Experiment: Succinic Acid; Light intensity 9 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1320 and Rru_A1321 are separated by 101 nucleotides Rru_A1321 and Rru_A1322 are separated by 32 nucleotides Rru_A1322 and Rru_A1323 are separated by 17 nucleotides Rru_A1323 and Rru_A1324 are separated by 13 nucleotides
Rru_A1320: Rru_A1320 - Na+-transporting methylmalonyl-CoA/oxaloacetate decarboxylase, beta subunit (NCBI), at 1,558,503 to 1,559,714
_A1320
Rru_A1321: Rru_A1321 - Biotin--acetyl-CoA-carboxylase ligase (NCBI), at 1,559,816 to 1,560,649
_A1321
Rru_A1322: Rru_A1322 - alpha subunit of malonate decarboxylase (NCBI), at 1,560,682 to 1,562,355
_A1322
Rru_A1323: Rru_A1323 - Malonate decarboxylase delta subunit (NCBI), at 1,562,373 to 1,562,690
_A1323
Rru_A1324: Rru_A1324 - beta subunit of malonate decarboxylase (NCBI), at 1,562,704 to 1,563,660
_A1324
Position (kb)
1560
1561
1562
1563 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1559.720 kb on - strand at 1559.720 kb on - strand at 1559.804 kb on - strand at 1560.195 kb on + strand, within Rru_A1321 at 1560.296 kb on - strand, within Rru_A1321 at 1560.696 kb on + strand at 1560.730 kb on - strand at 1560.894 kb on + strand, within Rru_A1322 at 1560.895 kb on - strand, within Rru_A1322 at 1561.081 kb on + strand, within Rru_A1322 at 1561.082 kb on - strand, within Rru_A1322 at 1561.082 kb on - strand, within Rru_A1322 at 1561.103 kb on - strand, within Rru_A1322 at 1561.144 kb on + strand, within Rru_A1322 at 1561.145 kb on - strand, within Rru_A1322 at 1561.146 kb on + strand, within Rru_A1322 at 1561.147 kb on - strand, within Rru_A1322 at 1561.147 kb on - strand, within Rru_A1322 at 1561.147 kb on - strand, within Rru_A1322 at 1561.147 kb on - strand, within Rru_A1322 at 1561.171 kb on + strand, within Rru_A1322 at 1561.171 kb on + strand, within Rru_A1322 at 1561.171 kb on + strand, within Rru_A1322 at 1561.171 kb on + strand, within Rru_A1322 at 1561.171 kb on + strand, within Rru_A1322 at 1561.172 kb on - strand, within Rru_A1322 at 1561.172 kb on - strand, within Rru_A1322 at 1561.417 kb on + strand, within Rru_A1322 at 1561.418 kb on - strand, within Rru_A1322 at 1561.426 kb on + strand, within Rru_A1322 at 1561.495 kb on + strand, within Rru_A1322 at 1561.496 kb on - strand, within Rru_A1322 at 1561.496 kb on - strand, within Rru_A1322 at 1561.536 kb on + strand, within Rru_A1322 at 1561.536 kb on + strand, within Rru_A1322 at 1561.536 kb on + strand, within Rru_A1322 at 1561.536 kb on + strand, within Rru_A1322 at 1561.537 kb on - strand, within Rru_A1322 at 1561.537 kb on - strand, within Rru_A1322 at 1561.633 kb on + strand, within Rru_A1322 at 1561.633 kb on + strand, within Rru_A1322 at 1561.634 kb on - strand, within Rru_A1322 at 1561.634 kb on - strand, within Rru_A1322 at 1561.720 kb on + strand, within Rru_A1322 at 1561.720 kb on + strand, within Rru_A1322 at 1561.720 kb on + strand, within Rru_A1322 at 1561.721 kb on - strand, within Rru_A1322 at 1562.054 kb on - strand, within Rru_A1322 at 1562.054 kb on - strand, within Rru_A1322 at 1562.098 kb on + strand, within Rru_A1322 at 1562.098 kb on + strand, within Rru_A1322 at 1562.098 kb on + strand, within Rru_A1322 at 1562.098 kb on + strand, within Rru_A1322 at 1562.099 kb on - strand, within Rru_A1322 at 1562.323 kb on + strand at 1562.323 kb on + strand at 1562.324 kb on - strand at 1562.324 kb on - strand at 1562.356 kb on + strand at 1562.356 kb on + strand at 1562.688 kb on + strand at 1562.688 kb on + strand at 1562.688 kb on + strand at 1562.704 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 9 uM remove 1,559,720 - -1.4 1,559,720 - +0.5 1,559,804 - -0.1 1,560,195 + Rru_A1321 0.45 -0.3 1,560,296 - Rru_A1321 0.58 +1.2 1,560,696 + +1.0 1,560,730 - +1.3 1,560,894 + Rru_A1322 0.13 +1.0 1,560,895 - Rru_A1322 0.13 +0.7 1,561,081 + Rru_A1322 0.24 +1.6 1,561,082 - Rru_A1322 0.24 +0.5 1,561,082 - Rru_A1322 0.24 -0.1 1,561,103 - Rru_A1322 0.25 -0.1 1,561,144 + Rru_A1322 0.28 -0.1 1,561,145 - Rru_A1322 0.28 -0.1 1,561,146 + Rru_A1322 0.28 -0.1 1,561,147 - Rru_A1322 0.28 -0.1 1,561,147 - Rru_A1322 0.28 -0.1 1,561,147 - Rru_A1322 0.28 +1.5 1,561,147 - Rru_A1322 0.28 +1.9 1,561,171 + Rru_A1322 0.29 +0.7 1,561,171 + Rru_A1322 0.29 +0.2 1,561,171 + Rru_A1322 0.29 -0.9 1,561,171 + Rru_A1322 0.29 +1.1 1,561,171 + Rru_A1322 0.29 +0.9 1,561,172 - Rru_A1322 0.29 +0.7 1,561,172 - Rru_A1322 0.29 -0.4 1,561,417 + Rru_A1322 0.44 +0.6 1,561,418 - Rru_A1322 0.44 -0.1 1,561,426 + Rru_A1322 0.44 -1.1 1,561,495 + Rru_A1322 0.49 +1.5 1,561,496 - Rru_A1322 0.49 +1.4 1,561,496 - Rru_A1322 0.49 +1.1 1,561,536 + Rru_A1322 0.51 -1.1 1,561,536 + Rru_A1322 0.51 +0.6 1,561,536 + Rru_A1322 0.51 +0.4 1,561,536 + Rru_A1322 0.51 +0.9 1,561,537 - Rru_A1322 0.51 -0.1 1,561,537 - Rru_A1322 0.51 +0.1 1,561,633 + Rru_A1322 0.57 +0.1 1,561,633 + Rru_A1322 0.57 -0.1 1,561,634 - Rru_A1322 0.57 -0.1 1,561,634 - Rru_A1322 0.57 +0.9 1,561,720 + Rru_A1322 0.62 -0.1 1,561,720 + Rru_A1322 0.62 +0.8 1,561,720 + Rru_A1322 0.62 -1.7 1,561,721 - Rru_A1322 0.62 -0.1 1,562,054 - Rru_A1322 0.82 -0.1 1,562,054 - Rru_A1322 0.82 +1.3 1,562,098 + Rru_A1322 0.85 -2.7 1,562,098 + Rru_A1322 0.85 -0.1 1,562,098 + Rru_A1322 0.85 -0.1 1,562,098 + Rru_A1322 0.85 -0.1 1,562,099 - Rru_A1322 0.85 +1.0 1,562,323 + -0.1 1,562,323 + -1.5 1,562,324 - +0.9 1,562,324 - +1.6 1,562,356 + -0.8 1,562,356 + -2.3 1,562,688 + -1.2 1,562,688 + -2.7 1,562,688 + -0.1 1,562,704 - -1.1
Or see this region's nucleotide sequence