Experiment: Succinic Acid; Light intensity 9 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_A1162 and Rru_A1163 are separated by 32 nucleotides Rru_A1163 and Rru_A1164 overlap by 4 nucleotides Rru_A1164 and Rru_A1165 are separated by 17 nucleotides Rru_A1165 and Rru_A1166 are separated by 16 nucleotides
Rru_A1162: Rru_A1162 - Nickel-dependent hydrogenase, large subunit (NCBI), at 1,379,541 to 1,381,331
_A1162
Rru_A1163: Rru_A1163 - Nickel-dependent hydrogenase b-type cytochrome subunit (NCBI), at 1,381,364 to 1,382,083
_A1163
Rru_A1164: Rru_A1164 - Peptidase M52, hydrogenase expression/formation protein (NCBI), at 1,382,080 to 1,382,676
_A1164
Rru_A1165: Rru_A1165 - Hydrogenase expression/formation protein (HUPF/HYPC) (NCBI), at 1,382,694 to 1,383,065
_A1165
Rru_A1166: Rru_A1166 - hydrogenase expression/formation protein hupH (NCBI), at 1,383,082 to 1,383,858
_A1166
Position (kb)
1382
1383 Strain fitness (log2 ratio)
-1
0
1
2 at 1381.113 kb on + strand, within Rru_A1162 at 1381.114 kb on - strand, within Rru_A1162 at 1381.114 kb on - strand, within Rru_A1162 at 1381.284 kb on - strand at 1381.284 kb on - strand at 1381.430 kb on + strand at 1381.430 kb on + strand at 1381.431 kb on - strand at 1381.431 kb on - strand at 1381.436 kb on + strand, within Rru_A1163 at 1381.436 kb on + strand, within Rru_A1163 at 1381.436 kb on + strand, within Rru_A1163 at 1381.436 kb on + strand, within Rru_A1163 at 1381.437 kb on - strand, within Rru_A1163 at 1381.437 kb on - strand, within Rru_A1163 at 1381.437 kb on - strand, within Rru_A1163 at 1381.437 kb on - strand, within Rru_A1163 at 1381.437 kb on - strand, within Rru_A1163 at 1381.505 kb on + strand, within Rru_A1163 at 1381.506 kb on - strand, within Rru_A1163 at 1381.655 kb on + strand, within Rru_A1163 at 1381.656 kb on - strand, within Rru_A1163 at 1381.731 kb on - strand, within Rru_A1163 at 1381.760 kb on + strand, within Rru_A1163 at 1381.761 kb on - strand, within Rru_A1163 at 1381.772 kb on - strand, within Rru_A1163 at 1381.851 kb on - strand, within Rru_A1163 at 1381.851 kb on - strand, within Rru_A1163 at 1381.991 kb on + strand, within Rru_A1163 at 1381.992 kb on - strand, within Rru_A1163 at 1381.992 kb on - strand, within Rru_A1163 at 1382.225 kb on + strand, within Rru_A1164 at 1382.226 kb on - strand, within Rru_A1164 at 1382.270 kb on - strand, within Rru_A1164 at 1382.662 kb on + strand at 1382.693 kb on + strand at 1382.693 kb on + strand at 1382.693 kb on + strand at 1382.694 kb on - strand at 1382.694 kb on - strand at 1382.730 kb on - strand at 1383.165 kb on + strand, within Rru_A1166 at 1383.165 kb on + strand, within Rru_A1166 at 1383.166 kb on - strand, within Rru_A1166 at 1383.166 kb on - strand, within Rru_A1166 at 1383.166 kb on - strand, within Rru_A1166 at 1383.166 kb on - strand, within Rru_A1166 at 1383.166 kb on - strand, within Rru_A1166 at 1383.359 kb on + strand, within Rru_A1166 at 1383.360 kb on - strand, within Rru_A1166
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 9 uM remove 1,381,113 + Rru_A1162 0.88 +1.5 1,381,114 - Rru_A1162 0.88 +0.9 1,381,114 - Rru_A1162 0.88 -0.1 1,381,284 - +1.2 1,381,284 - +1.2 1,381,430 + +1.3 1,381,430 + -0.1 1,381,431 - -0.1 1,381,431 - +1.4 1,381,436 + Rru_A1163 0.10 -0.1 1,381,436 + Rru_A1163 0.10 -0.1 1,381,436 + Rru_A1163 0.10 +1.1 1,381,436 + Rru_A1163 0.10 +1.2 1,381,437 - Rru_A1163 0.10 +1.8 1,381,437 - Rru_A1163 0.10 +2.1 1,381,437 - Rru_A1163 0.10 +0.8 1,381,437 - Rru_A1163 0.10 +1.0 1,381,437 - Rru_A1163 0.10 -0.1 1,381,505 + Rru_A1163 0.20 +1.1 1,381,506 - Rru_A1163 0.20 +1.6 1,381,655 + Rru_A1163 0.40 -1.1 1,381,656 - Rru_A1163 0.41 +0.8 1,381,731 - Rru_A1163 0.51 +1.0 1,381,760 + Rru_A1163 0.55 +1.1 1,381,761 - Rru_A1163 0.55 -0.1 1,381,772 - Rru_A1163 0.57 +0.9 1,381,851 - Rru_A1163 0.68 +0.7 1,381,851 - Rru_A1163 0.68 +1.1 1,381,991 + Rru_A1163 0.87 +1.8 1,381,992 - Rru_A1163 0.87 +0.9 1,381,992 - Rru_A1163 0.87 +1.0 1,382,225 + Rru_A1164 0.24 -0.1 1,382,226 - Rru_A1164 0.24 -1.1 1,382,270 - Rru_A1164 0.32 -0.1 1,382,662 + -0.1 1,382,693 + -0.1 1,382,693 + -0.1 1,382,693 + -0.1 1,382,694 - +0.6 1,382,694 - +1.0 1,382,730 - +1.3 1,383,165 + Rru_A1166 0.11 +1.3 1,383,165 + Rru_A1166 0.11 -0.1 1,383,166 - Rru_A1166 0.11 +0.9 1,383,166 - Rru_A1166 0.11 +1.4 1,383,166 - Rru_A1166 0.11 +0.8 1,383,166 - Rru_A1166 0.11 -0.1 1,383,166 - Rru_A1166 0.11 +1.2 1,383,359 + Rru_A1166 0.36 +1.6 1,383,360 - Rru_A1166 0.36 +0.8
Or see this region's nucleotide sequence