Experiment: BHIS with Dimetridazole 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3938 and BT3939 overlap by 8 nucleotides BT3939 and BT3940 are separated by 549 nucleotides BT3940 and BT3941 are separated by 33 nucleotides
BT3938: BT3938 - ATP-dependent DNA helicase recQ (NCBI ptt file), at 5,112,537 to 5,114,441
BT3938
BT3939: BT3939 - single-stranded-DNA-specific exonuclease recJ (NCBI ptt file), at 5,114,434 to 5,116,152
BT3939
BT3940: BT3940 - hypothetical protein (NCBI ptt file), at 5,116,702 to 5,116,908
BT3940
BT3941: BT3941 - conserved hypothetical protein (NCBI ptt file), at 5,116,942 to 5,117,868
BT3941
Position (kb)
5114
5115
5116
5117 Strain fitness (log2 ratio)
-1
0
1
2
3 at 5116.361 kb on + strand at 5116.361 kb on + strand at 5116.419 kb on + strand at 5116.465 kb on - strand at 5116.465 kb on - strand at 5116.465 kb on - strand at 5116.473 kb on + strand at 5116.519 kb on + strand at 5116.519 kb on + strand at 5116.520 kb on - strand at 5116.520 kb on - strand at 5116.542 kb on - strand at 5116.564 kb on - strand at 5116.586 kb on + strand at 5116.587 kb on - strand at 5116.587 kb on - strand at 5116.654 kb on - strand at 5116.794 kb on - strand, within BT3940 at 5116.931 kb on - strand at 5116.940 kb on - strand at 5116.940 kb on - strand at 5116.956 kb on - strand at 5117.013 kb on + strand at 5117.066 kb on + strand, within BT3941 at 5117.103 kb on - strand, within BT3941 at 5117.103 kb on - strand, within BT3941 at 5117.148 kb on - strand, within BT3941
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.0125 mM remove 5,116,361 + -0.4 5,116,361 + -1.3 5,116,419 + -0.3 5,116,465 - -0.6 5,116,465 - +0.1 5,116,465 - -1.4 5,116,473 + -0.8 5,116,519 + -0.6 5,116,519 + +0.6 5,116,520 - +0.0 5,116,520 - +3.6 5,116,542 - -0.6 5,116,564 - +1.0 5,116,586 + +0.0 5,116,587 - +0.2 5,116,587 - +2.6 5,116,654 - +2.0 5,116,794 - BT3940 0.44 -0.7 5,116,931 - -0.6 5,116,940 - +0.4 5,116,940 - -0.6 5,116,956 - -1.3 5,117,013 + +0.0 5,117,066 + BT3941 0.13 -0.6 5,117,103 - BT3941 0.17 -0.7 5,117,103 - BT3941 0.17 -1.2 5,117,148 - BT3941 0.22 -0.9
Or see this region's nucleotide sequence