Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT2184

Experiment: BHIS with Dimetridazole 0.0125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT2183 and BT2184 are separated by 194 nucleotidesBT2184 and BT2185 overlap by 14 nucleotidesBT2185 and BT2186 are separated by 40 nucleotides BT2183: BT2183 - conserved hypothetical protein (NCBI ptt file), at 2,741,631 to 2,742,686 BT2183 BT2184: BT2184 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 2,742,881 to 2,743,375 BT2184 BT2185: BT2185 - hypothetical protein (NCBI ptt file), at 2,743,362 to 2,743,952 BT2185 BT2186: BT2186 - 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) (from data), at 2,743,993 to 2,745,225 BT2186 Position (kb) 2742 2743 2744Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2741.885 kb on - strand, within BT2183at 2741.901 kb on + strand, within BT2183at 2741.902 kb on - strand, within BT2183at 2741.903 kb on + strand, within BT2183at 2741.904 kb on - strand, within BT2183at 2741.945 kb on + strand, within BT2183at 2741.945 kb on + strand, within BT2183at 2741.946 kb on - strand, within BT2183at 2741.998 kb on + strand, within BT2183at 2742.160 kb on + strand, within BT2183at 2742.237 kb on - strand, within BT2183at 2742.237 kb on - strand, within BT2183at 2742.377 kb on - strand, within BT2183at 2742.470 kb on + strand, within BT2183at 2742.471 kb on - strand, within BT2183at 2742.471 kb on - strand, within BT2183at 2742.536 kb on + strand, within BT2183at 2742.537 kb on - strand, within BT2183at 2742.581 kb on - strandat 2742.679 kb on - strandat 2742.686 kb on + strandat 2742.692 kb on - strandat 2742.713 kb on + strandat 2742.743 kb on - strandat 2742.800 kb on - strandat 2742.849 kb on - strandat 2742.862 kb on - strandat 2742.903 kb on + strandat 2742.925 kb on - strandat 2742.944 kb on - strand, within BT2184at 2742.947 kb on + strand, within BT2184at 2743.014 kb on - strand, within BT2184at 2743.146 kb on + strand, within BT2184at 2743.147 kb on - strand, within BT2184at 2743.198 kb on - strand, within BT2184at 2743.198 kb on - strand, within BT2184at 2743.198 kb on - strand, within BT2184at 2743.268 kb on + strand, within BT2184at 2743.307 kb on - strand, within BT2184at 2743.400 kb on - strandat 2743.400 kb on - strandat 2743.431 kb on - strand, within BT2185at 2743.481 kb on - strand, within BT2185at 2743.481 kb on - strand, within BT2185at 2743.507 kb on + strand, within BT2185at 2743.668 kb on + strand, within BT2185at 2743.668 kb on + strand, within BT2185at 2743.738 kb on + strand, within BT2185at 2743.740 kb on + strand, within BT2185at 2743.741 kb on - strand, within BT2185at 2743.818 kb on - strand, within BT2185at 2743.818 kb on - strand, within BT2185at 2743.893 kb on + strand, within BT2185at 2743.899 kb on + strandat 2743.902 kb on + strandat 2743.983 kb on + strandat 2743.989 kb on + strandat 2743.989 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.0125 mM
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2,741,885 - BT2183 0.24 -2.7
2,741,901 + BT2183 0.26 -2.2
2,741,902 - BT2183 0.26 -3.6
2,741,903 + BT2183 0.26 -3.6
2,741,904 - BT2183 0.26 -2.5
2,741,945 + BT2183 0.30 -3.3
2,741,945 + BT2183 0.30 -2.6
2,741,946 - BT2183 0.30 -1.0
2,741,998 + BT2183 0.35 -2.9
2,742,160 + BT2183 0.50 -2.1
2,742,237 - BT2183 0.57 -0.6
2,742,237 - BT2183 0.57 -0.6
2,742,377 - BT2183 0.71 -1.8
2,742,470 + BT2183 0.79 -0.6
2,742,471 - BT2183 0.80 -1.0
2,742,471 - BT2183 0.80 -3.8
2,742,536 + BT2183 0.86 +1.0
2,742,537 - BT2183 0.86 +0.0
2,742,581 - -1.0
2,742,679 - -2.7
2,742,686 + -1.8
2,742,692 - -0.7
2,742,713 + -0.6
2,742,743 - +0.6
2,742,800 - -0.8
2,742,849 - +1.0
2,742,862 - +1.0
2,742,903 + -0.4
2,742,925 - +0.8
2,742,944 - BT2184 0.13 -1.0
2,742,947 + BT2184 0.13 -1.3
2,743,014 - BT2184 0.27 +0.7
2,743,146 + BT2184 0.54 -0.6
2,743,147 - BT2184 0.54 +0.5
2,743,198 - BT2184 0.64 +0.4
2,743,198 - BT2184 0.64 +0.4
2,743,198 - BT2184 0.64 +1.1
2,743,268 + BT2184 0.78 +1.0
2,743,307 - BT2184 0.86 -0.3
2,743,400 - -1.5
2,743,400 - +0.0
2,743,431 - BT2185 0.12 -2.0
2,743,481 - BT2185 0.20 -2.2
2,743,481 - BT2185 0.20 -0.6
2,743,507 + BT2185 0.25 +0.0
2,743,668 + BT2185 0.52 +1.0
2,743,668 + BT2185 0.52 -2.2
2,743,738 + BT2185 0.64 -2.0
2,743,740 + BT2185 0.64 -0.9
2,743,741 - BT2185 0.64 -0.9
2,743,818 - BT2185 0.77 +0.0
2,743,818 - BT2185 0.77 -0.3
2,743,893 + BT2185 0.90 +0.7
2,743,899 + -1.0
2,743,902 + +0.0
2,743,983 + -0.4
2,743,989 + -1.1
2,743,989 + +1.3

Or see this region's nucleotide sequence