Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1536 and Shew_1537 are separated by 321 nucleotides Shew_1537 and Shew_1538 are separated by 176 nucleotides Shew_1538 and Shew_1539 are separated by 102 nucleotides
Shew_1536: Shew_1536 - putative chloramphenicol acetyltransferase (RefSeq), at 1,764,380 to 1,765,006
_1536
Shew_1537: Shew_1537 - FAD dependent oxidoreductase (RefSeq), at 1,765,328 to 1,766,617
_1537
Shew_1538: Shew_1538 - hypothetical protein (RefSeq), at 1,766,794 to 1,767,039
_1538
Shew_1539: Shew_1539 - LysR family transcriptional regulator (RefSeq), at 1,767,142 to 1,768,011
_1539
Position (kb)
1765
1766
1767 Strain fitness (log2 ratio)
-1
0
1 at 1764.503 kb on - strand, within Shew_1536 at 1764.805 kb on + strand, within Shew_1536 at 1764.813 kb on - strand, within Shew_1536 at 1765.487 kb on - strand, within Shew_1537 at 1765.717 kb on - strand, within Shew_1537 at 1765.864 kb on - strand, within Shew_1537 at 1766.157 kb on - strand, within Shew_1537 at 1766.316 kb on + strand, within Shew_1537 at 1766.325 kb on - strand, within Shew_1537 at 1766.547 kb on + strand at 1766.568 kb on + strand at 1766.640 kb on - strand at 1766.789 kb on + strand at 1767.140 kb on + strand at 1767.147 kb on - strand at 1767.148 kb on - strand at 1767.148 kb on - strand at 1767.202 kb on + strand at 1767.470 kb on - strand, within Shew_1539 at 1767.480 kb on - strand, within Shew_1539
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,764,503 - Shew_1536 0.20 +0.2 1,764,805 + Shew_1536 0.68 -0.7 1,764,813 - Shew_1536 0.69 -0.7 1,765,487 - Shew_1537 0.12 -0.6 1,765,717 - Shew_1537 0.30 +0.1 1,765,864 - Shew_1537 0.42 +0.8 1,766,157 - Shew_1537 0.64 +0.3 1,766,316 + Shew_1537 0.77 +0.3 1,766,325 - Shew_1537 0.77 +0.2 1,766,547 + +0.7 1,766,568 + -1.0 1,766,640 - +0.3 1,766,789 + -0.0 1,767,140 + +0.7 1,767,147 - +0.5 1,767,148 - +0.2 1,767,148 - +0.0 1,767,202 + +0.3 1,767,470 - Shew_1539 0.38 +0.3 1,767,480 - Shew_1539 0.39 -0.4
Or see this region's nucleotide sequence