Experiment: Succinic Acid; Light intensity 9 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A2029 and Rru_A2030 are separated by 327 nucleotides Rru_A2030 and Rru_A2031 overlap by 49 nucleotides Rru_A2031 and Rru_A2032 are separated by 99 nucleotides
Rru_A2029: Rru_A2029 - Serine O-acetyltransferase (NCBI), at 2,341,796 to 2,342,560
_A2029
Rru_A2030: Rru_A2030 - hypothetical protein (NCBI), at 2,342,888 to 2,343,550
_A2030
Rru_A2031: Rru_A2031 - Protein of unknown function UPF0075 (NCBI), at 2,343,502 to 2,344,629
_A2031
Rru_A2032: Rru_A2032 - Tyrosyl-tRNA synthetase, class Ib (NCBI), at 2,344,729 to 2,345,985
_A2032
Position (kb)
2343
2344
2345 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2342.699 kb on + strand at 2342.707 kb on + strand at 2342.726 kb on + strand at 2342.726 kb on + strand at 2342.806 kb on + strand at 2342.868 kb on + strand at 2342.868 kb on + strand at 2343.548 kb on + strand at 2343.923 kb on + strand, within Rru_A2031 at 2343.923 kb on + strand, within Rru_A2031 at 2343.924 kb on - strand, within Rru_A2031 at 2343.924 kb on - strand, within Rru_A2031 at 2343.924 kb on - strand, within Rru_A2031 at 2343.924 kb on - strand, within Rru_A2031 at 2344.162 kb on + strand, within Rru_A2031 at 2344.163 kb on - strand, within Rru_A2031 at 2344.163 kb on - strand, within Rru_A2031 at 2344.169 kb on + strand, within Rru_A2031 at 2344.491 kb on - strand, within Rru_A2031 at 2344.560 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 9 uM remove 2,342,699 + +1.2 2,342,707 + -0.1 2,342,726 + +1.1 2,342,726 + +1.0 2,342,806 + -0.1 2,342,868 + -2.1 2,342,868 + +0.4 2,343,548 + +1.0 2,343,923 + Rru_A2031 0.37 -0.1 2,343,923 + Rru_A2031 0.37 -0.1 2,343,924 - Rru_A2031 0.37 +2.1 2,343,924 - Rru_A2031 0.37 +1.1 2,343,924 - Rru_A2031 0.37 +1.1 2,343,924 - Rru_A2031 0.37 -0.1 2,344,162 + Rru_A2031 0.59 -1.1 2,344,163 - Rru_A2031 0.59 -0.1 2,344,163 - Rru_A2031 0.59 +1.2 2,344,169 + Rru_A2031 0.59 -0.1 2,344,491 - Rru_A2031 0.88 +1.4 2,344,560 + -0.1
Or see this region's nucleotide sequence