Experiment: Succinic Acid; Light intensity 9 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2153 and Rru_A2154 overlap by 4 nucleotides Rru_A2154 and Rru_A2155 overlap by 1 nucleotides Rru_A2155 and Rru_A2156 are separated by 442 nucleotides
Rru_A2153: Rru_A2153 - TonB-like (NCBI), at 2,501,387 to 2,502,217
_A2153
Rru_A2154: Rru_A2154 - Biopolymer transport protein ExbD/TolR (NCBI), at 2,502,214 to 2,502,681
_A2154
Rru_A2155: Rru_A2155 - MotA/TolQ/ExbB proton channel (NCBI), at 2,502,681 to 2,503,571
_A2155
Rru_A2156: Rru_A2156 - Enoyl-CoA hydratase/isomerase (NCBI), at 2,504,014 to 2,504,799
_A2156
Position (kb)
2502
2503
2504 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2501.849 kb on - strand, within Rru_A2153 at 2502.338 kb on + strand, within Rru_A2154 at 2502.365 kb on + strand, within Rru_A2154 at 2502.365 kb on + strand, within Rru_A2154 at 2502.365 kb on + strand, within Rru_A2154 at 2502.366 kb on - strand, within Rru_A2154 at 2502.366 kb on - strand, within Rru_A2154 at 2503.090 kb on + strand, within Rru_A2155 at 2503.090 kb on + strand, within Rru_A2155 at 2503.090 kb on + strand, within Rru_A2155 at 2503.090 kb on + strand, within Rru_A2155 at 2503.091 kb on - strand, within Rru_A2155 at 2503.091 kb on - strand, within Rru_A2155 at 2503.091 kb on - strand, within Rru_A2155 at 2503.566 kb on - strand at 2503.642 kb on + strand at 2503.642 kb on + strand at 2503.643 kb on - strand at 2503.710 kb on - strand at 2503.732 kb on + strand at 2503.732 kb on + strand at 2503.733 kb on - strand at 2503.733 kb on - strand at 2503.762 kb on + strand at 2503.766 kb on - strand at 2503.768 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 9 uM remove 2,501,849 - Rru_A2153 0.56 +0.7 2,502,338 + Rru_A2154 0.26 -0.1 2,502,365 + Rru_A2154 0.32 -0.1 2,502,365 + Rru_A2154 0.32 +0.6 2,502,365 + Rru_A2154 0.32 +1.0 2,502,366 - Rru_A2154 0.32 -0.1 2,502,366 - Rru_A2154 0.32 -0.1 2,503,090 + Rru_A2155 0.46 -0.1 2,503,090 + Rru_A2155 0.46 -2.4 2,503,090 + Rru_A2155 0.46 +0.1 2,503,090 + Rru_A2155 0.46 -0.1 2,503,091 - Rru_A2155 0.46 -0.1 2,503,091 - Rru_A2155 0.46 +0.9 2,503,091 - Rru_A2155 0.46 +0.8 2,503,566 - -0.1 2,503,642 + +1.5 2,503,642 + -0.1 2,503,643 - +0.3 2,503,710 - +0.6 2,503,732 + +0.7 2,503,732 + -0.1 2,503,733 - -1.1 2,503,733 - -0.1 2,503,762 + +0.7 2,503,766 - +1.8 2,503,768 + +1.3
Or see this region's nucleotide sequence