Experiment: L-Leucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00960 and BWI76_RS00965 overlap by 4 nucleotides BWI76_RS00965 and BWI76_RS00970 are separated by 16 nucleotides BWI76_RS00970 and BWI76_RS00975 are separated by 60 nucleotides
BWI76_RS00960: BWI76_RS00960 - acetolactate synthase 2 catalytic subunit, at 195,387 to 197,033
_RS00960
BWI76_RS00965: BWI76_RS00965 - acetolactate synthase 2 small subunit, at 197,030 to 197,287
_RS00965
BWI76_RS00970: BWI76_RS00970 - branched chain amino acid aminotransferase, at 197,304 to 198,233
_RS00970
BWI76_RS00975: BWI76_RS00975 - dihydroxy-acid dehydratase, at 198,294 to 200,144
_RS00975
Position (kb) 197
198
199 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 196.541 kb on - strand, within BWI76_RS00960 at 196.597 kb on + strand at 196.605 kb on - strand, within BWI76_RS00960 at 196.754 kb on + strand, within BWI76_RS00960 at 196.869 kb on + strand at 196.933 kb on + strand at 196.941 kb on - strand at 196.973 kb on + strand at 196.981 kb on - strand at 197.236 kb on - strand, within BWI76_RS00965 at 197.330 kb on - strand at 197.383 kb on + strand at 197.399 kb on - strand, within BWI76_RS00970 at 197.484 kb on + strand, within BWI76_RS00970 at 197.484 kb on + strand, within BWI76_RS00970 at 197.540 kb on + strand, within BWI76_RS00970 at 197.540 kb on + strand, within BWI76_RS00970 at 197.686 kb on + strand, within BWI76_RS00970 at 198.044 kb on + strand, within BWI76_RS00970 at 198.112 kb on + strand, within BWI76_RS00970 at 198.168 kb on + strand at 198.297 kb on + strand at 198.442 kb on + strand at 198.442 kb on + strand at 198.543 kb on + strand, within BWI76_RS00975 at 198.551 kb on - strand, within BWI76_RS00975 at 198.608 kb on + strand, within BWI76_RS00975 at 198.636 kb on - strand, within BWI76_RS00975 at 198.648 kb on + strand, within BWI76_RS00975 at 198.849 kb on + strand, within BWI76_RS00975 at 198.850 kb on - strand, within BWI76_RS00975 at 198.850 kb on - strand, within BWI76_RS00975 at 199.017 kb on - strand, within BWI76_RS00975 at 199.040 kb on + strand, within BWI76_RS00975 at 199.043 kb on - strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975 at 199.226 kb on + strand, within BWI76_RS00975
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (N) remove 196,541 - BWI76_RS00960 0.70 -0.3 196,597 + +1.0 196,605 - BWI76_RS00960 0.74 -2.3 196,754 + BWI76_RS00960 0.83 +0.6 196,869 + +1.0 196,933 + +0.9 196,941 - +0.0 196,973 + +1.1 196,981 - -1.8 197,236 - BWI76_RS00965 0.80 -2.2 197,330 - -3.5 197,383 + +0.1 197,399 - BWI76_RS00970 0.10 -3.3 197,484 + BWI76_RS00970 0.19 -3.5 197,484 + BWI76_RS00970 0.19 -0.3 197,540 + BWI76_RS00970 0.25 +0.0 197,540 + BWI76_RS00970 0.25 -0.1 197,686 + BWI76_RS00970 0.41 -1.3 198,044 + BWI76_RS00970 0.80 -1.5 198,112 + BWI76_RS00970 0.87 -1.3 198,168 + -0.9 198,297 + -3.8 198,442 + -2.5 198,442 + -5.1 198,543 + BWI76_RS00975 0.13 -2.5 198,551 - BWI76_RS00975 0.14 -2.7 198,608 + BWI76_RS00975 0.17 -2.7 198,636 - BWI76_RS00975 0.18 -3.0 198,648 + BWI76_RS00975 0.19 -2.0 198,849 + BWI76_RS00975 0.30 -1.5 198,850 - BWI76_RS00975 0.30 -2.9 198,850 - BWI76_RS00975 0.30 -4.6 199,017 - BWI76_RS00975 0.39 -5.3 199,040 + BWI76_RS00975 0.40 +0.5 199,043 - BWI76_RS00975 0.40 -2.5 199,226 + BWI76_RS00975 0.50 -5.1 199,226 + BWI76_RS00975 0.50 -0.8 199,226 + BWI76_RS00975 0.50 -3.6 199,226 + BWI76_RS00975 0.50 -1.6
Or see this region's nucleotide sequence