Experiment: Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS02000 and AZOBR_RS02005 are separated by 466 nucleotides AZOBR_RS02005 and AZOBR_RS02010 are separated by 88 nucleotides AZOBR_RS02010 and AZOBR_RS02015 are separated by 211 nucleotides AZOBR_RS02015 and AZOBR_RS02020 overlap by 4 nucleotides
AZOBR_RS02000: AZOBR_RS02000 - 5-carboxymethyl-2-hydroxymuconate isomerase, at 410,176 to 410,859
_RS02000
AZOBR_RS02005: AZOBR_RS02005 - transcriptional regulator, at 411,326 to 411,751
_RS02005
AZOBR_RS02010: AZOBR_RS02010 - glutamine amidotransferase, at 411,840 to 412,601
_RS02010
AZOBR_RS02015: AZOBR_RS02015 - S-adenosylmethionine decarboxylase, at 412,813 to 413,322
_RS02015
AZOBR_RS02020: AZOBR_RS02020 - spermidine synthase, at 413,319 to 414,179
_RS02020
Position (kb) 411
412
413 Strain fitness (log2 ratio) -2
-1
0
1
2 at 410.852 kb on + strand at 411.100 kb on + strand at 411.100 kb on + strand at 411.101 kb on - strand at 411.216 kb on + strand at 411.216 kb on + strand at 411.227 kb on + strand at 411.228 kb on - strand at 411.228 kb on - strand at 411.228 kb on - strand at 411.232 kb on + strand at 411.232 kb on + strand at 411.232 kb on + strand at 411.233 kb on - strand at 411.233 kb on - strand at 411.359 kb on + strand at 411.599 kb on + strand, within AZOBR_RS02005 at 411.815 kb on + strand at 412.147 kb on + strand, within AZOBR_RS02010 at 412.148 kb on - strand, within AZOBR_RS02010 at 412.192 kb on + strand, within AZOBR_RS02010 at 412.519 kb on + strand, within AZOBR_RS02010 at 412.519 kb on + strand, within AZOBR_RS02010 at 412.519 kb on + strand, within AZOBR_RS02010 at 412.519 kb on + strand, within AZOBR_RS02010 at 412.519 kb on + strand, within AZOBR_RS02010 at 412.519 kb on + strand, within AZOBR_RS02010 at 412.519 kb on + strand, within AZOBR_RS02010 at 412.519 kb on + strand, within AZOBR_RS02010 at 412.519 kb on + strand, within AZOBR_RS02010 at 412.519 kb on + strand, within AZOBR_RS02010 at 412.519 kb on + strand, within AZOBR_RS02010 at 412.519 kb on + strand, within AZOBR_RS02010 at 412.520 kb on - strand, within AZOBR_RS02010 at 412.520 kb on - strand, within AZOBR_RS02010 at 412.520 kb on - strand, within AZOBR_RS02010 at 412.520 kb on - strand, within AZOBR_RS02010 at 412.520 kb on - strand, within AZOBR_RS02010 at 412.520 kb on - strand, within AZOBR_RS02010 at 412.520 kb on - strand, within AZOBR_RS02010 at 412.520 kb on - strand, within AZOBR_RS02010 at 412.758 kb on + strand at 413.361 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB remove 410,852 + +0.6 411,100 + -1.4 411,100 + -0.1 411,101 - +0.4 411,216 + +1.7 411,216 + +1.4 411,227 + -1.3 411,228 - -0.8 411,228 - +0.9 411,228 - -0.8 411,232 + -1.7 411,232 + -0.4 411,232 + +0.8 411,233 - -0.2 411,233 - +0.1 411,359 + -0.9 411,599 + AZOBR_RS02005 0.64 +2.4 411,815 + +0.5 412,147 + AZOBR_RS02010 0.40 -0.7 412,148 - AZOBR_RS02010 0.40 -0.6 412,192 + AZOBR_RS02010 0.46 -0.4 412,519 + AZOBR_RS02010 0.89 -0.1 412,519 + AZOBR_RS02010 0.89 +0.0 412,519 + AZOBR_RS02010 0.89 -1.8 412,519 + AZOBR_RS02010 0.89 -0.0 412,519 + AZOBR_RS02010 0.89 -0.2 412,519 + AZOBR_RS02010 0.89 -2.1 412,519 + AZOBR_RS02010 0.89 +0.8 412,519 + AZOBR_RS02010 0.89 +0.4 412,519 + AZOBR_RS02010 0.89 +0.2 412,519 + AZOBR_RS02010 0.89 +0.3 412,519 + AZOBR_RS02010 0.89 +1.1 412,519 + AZOBR_RS02010 0.89 +1.8 412,520 - AZOBR_RS02010 0.89 +0.8 412,520 - AZOBR_RS02010 0.89 +0.1 412,520 - AZOBR_RS02010 0.89 +0.1 412,520 - AZOBR_RS02010 0.89 +0.5 412,520 - AZOBR_RS02010 0.89 +1.0 412,520 - AZOBR_RS02010 0.89 +0.1 412,520 - AZOBR_RS02010 0.89 -0.8 412,520 - AZOBR_RS02010 0.89 -0.6 412,758 + -0.4 413,361 - -1.1
Or see this region's nucleotide sequence