Experiment: Zinc 0.8 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and Shew_2092 are separated by 4 nucleotides Shew_2092 and Shew_2093 are separated by 1 nucleotides Shew_2093 and Shew_2094 overlap by 8 nucleotides Shew_2094 and Shew_2095 overlap by 14 nucleotides
Shew_2091: thiH - thiamine biosynthesis protein ThiH (RefSeq), at 2,418,226 to 2,419,344
thiH
Shew_2092: Shew_2092 - thiazole biosynthesis family protein (RefSeq), at 2,419,349 to 2,420,122
_2092
Shew_2093: Shew_2093 - thiamine biosynthesis protein ThiS (RefSeq), at 2,420,124 to 2,420,333
_2093
Shew_2094: Shew_2094 - UBA/THIF-type NAD/FAD binding protein (RefSeq), at 2,420,326 to 2,421,174
_2094
Shew_2095: Shew_2095 - phosphomethylpyrimidine kinase (RefSeq), at 2,421,161 to 2,422,906
_2095
Position (kb)
2420
2421
2422 Strain fitness (log2 ratio)
-1
0
1 at 2419.930 kb on + strand, within Shew_2092 at 2420.359 kb on + strand at 2420.367 kb on - strand at 2420.726 kb on + strand, within Shew_2094 at 2420.810 kb on + strand, within Shew_2094 at 2420.918 kb on + strand, within Shew_2094 at 2421.046 kb on - strand, within Shew_2094 at 2421.179 kb on - strand at 2421.413 kb on - strand, within Shew_2095 at 2421.438 kb on + strand, within Shew_2095 at 2421.899 kb on + strand, within Shew_2095
Per-strain Table
Position Strand Gene LocusTag Fraction Zinc 0.8 mM remove 2,419,930 + Shew_2092 0.75 +0.1 2,420,359 + -1.0 2,420,367 - -0.1 2,420,726 + Shew_2094 0.47 -0.3 2,420,810 + Shew_2094 0.57 -0.3 2,420,918 + Shew_2094 0.70 -0.7 2,421,046 - Shew_2094 0.85 -1.3 2,421,179 - +0.1 2,421,413 - Shew_2095 0.14 +1.3 2,421,438 + Shew_2095 0.16 -0.1 2,421,899 + Shew_2095 0.42 -0.6
Or see this region's nucleotide sequence