Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3480 and Shew_3481 are separated by 123 nucleotides Shew_3481 and Shew_3482 are separated by 111 nucleotides Shew_3482 and dut overlap by 4 nucleotides dut and slmA are separated by 130 nucleotides
Shew_3480: Shew_3480 - ribosomal protein L28 (RefSeq), at 4,166,357 to 4,166,593
_3480
Shew_3481: Shew_3481 - DNA repair protein RadC (RefSeq), at 4,166,717 to 4,167,418
_3481
Shew_3482: Shew_3482 - phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase (RefSeq), at 4,167,530 to 4,168,726
_3482
Shew_3483: dut - deoxyuridine 5'-triphosphate nucleotidohydrolase (RefSeq), at 4,168,723 to 4,169,181
dut
Shew_3484: slmA - nucleoid occlusion protein (RefSeq), at 4,169,312 to 4,169,905
slmA
Position (kb)
4167
4168
4169 Strain fitness (log2 ratio)
-2
-1
0
1 at 4166.983 kb on + strand, within Shew_3481 at 4166.993 kb on + strand, within Shew_3481 at 4166.995 kb on + strand, within Shew_3481 at 4167.017 kb on + strand, within Shew_3481 at 4167.215 kb on - strand, within Shew_3481 at 4167.331 kb on - strand, within Shew_3481 at 4167.408 kb on - strand at 4167.432 kb on - strand at 4167.488 kb on + strand at 4168.811 kb on + strand, within dut at 4168.838 kb on + strand, within dut at 4169.174 kb on - strand at 4169.256 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 4,166,983 + Shew_3481 0.38 -0.9 4,166,993 + Shew_3481 0.39 -0.4 4,166,995 + Shew_3481 0.40 -0.1 4,167,017 + Shew_3481 0.43 -0.6 4,167,215 - Shew_3481 0.71 -0.8 4,167,331 - Shew_3481 0.87 -1.4 4,167,408 - +0.6 4,167,432 - -0.0 4,167,488 + -0.6 4,168,811 + dut Shew_3483 0.19 -2.6 4,168,838 + dut Shew_3483 0.25 -2.2 4,169,174 - -1.3 4,169,256 + -0.6
Or see this region's nucleotide sequence