS04460 - 4-hydroxy-tetrahydrodipicolinate reductase, at 921,267 to 922,088 _RS04460 Position (kb) 920 921 922Strain fitness (log2 ratio) -3 -2 -1 0 1at 919.454 kb on + strand, within BWI76_RS04450at 919.509 kb on + strand, within BWI76_RS04450at 919.529 kb on - strand, within BWI76_RS04450at 919.639 kb on + strand, within BWI76_RS04450at 920.000 kb on - strandat 920.081 kb on - strandat 920.159 kb on - strandat 920.251 kb on + strand, within BWI76_RS04455at 920.281 kb on - strand, within BWI76_RS04455at 920.283 kb on + strand, within BWI76_RS04455at 920.350 kb on + strand, within BWI76_RS04455at 920.372 kb on - strand, within BWI76_RS04455at 920.479 kb on - strand, within BWI76_RS04455at 920.497 kb on - strand, within BWI76_RS04455at 920.689 kb on + strand, within BWI76_RS04455at 920.728 kb on + strand, within BWI76_RS04455at 920.873 kb on + strand, within BWI76_RS04455at 920.952 kb on + strandat 921.142 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Phenylalanine (N)
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919,454 + BWI76_RS04450 0.58 +0.2
919,509 + BWI76_RS04450 0.62 +1.3
919,529 - BWI76_RS04450 0.63 -0.1
919,639 + BWI76_RS04450 0.70 -1.7
920,000 - -0.4
920,081 - +0.0
920,159 - -0.4
920,251 + BWI76_RS04455 0.15 -0.9
920,281 - BWI76_RS04455 0.19 -1.8
920,283 + BWI76_RS04455 0.19 +0.0
920,350 + BWI76_RS04455 0.26 -1.4
920,372 - BWI76_RS04455 0.29 -1.0
920,479 - BWI76_RS04455 0.40 -0.7
920,497 - BWI76_RS04455 0.42 -0.4
920,689 + BWI76_RS04455 0.64 -3.4
920,728 + BWI76_RS04455 0.68 +0.4
920,873 + BWI76_RS04455 0.84 -0.6
920,952 + -1.0
921,142 - +0.2

Or see this region's nucleotide sequence