Experiment: Succinic Acid; Light intensity 9 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1590 and Rru_A1591 are separated by 48 nucleotides Rru_A1591 and Rru_A1592 overlap by 4 nucleotides Rru_A1592 and Rru_A1593 are separated by 183 nucleotides
Rru_A1590: Rru_A1590 - Di-trans-poly-cis-decaprenylcistransferase (NCBI), at 1,873,813 to 1,874,547
_A1590
Rru_A1591: Rru_A1591 - Phosphatidate cytidylyltransferase (NCBI), at 1,874,596 to 1,875,402
_A1591
Rru_A1592: Rru_A1592 - 1-deoxy-D-xylulose-5-phosphate reductoisomerase (NCBI), at 1,875,399 to 1,876,601
_A1592
Rru_A1593: Rru_A1593 - Peptidase M50, putative membrane-associated zinc metallopeptidase (NCBI), at 1,876,785 to 1,877,888
_A1593
Position (kb)
1875
1876
1877 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1874.543 kb on + strand at 1874.543 kb on + strand at 1874.545 kb on + strand at 1874.545 kb on + strand at 1874.545 kb on + strand at 1874.546 kb on - strand at 1874.546 kb on - strand at 1876.627 kb on + strand at 1876.628 kb on - strand at 1876.802 kb on + strand at 1876.990 kb on - strand, within Rru_A1593 at 1877.040 kb on - strand, within Rru_A1593 at 1877.169 kb on + strand, within Rru_A1593 at 1877.169 kb on + strand, within Rru_A1593 at 1877.169 kb on + strand, within Rru_A1593 at 1877.169 kb on + strand, within Rru_A1593 at 1877.169 kb on + strand, within Rru_A1593 at 1877.169 kb on + strand, within Rru_A1593 at 1877.170 kb on - strand, within Rru_A1593 at 1877.170 kb on - strand, within Rru_A1593 at 1877.171 kb on + strand, within Rru_A1593 at 1877.172 kb on - strand, within Rru_A1593 at 1877.172 kb on - strand, within Rru_A1593 at 1877.172 kb on - strand, within Rru_A1593 at 1877.172 kb on - strand, within Rru_A1593 at 1877.291 kb on - strand, within Rru_A1593 at 1877.291 kb on - strand, within Rru_A1593 at 1877.291 kb on - strand, within Rru_A1593 at 1877.349 kb on - strand, within Rru_A1593 at 1877.349 kb on - strand, within Rru_A1593 at 1877.355 kb on - strand, within Rru_A1593 at 1877.429 kb on + strand, within Rru_A1593 at 1877.429 kb on + strand, within Rru_A1593 at 1877.430 kb on - strand, within Rru_A1593 at 1877.430 kb on - strand, within Rru_A1593 at 1877.559 kb on + strand, within Rru_A1593 at 1877.559 kb on + strand, within Rru_A1593 at 1877.560 kb on - strand, within Rru_A1593
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 9 uM remove 1,874,543 + -0.1 1,874,543 + -0.1 1,874,545 + +1.0 1,874,545 + +1.0 1,874,545 + -0.1 1,874,546 - -0.1 1,874,546 - +1.7 1,876,627 + +2.3 1,876,628 - +1.3 1,876,802 + -1.6 1,876,990 - Rru_A1593 0.19 -1.1 1,877,040 - Rru_A1593 0.23 -2.7 1,877,169 + Rru_A1593 0.35 -1.1 1,877,169 + Rru_A1593 0.35 -1.1 1,877,169 + Rru_A1593 0.35 -0.1 1,877,169 + Rru_A1593 0.35 -0.1 1,877,169 + Rru_A1593 0.35 -0.1 1,877,169 + Rru_A1593 0.35 -1.7 1,877,170 - Rru_A1593 0.35 -3.3 1,877,170 - Rru_A1593 0.35 -0.1 1,877,171 + Rru_A1593 0.35 -2.1 1,877,172 - Rru_A1593 0.35 -0.1 1,877,172 - Rru_A1593 0.35 -2.5 1,877,172 - Rru_A1593 0.35 -0.7 1,877,172 - Rru_A1593 0.35 -2.1 1,877,291 - Rru_A1593 0.46 -2.5 1,877,291 - Rru_A1593 0.46 -0.1 1,877,291 - Rru_A1593 0.46 -0.1 1,877,349 - Rru_A1593 0.51 -1.1 1,877,349 - Rru_A1593 0.51 -1.7 1,877,355 - Rru_A1593 0.52 -0.1 1,877,429 + Rru_A1593 0.58 -0.1 1,877,429 + Rru_A1593 0.58 -2.1 1,877,430 - Rru_A1593 0.58 -1.1 1,877,430 - Rru_A1593 0.58 -1.0 1,877,559 + Rru_A1593 0.70 -0.1 1,877,559 + Rru_A1593 0.70 -0.9 1,877,560 - Rru_A1593 0.70 -0.9
Or see this region's nucleotide sequence