Experiment: reconstituted MRS with Avantafiber 0.5 %w/v
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14065 and BBR_RS14070 are separated by 615 nucleotides BBR_RS14070 and BBR_RS14075 are separated by 387 nucleotides
BBR_RS14065: BBR_RS14065 - TnpV protein, at 930,308 to 930,681
_RS14065
BBR_RS14070: BBR_RS14070 - threonine synthase, at 931,297 to 932,787
_RS14070
BBR_RS14075: BBR_RS14075 - glutamate-5-semialdehyde dehydrogenase, at 933,175 to 934,470
_RS14075
Position (kb)
931
932
933 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 930.328 kb on + strand at 930.328 kb on + strand at 930.337 kb on + strand at 930.339 kb on + strand at 930.345 kb on - strand at 930.345 kb on - strand at 930.365 kb on + strand, within BBR_RS14065 at 930.393 kb on + strand, within BBR_RS14065 at 930.606 kb on + strand, within BBR_RS14065 at 930.606 kb on + strand, within BBR_RS14065 at 930.633 kb on + strand, within BBR_RS14065 at 930.643 kb on + strand, within BBR_RS14065 at 930.662 kb on - strand at 930.666 kb on + strand at 930.666 kb on + strand at 930.700 kb on + strand at 930.700 kb on + strand at 930.700 kb on + strand at 930.757 kb on + strand at 930.850 kb on + strand at 930.855 kb on + strand at 930.912 kb on - strand at 930.913 kb on - strand at 930.913 kb on - strand at 930.913 kb on - strand at 930.913 kb on - strand at 930.926 kb on - strand at 930.949 kb on + strand at 930.957 kb on - strand at 930.957 kb on - strand at 931.034 kb on - strand at 931.051 kb on - strand at 931.061 kb on + strand at 931.069 kb on - strand at 931.075 kb on + strand at 931.083 kb on - strand at 931.083 kb on - strand at 931.093 kb on - strand at 931.142 kb on - strand at 931.175 kb on - strand at 931.255 kb on - strand at 931.279 kb on - strand at 931.292 kb on + strand at 931.292 kb on + strand at 931.294 kb on + strand at 931.294 kb on + strand at 931.302 kb on - strand at 931.302 kb on - strand at 931.327 kb on + strand at 931.327 kb on + strand at 931.337 kb on - strand at 931.337 kb on - strand at 931.527 kb on - strand, within BBR_RS14070 at 931.565 kb on - strand, within BBR_RS14070 at 931.652 kb on - strand, within BBR_RS14070 at 931.652 kb on - strand, within BBR_RS14070 at 931.653 kb on - strand, within BBR_RS14070 at 931.698 kb on - strand, within BBR_RS14070 at 931.725 kb on + strand, within BBR_RS14070 at 931.733 kb on - strand, within BBR_RS14070 at 931.772 kb on + strand, within BBR_RS14070 at 931.785 kb on - strand, within BBR_RS14070 at 931.800 kb on - strand, within BBR_RS14070 at 931.800 kb on - strand, within BBR_RS14070 at 931.835 kb on + strand, within BBR_RS14070 at 931.858 kb on + strand, within BBR_RS14070 at 931.858 kb on + strand, within BBR_RS14070 at 932.067 kb on - strand, within BBR_RS14070 at 932.121 kb on + strand, within BBR_RS14070 at 932.155 kb on - strand, within BBR_RS14070 at 932.159 kb on - strand, within BBR_RS14070 at 932.228 kb on - strand, within BBR_RS14070 at 932.264 kb on + strand, within BBR_RS14070 at 932.270 kb on - strand, within BBR_RS14070 at 932.285 kb on + strand, within BBR_RS14070 at 932.295 kb on + strand, within BBR_RS14070 at 932.405 kb on + strand, within BBR_RS14070 at 932.448 kb on + strand, within BBR_RS14070 at 932.479 kb on - strand, within BBR_RS14070 at 932.489 kb on - strand, within BBR_RS14070 at 932.529 kb on - strand, within BBR_RS14070 at 932.539 kb on - strand, within BBR_RS14070 at 932.776 kb on - strand at 932.937 kb on + strand at 932.939 kb on + strand at 932.947 kb on - strand at 933.003 kb on + strand at 933.083 kb on - strand at 933.138 kb on - strand at 933.140 kb on + strand at 933.140 kb on + strand at 933.148 kb on - strand at 933.289 kb on + strand at 933.487 kb on - strand, within BBR_RS14075 at 933.494 kb on + strand, within BBR_RS14075 at 933.494 kb on + strand, within BBR_RS14075 at 933.494 kb on + strand, within BBR_RS14075 at 933.671 kb on + strand, within BBR_RS14075 at 933.718 kb on + strand, within BBR_RS14075 at 933.741 kb on + strand, within BBR_RS14075
Per-strain Table
Position Strand Gene LocusTag Fraction reconstituted MRS with Avantafiber 0.5 %w/v remove 930,328 + -0.4 930,328 + -0.8 930,337 + +0.2 930,339 + +0.4 930,345 - +0.2 930,345 - -0.6 930,365 + BBR_RS14065 0.15 +0.8 930,393 + BBR_RS14065 0.23 -0.3 930,606 + BBR_RS14065 0.80 -1.4 930,606 + BBR_RS14065 0.80 -0.5 930,633 + BBR_RS14065 0.87 -0.1 930,643 + BBR_RS14065 0.90 -0.3 930,662 - +1.4 930,666 + +0.1 930,666 + +0.8 930,700 + +0.3 930,700 + -0.7 930,700 + -0.6 930,757 + -0.1 930,850 + -0.0 930,855 + +0.2 930,912 - -0.0 930,913 - +0.5 930,913 - -0.5 930,913 - -0.1 930,913 - -1.2 930,926 - +0.2 930,949 + +1.5 930,957 - -0.3 930,957 - +0.8 931,034 - +0.4 931,051 - +0.3 931,061 + +0.3 931,069 - +0.1 931,075 + +0.1 931,083 - -0.3 931,083 - +0.4 931,093 - -1.0 931,142 - +0.4 931,175 - -1.1 931,255 - +0.4 931,279 - +2.4 931,292 + +0.8 931,292 + +1.0 931,294 + +1.1 931,294 + -1.2 931,302 - +0.2 931,302 - +0.8 931,327 + +0.6 931,327 + +1.2 931,337 - -2.1 931,337 - -1.3 931,527 - BBR_RS14070 0.15 +0.8 931,565 - BBR_RS14070 0.18 +0.1 931,652 - BBR_RS14070 0.24 +1.2 931,652 - BBR_RS14070 0.24 -0.4 931,653 - BBR_RS14070 0.24 -0.9 931,698 - BBR_RS14070 0.27 -3.7 931,725 + BBR_RS14070 0.29 -1.1 931,733 - BBR_RS14070 0.29 -0.5 931,772 + BBR_RS14070 0.32 -0.8 931,785 - BBR_RS14070 0.33 -0.8 931,800 - BBR_RS14070 0.34 -1.4 931,800 - BBR_RS14070 0.34 -2.3 931,835 + BBR_RS14070 0.36 +1.8 931,858 + BBR_RS14070 0.38 +1.0 931,858 + BBR_RS14070 0.38 -0.6 932,067 - BBR_RS14070 0.52 -2.0 932,121 + BBR_RS14070 0.55 +0.5 932,155 - BBR_RS14070 0.58 +0.1 932,159 - BBR_RS14070 0.58 -0.8 932,228 - BBR_RS14070 0.62 -1.1 932,264 + BBR_RS14070 0.65 -0.4 932,270 - BBR_RS14070 0.65 -2.0 932,285 + BBR_RS14070 0.66 +0.4 932,295 + BBR_RS14070 0.67 +1.4 932,405 + BBR_RS14070 0.74 +0.0 932,448 + BBR_RS14070 0.77 -0.4 932,479 - BBR_RS14070 0.79 +0.1 932,489 - BBR_RS14070 0.80 -0.4 932,529 - BBR_RS14070 0.83 +0.1 932,539 - BBR_RS14070 0.83 +1.2 932,776 - -0.8 932,937 + +0.6 932,939 + +1.1 932,947 - +1.2 933,003 + -0.3 933,083 - -0.5 933,138 - -1.1 933,140 + +0.6 933,140 + -1.6 933,148 - -0.8 933,289 + -1.5 933,487 - BBR_RS14075 0.24 -0.5 933,494 + BBR_RS14075 0.25 -0.4 933,494 + BBR_RS14075 0.25 -1.5 933,494 + BBR_RS14075 0.25 -0.6 933,671 + BBR_RS14075 0.38 -1.5 933,718 + BBR_RS14075 0.42 +0.1 933,741 + BBR_RS14075 0.44 -1.3
Or see this region's nucleotide sequence