Experiment: Succinic Acid; Light intensity 9 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Rru_B0047 and Rru_B0048 overlap by 4 nucleotides Rru_B0048 and Rru_B0049 overlap by 4 nucleotides Rru_B0049 and Rru_B0050 are separated by 2 nucleotides
Rru_B0047: Rru_B0047 - NAD-dependent epimerase/dehydratase (NCBI), at 49,682 to 50,716
_B0047
Rru_B0048: Rru_B0048 - Glucose-1-phosphate thymidylyltransferase, long form (NCBI), at 50,713 to 51,597
_B0048
Rru_B0049: Rru_B0049 - dTDP-4-dehydrorhamnose reductase (NCBI), at 51,594 to 52,526
_B0049
Rru_B0050: Rru_B0050 - dTDP-glucose 4,6-dehydratase (NCBI), at 52,529 to 53,584
_B0050
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-1
0
1
2
3 at 49.730 kb on + strand at 49.801 kb on + strand, within Rru_B0047 at 49.803 kb on + strand, within Rru_B0047 at 49.880 kb on + strand, within Rru_B0047 at 50.045 kb on + strand, within Rru_B0047 at 50.045 kb on + strand, within Rru_B0047 at 50.046 kb on - strand, within Rru_B0047 at 50.114 kb on + strand, within Rru_B0047 at 50.145 kb on + strand, within Rru_B0047 at 50.145 kb on + strand, within Rru_B0047 at 50.145 kb on + strand, within Rru_B0047 at 50.146 kb on - strand, within Rru_B0047 at 50.146 kb on - strand, within Rru_B0047 at 50.146 kb on - strand, within Rru_B0047 at 50.147 kb on + strand, within Rru_B0047 at 50.147 kb on + strand, within Rru_B0047 at 50.147 kb on + strand, within Rru_B0047 at 50.147 kb on + strand, within Rru_B0047 at 50.147 kb on + strand, within Rru_B0047 at 50.148 kb on - strand, within Rru_B0047 at 50.171 kb on + strand, within Rru_B0047 at 50.172 kb on - strand, within Rru_B0047 at 50.357 kb on + strand, within Rru_B0047 at 50.357 kb on + strand, within Rru_B0047 at 50.358 kb on - strand, within Rru_B0047 at 50.358 kb on - strand, within Rru_B0047 at 50.406 kb on - strand, within Rru_B0047 at 50.406 kb on - strand, within Rru_B0047 at 50.407 kb on + strand, within Rru_B0047 at 50.407 kb on + strand, within Rru_B0047 at 51.859 kb on + strand, within Rru_B0049 at 51.860 kb on - strand, within Rru_B0049 at 52.567 kb on - strand at 52.567 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Succinic Acid; Light intensity 9 uM remove 49,730 + +0.6 49,801 + Rru_B0047 0.11 +1.1 49,803 + Rru_B0047 0.12 -0.4 49,880 + Rru_B0047 0.19 +0.4 50,045 + Rru_B0047 0.35 +0.6 50,045 + Rru_B0047 0.35 +0.7 50,046 - Rru_B0047 0.35 +2.8 50,114 + Rru_B0047 0.42 +0.6 50,145 + Rru_B0047 0.45 +0.5 50,145 + Rru_B0047 0.45 +2.2 50,145 + Rru_B0047 0.45 +1.2 50,146 - Rru_B0047 0.45 +1.2 50,146 - Rru_B0047 0.45 +1.4 50,146 - Rru_B0047 0.45 +0.9 50,147 + Rru_B0047 0.45 +0.6 50,147 + Rru_B0047 0.45 +0.6 50,147 + Rru_B0047 0.45 +0.6 50,147 + Rru_B0047 0.45 +1.1 50,147 + Rru_B0047 0.45 +1.8 50,148 - Rru_B0047 0.45 +1.3 50,171 + Rru_B0047 0.47 +0.6 50,172 - Rru_B0047 0.47 +1.0 50,357 + Rru_B0047 0.65 +0.6 50,357 + Rru_B0047 0.65 +1.1 50,358 - Rru_B0047 0.65 +0.6 50,358 - Rru_B0047 0.65 +0.6 50,406 - Rru_B0047 0.70 -0.9 50,406 - Rru_B0047 0.70 +1.1 50,407 + Rru_B0047 0.70 +0.6 50,407 + Rru_B0047 0.70 +0.6 51,859 + Rru_B0049 0.28 +0.6 51,860 - Rru_B0049 0.29 +0.6 52,567 - +0.6 52,567 - +0.6
Or see this region's nucleotide sequence