Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22020 and BWI76_RS22025 are separated by 36 nucleotides BWI76_RS22025 and BWI76_RS22030 are separated by 149 nucleotides BWI76_RS22030 and BWI76_RS22035 are separated by 63 nucleotides BWI76_RS22035 and BWI76_RS22040 are separated by 87 nucleotides BWI76_RS22040 and BWI76_RS22045 are separated by 183 nucleotides BWI76_RS22045 and BWI76_RS22050 overlap by 4 nucleotides
BWI76_RS22020: BWI76_RS22020 - L-alanine exporter AlaE, at 4,598,899 to 4,599,354
_RS22020
BWI76_RS22025: BWI76_RS22025 - hypothetical protein, at 4,599,391 to 4,599,741
_RS22025
BWI76_RS22030: BWI76_RS22030 - membrane protein, at 4,599,891 to 4,600,217
_RS22030
BWI76_RS22035: BWI76_RS22035 - DeoR family transcriptional regulator, at 4,600,281 to 4,601,639
_RS22035
BWI76_RS22040: BWI76_RS22040 - carboxymuconolactone decarboxylase family protein, at 4,601,727 to 4,602,158
_RS22040
BWI76_RS22045: BWI76_RS22045 - NrdH-redoxin, at 4,602,342 to 4,602,587
_RS22045
BWI76_RS22050: BWI76_RS22050 - class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI, at 4,602,584 to 4,602,994
_RS22050
Position (kb) 4600
4601
4602 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4599.302 kb on + strand at 4599.337 kb on + strand at 4599.346 kb on - strand at 4599.351 kb on - strand at 4599.383 kb on - strand at 4599.402 kb on - strand at 4599.458 kb on - strand, within BWI76_RS22025 at 4599.470 kb on + strand, within BWI76_RS22025 at 4599.470 kb on + strand, within BWI76_RS22025 at 4599.470 kb on + strand, within BWI76_RS22025 at 4599.470 kb on + strand, within BWI76_RS22025 at 4599.478 kb on - strand, within BWI76_RS22025 at 4599.478 kb on - strand, within BWI76_RS22025 at 4599.552 kb on + strand, within BWI76_RS22025 at 4599.558 kb on + strand, within BWI76_RS22025 at 4599.558 kb on + strand, within BWI76_RS22025 at 4599.560 kb on - strand, within BWI76_RS22025 at 4599.563 kb on + strand, within BWI76_RS22025 at 4599.563 kb on + strand, within BWI76_RS22025 at 4599.566 kb on - strand, within BWI76_RS22025 at 4599.571 kb on - strand, within BWI76_RS22025 at 4599.571 kb on - strand, within BWI76_RS22025 at 4599.581 kb on - strand, within BWI76_RS22025 at 4599.607 kb on + strand, within BWI76_RS22025 at 4599.607 kb on + strand, within BWI76_RS22025 at 4599.607 kb on + strand, within BWI76_RS22025 at 4599.607 kb on + strand, within BWI76_RS22025 at 4599.614 kb on - strand, within BWI76_RS22025 at 4599.615 kb on - strand, within BWI76_RS22025 at 4599.615 kb on - strand, within BWI76_RS22025 at 4599.615 kb on - strand, within BWI76_RS22025 at 4599.618 kb on - strand, within BWI76_RS22025 at 4599.651 kb on + strand, within BWI76_RS22025 at 4599.652 kb on + strand, within BWI76_RS22025 at 4599.658 kb on + strand, within BWI76_RS22025 at 4599.659 kb on - strand, within BWI76_RS22025 at 4599.659 kb on - strand, within BWI76_RS22025 at 4599.660 kb on - strand, within BWI76_RS22025 at 4599.660 kb on - strand, within BWI76_RS22025 at 4599.661 kb on - strand, within BWI76_RS22025 at 4599.666 kb on - strand, within BWI76_RS22025 at 4599.681 kb on + strand, within BWI76_RS22025 at 4599.767 kb on + strand at 4599.768 kb on + strand at 4599.768 kb on + strand at 4599.769 kb on + strand at 4599.775 kb on - strand at 4599.776 kb on - strand at 4599.776 kb on - strand at 4599.776 kb on - strand at 4599.776 kb on - strand at 4599.779 kb on + strand at 4599.781 kb on - strand at 4599.781 kb on - strand at 4599.782 kb on - strand at 4599.782 kb on - strand at 4599.823 kb on + strand at 4599.831 kb on - strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on + strand at 4599.858 kb on - strand at 4599.858 kb on - strand at 4599.866 kb on - strand at 4599.866 kb on - strand at 4599.866 kb on - strand at 4599.866 kb on - strand at 4599.866 kb on - strand at 4599.866 kb on - strand at 4599.866 kb on - strand at 4599.874 kb on + strand at 4599.906 kb on + strand at 4599.906 kb on + strand at 4599.914 kb on - strand at 4599.914 kb on - strand at 4599.914 kb on - strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.916 kb on + strand at 4599.918 kb on + strand at 4599.918 kb on + strand at 4599.918 kb on + strand at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.924 kb on - strand, within BWI76_RS22030 at 4599.926 kb on - strand, within BWI76_RS22030 at 4599.928 kb on + strand, within BWI76_RS22030 at 4599.970 kb on + strand, within BWI76_RS22030 at 4599.978 kb on - strand, within BWI76_RS22030 at 4600.024 kb on - strand, within BWI76_RS22030 at 4600.064 kb on + strand, within BWI76_RS22030 at 4600.078 kb on - strand, within BWI76_RS22030 at 4600.110 kb on + strand, within BWI76_RS22030 at 4600.120 kb on + strand, within BWI76_RS22030 at 4600.123 kb on - strand, within BWI76_RS22030 at 4600.266 kb on + strand at 4600.418 kb on - strand, within BWI76_RS22035 at 4600.462 kb on + strand, within BWI76_RS22035 at 4600.511 kb on + strand, within BWI76_RS22035 at 4600.735 kb on + strand, within BWI76_RS22035 at 4600.873 kb on + strand, within BWI76_RS22035 at 4600.937 kb on - strand, within BWI76_RS22035 at 4600.939 kb on + strand, within BWI76_RS22035 at 4600.986 kb on + strand, within BWI76_RS22035 at 4601.128 kb on - strand, within BWI76_RS22035 at 4601.498 kb on - strand, within BWI76_RS22035 at 4601.527 kb on + strand at 4601.551 kb on - strand at 4601.671 kb on + strand at 4601.671 kb on + strand at 4601.673 kb on + strand at 4601.679 kb on - strand at 4601.783 kb on + strand, within BWI76_RS22040 at 4601.784 kb on + strand, within BWI76_RS22040 at 4601.894 kb on + strand, within BWI76_RS22040 at 4601.894 kb on - strand, within BWI76_RS22040 at 4601.896 kb on + strand, within BWI76_RS22040 at 4601.902 kb on - strand, within BWI76_RS22040 at 4601.904 kb on - strand, within BWI76_RS22040 at 4601.930 kb on - strand, within BWI76_RS22040
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 4,599,302 + +4.1 4,599,337 + +0.4 4,599,346 - +0.1 4,599,351 - +0.2 4,599,383 - -0.3 4,599,402 - +0.1 4,599,458 - BWI76_RS22025 0.19 +0.2 4,599,470 + BWI76_RS22025 0.23 -0.2 4,599,470 + BWI76_RS22025 0.23 +0.0 4,599,470 + BWI76_RS22025 0.23 +1.1 4,599,470 + BWI76_RS22025 0.23 +0.3 4,599,478 - BWI76_RS22025 0.25 +0.8 4,599,478 - BWI76_RS22025 0.25 +0.5 4,599,552 + BWI76_RS22025 0.46 +0.4 4,599,558 + BWI76_RS22025 0.48 +0.0 4,599,558 + BWI76_RS22025 0.48 -0.3 4,599,560 - BWI76_RS22025 0.48 +0.3 4,599,563 + BWI76_RS22025 0.49 -0.1 4,599,563 + BWI76_RS22025 0.49 +0.4 4,599,566 - BWI76_RS22025 0.50 +0.1 4,599,571 - BWI76_RS22025 0.51 +0.6 4,599,571 - BWI76_RS22025 0.51 +0.8 4,599,581 - BWI76_RS22025 0.54 +0.4 4,599,607 + BWI76_RS22025 0.62 -2.1 4,599,607 + BWI76_RS22025 0.62 +0.0 4,599,607 + BWI76_RS22025 0.62 -0.6 4,599,607 + BWI76_RS22025 0.62 -0.8 4,599,614 - BWI76_RS22025 0.64 -0.2 4,599,615 - BWI76_RS22025 0.64 -0.3 4,599,615 - BWI76_RS22025 0.64 +0.0 4,599,615 - BWI76_RS22025 0.64 -0.3 4,599,618 - BWI76_RS22025 0.65 +0.9 4,599,651 + BWI76_RS22025 0.74 +1.2 4,599,652 + BWI76_RS22025 0.74 -0.4 4,599,658 + BWI76_RS22025 0.76 -0.1 4,599,659 - BWI76_RS22025 0.76 +0.4 4,599,659 - BWI76_RS22025 0.76 +0.7 4,599,660 - BWI76_RS22025 0.77 -0.1 4,599,660 - BWI76_RS22025 0.77 -1.0 4,599,661 - BWI76_RS22025 0.77 +0.2 4,599,666 - BWI76_RS22025 0.78 -0.6 4,599,681 + BWI76_RS22025 0.83 +0.5 4,599,767 + +0.5 4,599,768 + -0.2 4,599,768 + +0.2 4,599,769 + +0.4 4,599,775 - +0.5 4,599,776 - -0.1 4,599,776 - -0.5 4,599,776 - -1.2 4,599,776 - +0.7 4,599,779 + -0.1 4,599,781 - -0.6 4,599,781 - +0.1 4,599,782 - -0.6 4,599,782 - -0.1 4,599,823 + -1.3 4,599,831 - +0.1 4,599,858 + -0.5 4,599,858 + -1.0 4,599,858 + +0.4 4,599,858 + +1.3 4,599,858 + -0.0 4,599,858 + -1.0 4,599,858 + +0.3 4,599,858 + +0.4 4,599,858 + +0.4 4,599,858 - +0.3 4,599,858 - -0.0 4,599,866 - +0.2 4,599,866 - -0.2 4,599,866 - -0.3 4,599,866 - +0.4 4,599,866 - -0.5 4,599,866 - +0.4 4,599,866 - -0.2 4,599,874 + -0.8 4,599,906 + +1.3 4,599,906 + +0.8 4,599,914 - -0.7 4,599,914 - +0.0 4,599,914 - -1.9 4,599,916 + -0.1 4,599,916 + +0.9 4,599,916 + +0.0 4,599,916 + +0.3 4,599,916 + -0.0 4,599,916 + +0.2 4,599,916 + -0.0 4,599,916 + +0.2 4,599,916 + -0.3 4,599,916 + -0.2 4,599,916 + -0.1 4,599,918 + -0.5 4,599,918 + -0.0 4,599,918 + -0.1 4,599,924 - BWI76_RS22030 0.10 -1.3 4,599,924 - BWI76_RS22030 0.10 -0.7 4,599,924 - BWI76_RS22030 0.10 +1.5 4,599,924 - BWI76_RS22030 0.10 +0.0 4,599,924 - BWI76_RS22030 0.10 +0.2 4,599,924 - BWI76_RS22030 0.10 +0.5 4,599,924 - BWI76_RS22030 0.10 +0.4 4,599,924 - BWI76_RS22030 0.10 -0.4 4,599,924 - BWI76_RS22030 0.10 -0.3 4,599,924 - BWI76_RS22030 0.10 -0.1 4,599,924 - BWI76_RS22030 0.10 -1.3 4,599,924 - BWI76_RS22030 0.10 -0.3 4,599,924 - BWI76_RS22030 0.10 +0.1 4,599,924 - BWI76_RS22030 0.10 +0.1 4,599,924 - BWI76_RS22030 0.10 +0.0 4,599,924 - BWI76_RS22030 0.10 -0.5 4,599,924 - BWI76_RS22030 0.10 -0.2 4,599,924 - BWI76_RS22030 0.10 +0.3 4,599,924 - BWI76_RS22030 0.10 -0.5 4,599,924 - BWI76_RS22030 0.10 -0.3 4,599,926 - BWI76_RS22030 0.11 +0.5 4,599,928 + BWI76_RS22030 0.11 -0.0 4,599,970 + BWI76_RS22030 0.24 +0.2 4,599,978 - BWI76_RS22030 0.27 -0.2 4,600,024 - BWI76_RS22030 0.41 +0.5 4,600,064 + BWI76_RS22030 0.53 -0.5 4,600,078 - BWI76_RS22030 0.57 -0.4 4,600,110 + BWI76_RS22030 0.67 -1.7 4,600,120 + BWI76_RS22030 0.70 +0.6 4,600,123 - BWI76_RS22030 0.71 -1.5 4,600,266 + -0.4 4,600,418 - BWI76_RS22035 0.10 -0.2 4,600,462 + BWI76_RS22035 0.13 +0.1 4,600,511 + BWI76_RS22035 0.17 -0.1 4,600,735 + BWI76_RS22035 0.33 +0.4 4,600,873 + BWI76_RS22035 0.44 +0.3 4,600,937 - BWI76_RS22035 0.48 -0.8 4,600,939 + BWI76_RS22035 0.48 -0.0 4,600,986 + BWI76_RS22035 0.52 -0.4 4,601,128 - BWI76_RS22035 0.62 +1.5 4,601,498 - BWI76_RS22035 0.90 +0.3 4,601,527 + +0.4 4,601,551 - -0.2 4,601,671 + +0.6 4,601,671 + +1.1 4,601,673 + -0.2 4,601,679 - -0.6 4,601,783 + BWI76_RS22040 0.13 -1.0 4,601,784 + BWI76_RS22040 0.13 +1.4 4,601,894 + BWI76_RS22040 0.39 +0.2 4,601,894 - BWI76_RS22040 0.39 +0.1 4,601,896 + BWI76_RS22040 0.39 -0.0 4,601,902 - BWI76_RS22040 0.41 -0.4 4,601,904 - BWI76_RS22040 0.41 +0.1 4,601,930 - BWI76_RS22040 0.47 -0.2
Or see this region's nucleotide sequence