Experiment: Succinic Acid; Light intensity 9 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1614 and Rru_A1615 are separated by 27 nucleotides Rru_A1615 and Rru_A1616 are separated by 61 nucleotides Rru_A1616 and Rru_A1617 are separated by 335 nucleotides
Rru_A1614: Rru_A1614 - hypothetical protein (NCBI), at 1,908,169 to 1,908,468
_A1614
Rru_A1615: Rru_A1615 - 3-mercaptopyruvate sulfurtransferase (NCBI), at 1,908,496 to 1,909,353
_A1615
Rru_A1616: Rru_A1616 - Pyridoxal-5'-phosphate-dependent enzyme, beta subunit (NCBI), at 1,909,415 to 1,910,398
_A1616
Rru_A1617: Rru_A1617 - Protein of unknown function DUF1289 (NCBI), at 1,910,734 to 1,911,645
_A1617
Position (kb)
1909
1910
1911 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1908.467 kb on - strand at 1908.467 kb on - strand at 1908.521 kb on - strand at 1908.551 kb on + strand at 1908.551 kb on + strand at 1908.552 kb on - strand at 1908.734 kb on + strand, within Rru_A1615 at 1908.734 kb on + strand, within Rru_A1615 at 1908.734 kb on + strand, within Rru_A1615 at 1908.760 kb on - strand, within Rru_A1615 at 1908.760 kb on - strand, within Rru_A1615 at 1908.891 kb on + strand, within Rru_A1615 at 1908.891 kb on + strand, within Rru_A1615 at 1908.891 kb on + strand, within Rru_A1615 at 1909.071 kb on - strand, within Rru_A1615 at 1909.210 kb on - strand, within Rru_A1615 at 1909.224 kb on - strand, within Rru_A1615 at 1909.262 kb on + strand, within Rru_A1615 at 1909.262 kb on + strand, within Rru_A1615 at 1909.263 kb on - strand, within Rru_A1615 at 1909.263 kb on - strand, within Rru_A1615 at 1909.263 kb on - strand, within Rru_A1615 at 1909.263 kb on - strand, within Rru_A1615 at 1909.263 kb on - strand, within Rru_A1615 at 1909.263 kb on - strand, within Rru_A1615 at 1909.263 kb on - strand, within Rru_A1615 at 1909.377 kb on + strand at 1909.377 kb on + strand at 1909.377 kb on + strand at 1909.377 kb on + strand at 1909.377 kb on + strand at 1909.377 kb on + strand at 1909.378 kb on - strand at 1909.378 kb on - strand at 1909.378 kb on - strand at 1909.378 kb on - strand at 1909.378 kb on - strand at 1909.378 kb on - strand at 1909.541 kb on - strand, within Rru_A1616 at 1909.589 kb on + strand, within Rru_A1616 at 1909.590 kb on - strand, within Rru_A1616 at 1909.765 kb on + strand, within Rru_A1616 at 1909.765 kb on + strand, within Rru_A1616 at 1909.766 kb on - strand, within Rru_A1616 at 1909.766 kb on - strand, within Rru_A1616 at 1909.766 kb on - strand, within Rru_A1616 at 1909.766 kb on - strand, within Rru_A1616 at 1909.766 kb on - strand, within Rru_A1616 at 1909.766 kb on - strand, within Rru_A1616 at 1909.767 kb on + strand, within Rru_A1616 at 1909.768 kb on - strand, within Rru_A1616 at 1909.768 kb on - strand, within Rru_A1616 at 1909.954 kb on + strand, within Rru_A1616 at 1910.019 kb on + strand, within Rru_A1616 at 1910.020 kb on - strand, within Rru_A1616 at 1910.020 kb on - strand, within Rru_A1616 at 1910.020 kb on - strand, within Rru_A1616 at 1910.020 kb on - strand, within Rru_A1616 at 1910.020 kb on - strand, within Rru_A1616 at 1910.035 kb on + strand, within Rru_A1616 at 1910.036 kb on - strand, within Rru_A1616 at 1910.036 kb on - strand, within Rru_A1616 at 1910.038 kb on - strand, within Rru_A1616 at 1910.038 kb on - strand, within Rru_A1616 at 1910.038 kb on - strand, within Rru_A1616 at 1910.350 kb on + strand at 1910.350 kb on + strand at 1910.351 kb on - strand at 1910.571 kb on + strand at 1910.572 kb on - strand at 1910.572 kb on - strand at 1910.572 kb on - strand at 1910.572 kb on - strand at 1910.583 kb on + strand at 1910.584 kb on - strand at 1910.612 kb on + strand at 1910.613 kb on - strand at 1910.613 kb on - strand at 1910.704 kb on + strand at 1910.761 kb on - strand at 1911.193 kb on + strand, within Rru_A1617 at 1911.193 kb on + strand, within Rru_A1617 at 1911.193 kb on + strand, within Rru_A1617 at 1911.194 kb on - strand, within Rru_A1617
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 9 uM remove 1,908,467 - +0.8 1,908,467 - +0.6 1,908,521 - -0.1 1,908,551 + -0.1 1,908,551 + +0.9 1,908,552 - -1.1 1,908,734 + Rru_A1615 0.28 +0.7 1,908,734 + Rru_A1615 0.28 +1.4 1,908,734 + Rru_A1615 0.28 -0.1 1,908,760 - Rru_A1615 0.31 -0.1 1,908,760 - Rru_A1615 0.31 -0.1 1,908,891 + Rru_A1615 0.46 -0.1 1,908,891 + Rru_A1615 0.46 +0.9 1,908,891 + Rru_A1615 0.46 +1.0 1,909,071 - Rru_A1615 0.67 -0.1 1,909,210 - Rru_A1615 0.83 -0.1 1,909,224 - Rru_A1615 0.85 +1.4 1,909,262 + Rru_A1615 0.89 +1.7 1,909,262 + Rru_A1615 0.89 +0.3 1,909,263 - Rru_A1615 0.89 +1.0 1,909,263 - Rru_A1615 0.89 -0.1 1,909,263 - Rru_A1615 0.89 +1.1 1,909,263 - Rru_A1615 0.89 +0.8 1,909,263 - Rru_A1615 0.89 +0.7 1,909,263 - Rru_A1615 0.89 +1.1 1,909,263 - Rru_A1615 0.89 +1.2 1,909,377 + +1.5 1,909,377 + +1.3 1,909,377 + +0.5 1,909,377 + -0.1 1,909,377 + +1.0 1,909,377 + +1.0 1,909,378 - -1.1 1,909,378 - +2.1 1,909,378 - +3.1 1,909,378 - -0.1 1,909,378 - +2.6 1,909,378 - -0.1 1,909,541 - Rru_A1616 0.13 +2.0 1,909,589 + Rru_A1616 0.18 +0.4 1,909,590 - Rru_A1616 0.18 +1.9 1,909,765 + Rru_A1616 0.36 +1.0 1,909,765 + Rru_A1616 0.36 -0.1 1,909,766 - Rru_A1616 0.36 -0.1 1,909,766 - Rru_A1616 0.36 -0.1 1,909,766 - Rru_A1616 0.36 -0.1 1,909,766 - Rru_A1616 0.36 -1.1 1,909,766 - Rru_A1616 0.36 +1.8 1,909,766 - Rru_A1616 0.36 -0.1 1,909,767 + Rru_A1616 0.36 -0.1 1,909,768 - Rru_A1616 0.36 +2.1 1,909,768 - Rru_A1616 0.36 +2.2 1,909,954 + Rru_A1616 0.55 +1.1 1,910,019 + Rru_A1616 0.61 -0.1 1,910,020 - Rru_A1616 0.61 -0.1 1,910,020 - Rru_A1616 0.61 +1.7 1,910,020 - Rru_A1616 0.61 -0.1 1,910,020 - Rru_A1616 0.61 +1.1 1,910,020 - Rru_A1616 0.61 -1.1 1,910,035 + Rru_A1616 0.63 +1.4 1,910,036 - Rru_A1616 0.63 -0.1 1,910,036 - Rru_A1616 0.63 +1.3 1,910,038 - Rru_A1616 0.63 +1.7 1,910,038 - Rru_A1616 0.63 -0.1 1,910,038 - Rru_A1616 0.63 +1.5 1,910,350 + -0.1 1,910,350 + -0.1 1,910,351 - +1.3 1,910,571 + -0.1 1,910,572 - +3.3 1,910,572 - +1.4 1,910,572 - -0.1 1,910,572 - -0.1 1,910,583 + +1.2 1,910,584 - +0.7 1,910,612 + +0.4 1,910,613 - -0.1 1,910,613 - -0.1 1,910,704 + -0.1 1,910,761 - +0.5 1,911,193 + Rru_A1617 0.50 -0.1 1,911,193 + Rru_A1617 0.50 -0.1 1,911,193 + Rru_A1617 0.50 -0.1 1,911,194 - Rru_A1617 0.50 +0.9
Or see this region's nucleotide sequence