Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16800 and BWI76_RS16805 are separated by 65 nucleotides BWI76_RS16805 and BWI76_RS16810 are separated by 95 nucleotides BWI76_RS16810 and BWI76_RS16815 overlap by 4 nucleotides BWI76_RS16815 and BWI76_RS16820 are separated by 16 nucleotides
BWI76_RS16800: BWI76_RS16800 - amino acid ABC transporter permease, at 3,483,355 to 3,484,119
_RS16800
BWI76_RS16805: BWI76_RS16805 - basic amino acid ABC transporter substrate-binding protein, at 3,484,185 to 3,484,952
_RS16805
BWI76_RS16810: BWI76_RS16810 - RpiR family transcriptional regulator, at 3,485,048 to 3,485,947
_RS16810
BWI76_RS16815: BWI76_RS16815 - periplasmic binding protein, at 3,485,944 to 3,486,891
_RS16815
BWI76_RS16820: BWI76_RS16820 - iron ABC transporter, at 3,486,908 to 3,487,960
_RS16820
Position (kb) 3485
3486 Strain fitness (log2 ratio) -1
0
1 at 3484.146 kb on + strand at 3484.161 kb on + strand at 3484.721 kb on - strand, within BWI76_RS16805 at 3484.881 kb on + strand at 3484.949 kb on - strand at 3485.168 kb on + strand, within BWI76_RS16810 at 3485.322 kb on - strand, within BWI76_RS16810 at 3485.639 kb on - strand, within BWI76_RS16810 at 3485.694 kb on - strand, within BWI76_RS16810 at 3486.017 kb on + strand at 3486.017 kb on + strand at 3486.032 kb on - strand at 3486.033 kb on - strand at 3486.588 kb on - strand, within BWI76_RS16815 at 3486.654 kb on + strand, within BWI76_RS16815
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 3,484,146 + +0.4 3,484,161 + +0.1 3,484,721 - BWI76_RS16805 0.70 +0.4 3,484,881 + +0.2 3,484,949 - +1.0 3,485,168 + BWI76_RS16810 0.13 +1.3 3,485,322 - BWI76_RS16810 0.30 +0.4 3,485,639 - BWI76_RS16810 0.66 +0.2 3,485,694 - BWI76_RS16810 0.72 -0.0 3,486,017 + +0.3 3,486,017 + -0.5 3,486,032 - +0.1 3,486,033 - +0.4 3,486,588 - BWI76_RS16815 0.68 +0.2 3,486,654 + BWI76_RS16815 0.75 +0.0
Or see this region's nucleotide sequence