Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS26725

Experiment: L-Leucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS26720 and BWI76_RS26725 are separated by 119 nucleotidesBWI76_RS26725 and BWI76_RS26730 are separated by 250 nucleotides BWI76_RS26720: BWI76_RS26720 - short-chain dehydrogenase/reductase SDR, at 5,532,695 to 5,533,453 _RS26720 BWI76_RS26725: BWI76_RS26725 - LysR family transcriptional regulator, at 5,533,573 to 5,534,472 _RS26725 BWI76_RS26730: BWI76_RS26730 - inner membrane protein YhjD, at 5,534,723 to 5,535,754 _RS26730 Position (kb) 5533 5534 5535Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 5532.593 kb on + strandat 5532.613 kb on - strandat 5532.628 kb on + strandat 5532.628 kb on + strandat 5532.628 kb on + strandat 5532.630 kb on + strandat 5532.655 kb on - strandat 5532.659 kb on + strandat 5532.664 kb on + strandat 5532.672 kb on - strandat 5532.672 kb on - strandat 5532.672 kb on - strandat 5532.672 kb on - strandat 5532.674 kb on + strandat 5532.683 kb on - strandat 5532.691 kb on - strandat 5532.710 kb on + strandat 5532.710 kb on + strandat 5532.718 kb on + strandat 5532.726 kb on - strandat 5532.726 kb on - strandat 5532.726 kb on - strandat 5532.726 kb on - strandat 5532.748 kb on + strandat 5532.748 kb on + strandat 5532.751 kb on - strandat 5532.751 kb on - strandat 5532.751 kb on - strandat 5532.756 kb on - strandat 5532.796 kb on + strand, within BWI76_RS26720at 5532.801 kb on + strand, within BWI76_RS26720at 5532.804 kb on - strand, within BWI76_RS26720at 5532.809 kb on - strand, within BWI76_RS26720at 5532.809 kb on - strand, within BWI76_RS26720at 5532.846 kb on + strand, within BWI76_RS26720at 5532.854 kb on - strand, within BWI76_RS26720at 5532.854 kb on - strand, within BWI76_RS26720at 5532.980 kb on + strand, within BWI76_RS26720at 5532.988 kb on - strand, within BWI76_RS26720at 5533.070 kb on + strand, within BWI76_RS26720at 5533.092 kb on + strand, within BWI76_RS26720at 5533.162 kb on - strand, within BWI76_RS26720at 5533.232 kb on + strandat 5533.599 kb on + strandat 5533.607 kb on - strandat 5533.610 kb on - strandat 5533.610 kb on - strandat 5533.646 kb on + strandat 5533.654 kb on - strandat 5533.654 kb on - strandat 5533.700 kb on + strand, within BWI76_RS26725at 5533.755 kb on - strand, within BWI76_RS26725at 5533.778 kb on + strand, within BWI76_RS26725at 5533.804 kb on - strand, within BWI76_RS26725at 5533.818 kb on + strand, within BWI76_RS26725at 5533.826 kb on - strand, within BWI76_RS26725at 5533.840 kb on - strand, within BWI76_RS26725at 5533.937 kb on + strand, within BWI76_RS26725at 5533.984 kb on + strand, within BWI76_RS26725at 5533.984 kb on + strand, within BWI76_RS26725at 5533.992 kb on - strand, within BWI76_RS26725at 5533.992 kb on - strand, within BWI76_RS26725at 5534.021 kb on + strand, within BWI76_RS26725at 5534.067 kb on - strand, within BWI76_RS26725at 5534.177 kb on + strand, within BWI76_RS26725at 5534.177 kb on + strand, within BWI76_RS26725at 5534.393 kb on - strandat 5534.430 kb on - strandat 5534.430 kb on - strandat 5534.531 kb on - strandat 5534.589 kb on + strandat 5534.597 kb on - strandat 5534.597 kb on - strandat 5534.753 kb on + strandat 5534.811 kb on + strandat 5534.883 kb on + strand, within BWI76_RS26730at 5534.884 kb on + strandat 5535.139 kb on + strand, within BWI76_RS26730at 5535.139 kb on + strand, within BWI76_RS26730at 5535.247 kb on - strand, within BWI76_RS26730at 5535.328 kb on + strand, within BWI76_RS26730at 5535.328 kb on + strand, within BWI76_RS26730at 5535.336 kb on - strand, within BWI76_RS26730at 5535.388 kb on + strand, within BWI76_RS26730at 5535.412 kb on + strand, within BWI76_RS26730

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Leucine (N)
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5,532,593 + -0.7
5,532,613 - -0.2
5,532,628 + +2.0
5,532,628 + -0.0
5,532,628 + +0.2
5,532,630 + -0.1
5,532,655 - +0.4
5,532,659 + +0.1
5,532,664 + +0.7
5,532,672 - +0.1
5,532,672 - -1.0
5,532,672 - +0.6
5,532,672 - +1.4
5,532,674 + -0.0
5,532,683 - -0.0
5,532,691 - +0.5
5,532,710 + -1.3
5,532,710 + +0.4
5,532,718 + -0.7
5,532,726 - -0.3
5,532,726 - -0.8
5,532,726 - -1.3
5,532,726 - -1.0
5,532,748 + -1.6
5,532,748 + -0.4
5,532,751 - +0.5
5,532,751 - -0.1
5,532,751 - -0.1
5,532,756 - -0.1
5,532,796 + BWI76_RS26720 0.13 -0.3
5,532,801 + BWI76_RS26720 0.14 -0.4
5,532,804 - BWI76_RS26720 0.14 +0.1
5,532,809 - BWI76_RS26720 0.15 +1.3
5,532,809 - BWI76_RS26720 0.15 +0.4
5,532,846 + BWI76_RS26720 0.20 -0.6
5,532,854 - BWI76_RS26720 0.21 -0.1
5,532,854 - BWI76_RS26720 0.21 -0.1
5,532,980 + BWI76_RS26720 0.38 -1.0
5,532,988 - BWI76_RS26720 0.39 -0.1
5,533,070 + BWI76_RS26720 0.49 -0.2
5,533,092 + BWI76_RS26720 0.52 -0.3
5,533,162 - BWI76_RS26720 0.62 +0.4
5,533,232 + +4.0
5,533,599 + -0.2
5,533,607 - +0.9
5,533,610 - -0.3
5,533,610 - +0.4
5,533,646 + +0.2
5,533,654 - -0.3
5,533,654 - -0.3
5,533,700 + BWI76_RS26725 0.14 +0.1
5,533,755 - BWI76_RS26725 0.20 +0.1
5,533,778 + BWI76_RS26725 0.23 -0.6
5,533,804 - BWI76_RS26725 0.26 +0.6
5,533,818 + BWI76_RS26725 0.27 -0.0
5,533,826 - BWI76_RS26725 0.28 -0.3
5,533,840 - BWI76_RS26725 0.30 -0.3
5,533,937 + BWI76_RS26725 0.40 -0.1
5,533,984 + BWI76_RS26725 0.46 +1.4
5,533,984 + BWI76_RS26725 0.46 +0.3
5,533,992 - BWI76_RS26725 0.47 -0.2
5,533,992 - BWI76_RS26725 0.47 -0.5
5,534,021 + BWI76_RS26725 0.50 -0.0
5,534,067 - BWI76_RS26725 0.55 -0.3
5,534,177 + BWI76_RS26725 0.67 -0.2
5,534,177 + BWI76_RS26725 0.67 +0.1
5,534,393 - -0.1
5,534,430 - +0.1
5,534,430 - +0.4
5,534,531 - -0.0
5,534,589 + -2.0
5,534,597 - +0.4
5,534,597 - -0.3
5,534,753 + +1.1
5,534,811 + -0.3
5,534,883 + BWI76_RS26730 0.16 -1.0
5,534,884 + +1.4
5,535,139 + BWI76_RS26730 0.40 +0.6
5,535,139 + BWI76_RS26730 0.40 -0.9
5,535,247 - BWI76_RS26730 0.51 +0.3
5,535,328 + BWI76_RS26730 0.59 -0.6
5,535,328 + BWI76_RS26730 0.59 +2.8
5,535,336 - BWI76_RS26730 0.59 -0.4
5,535,388 + BWI76_RS26730 0.64 +0.3
5,535,412 + BWI76_RS26730 0.67 +0.6

Or see this region's nucleotide sequence